Department of Cell Biology
Medical Subject Headings
Amanitins; Cell Cycle; Cell Nucleus; DNA-Directed RNA Polymerases; Hela Cells; Histones; Kinetics; Nucleic Acid Hybridization; RNA Polymerase II; RNA, Messenger; Transcription, Genetic
Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.
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Citation: Nucleic Acids Res. 1978 May;5(5):1515-28. Link to article on publisher's website
Detke, Siegfried; Stein, Janet L.; and Stein, Gary S., "Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells" (1978). Stein, Stein, Lian, vanWijnen Lab Publications. 182.