Synthesis of nuclear proteins during DNA repair synthesis in human diploid fibroblasts damaged with ultraviolet radiation of N-acetoxy-2-acetylaminofluroene
Department of Cell Biology
Medical Subject Headings
Acetoxyacetylaminofluorene; Cell Line; Cell Nucleus; *DNA Repair; Histones; Hydroxyurea; Nucleoproteins; Radiation Effects; Ultraviolet Rays
We have examined the accumulation of newly synthesized nuclear proteins into nuclei during DNA repair synthesis in confluent WI-38 human diploid fibroblasts damaged with ultraviolet radiation or N-acetoxy-2-acetylaminofluroene. In contrast to a marked stimulation of DNA repair synthesis, stimulation of amino acid incorporation into histone polypeptides or into the various molecular weight classes of nonhistone nuclear proteins was not observed. These results suggest that detectable stimulation of newly synthesized nuclear protein incorporation into nuclei does not accompany DNA repair synthesis induced by ultraviolet radiation or a direct acting chemical carcinogen. At least for the special case of repair, DNA synthesis may be uncoupled from histone synthesis.
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Citation: Proc Natl Acad Sci U S A. 1976 May;73(5):1466-70.
Stein, Gary S.; Park, W. D.; Stein, Janet L.; and Lieberman, Michael W., "Synthesis of nuclear proteins during DNA repair synthesis in human diploid fibroblasts damaged with ultraviolet radiation of N-acetoxy-2-acetylaminofluroene" (1976). Stein, Stein, Lian, vanWijnen Lab Publications. 200.