UMMS Affiliation

Department of Cell Biology



Document Type


Medical Subject Headings

Carbon Isotopes; Cell Fractionation; Cytarabine; DNA; *DNA Replication; Electrophoresis, Disc; Hela Cells; Leucine; Lysine; Methods; *Mitosis; Nucleoproteins; Proteins; RNA; Thymidine; Time Factors; Tritium; Tryptophan


Cell Biology


The synthesis and accumulation of acidic proteins in the tightly bound residual nuclear fraction goes on throughout the cell cycle of continuously dividing populations of HeLa S-3 cells; however, during late G(1) there is an increased rate of synthesis and accumulation of these proteins which precedes the onset of DNA synthesis. Unlike that of the histones, whose synthesis is tightly coupled to DNA replication, the synthesis of acidic residual nuclear proteins is insensitive to inhibitors of DNA synthesis. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of acidic residual nuclear proteins shows different profiles during the G(1), S, and G(2) phases of the cell cycle. These results suggest that, in contrast to histones whose synthesis appears to be highly regulated, the acidic residual proteins may have a regulatory function in the control of cell proliferation in continuously dividing mammalian cells.

Rights and Permissions

Citation: J Cell Biol. 1972 Feb;52(2):292-307. Link to article on publisher's website

Related Resources

Link to Article in PubMed

PubMed ID


Included in

Cell Biology Commons



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