Department of Cell Biology
Amanitins; Cell Cycle; Cell Nucleus; DNA-Directed RNA Polymerases; Hela Cells; Histones; Kinetics; Nucleic Acid Hybridization; RNA Polymerase II; RNA, Messenger; Transcription, Genetic
Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.
DOI of Published Version
Nucleic Acids Res. 1978 May;5(5):1515-28. Link to article on publisher's website
Nucleic acids research
Detke S, Stein JL, Stein GS. (1978). Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3 cells. Stein, Stein, Lian, vanWijnen Lab Publications. https://doi.org/10.1093/nar/5.5.1515. Retrieved from https://escholarship.umassmed.edu/stein/182