UMMS Affiliation

Department of Cell Biology

Publication Date


Document Type



Amanitins; Cell Cycle; Cell Nucleus; DNA-Directed RNA Polymerases; Hela Cells; Histones; Kinetics; Nucleic Acid Hybridization; RNA Polymerase II; RNA, Messenger; Transcription, Genetic


Cell Biology


Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.

DOI of Published Version



Nucleic Acids Res. 1978 May;5(5):1515-28. Link to article on publisher's website

Journal/Book/Conference Title

Nucleic acids research

Related Resources

Link to Article in PubMed

PubMed ID


Included in

Cell Biology Commons



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