Title
Phosphorylation of nonhistone chromosomal proteins early during the prereplicative phase of the cell cycle of WI-38 human diploid fibroblasts
UMMS Affiliation
Department of Cell Biology
Publication Date
1976-04-01
Document Type
Article
Subjects
*Cell Division; Cell Line; Chromosomes; Cycloheximide; Nucleoproteins; Phosphates; RNA
Disciplines
Cell Biology
Abstract
Stimulation of confluent monolayers of nondividing WI-38 cells to proliferate is accompanied by a doubling in the rate of phosphorylation of nonhistone chromosomal proteins during the first hour. The use of cycloheximide to inhibit protein synthesis does not appear to affect phosphorylation of the nonhistones. Nondividing and serum-stimulated cells exhibit qualitative differences in the phosphorylation and dephosphorylation of individual polypeptide peaks resolved by polyacrylamide gel electrophoresis. These results are consistent with other evidence implicating nonhistone protein phosphorylation as an early event involved in the activation of transcription occurring during cell proliferation.
Source
Cell Differ. 1976 Apr;5(1):45-52.
Journal/Book/Conference Title
Cell differentiation
Related Resources
PubMed ID
1260862
Repository Citation
Pumo, D. E.; Stein, Gary S.; and Kleinsmith, Lewis J., "Phosphorylation of nonhistone chromosomal proteins early during the prereplicative phase of the cell cycle of WI-38 human diploid fibroblasts" (1976). Stein, Stein, Lian, vanWijnen Lab Publications. 201.
https://escholarship.umassmed.edu/stein/201