Title
Multiple forms of H4 histone mRNA in human cells
UMMS Affiliation
Department of Cell Biology
Publication Date
1980-04-01
Document Type
Article
Subjects
Cell Cycle; Hela Cells; Histones; Humans; Molecular Weight; Nucleic Acid Conformation; Polyribosomes; RNA Caps; RNA, Messenger
Disciplines
Cell Biology
Abstract
Two species of H4 histone mRNA were isolated from the polysomes of S phase HeLa S3 cells. Electrophoresis under denaturing and nondenaturing conditions indicates that the two H4 mRNAs differ in size. Both mRNAs translate H4 histones in vitro, lack poly(A) at their 3' termini, and are capped at their 5' termini. Polyacrylamide gel electrophoresis and tryptic peptide analysis suggest that the polypeptides synthesized by the two mRNAs are indistinguishable.
Source
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1942-6.
Journal/Book/Conference Title
Proceedings of the National Academy of Sciences of the United States of America
Related Resources
PubMed ID
6929528
Repository Citation
Lichtler, A. C.; Detke, Siegfried; Phillips, Ian R.; Stein, Gary S.; and Stein, Janet L., "Multiple forms of H4 histone mRNA in human cells" (1980). Stein, Stein, Lian, vanWijnen Lab Publications. 173.
https://escholarship.umassmed.edu/stein/173