Representation of mRNA sequences in normal and SV40 transformed human diploid fibroblasts

Elizabeth A. Shephard, University of Florida
R. L. Jansing, University of Florida
Ian R. Phillips, University of Florida
Janet L. Stein, University of Massachusetts Medical School
Gary S. Stein, University of Massachusetts Medical School

Abstract

The representation of mRNA sequences in normal and SV40-transformed WI-38 human diploid fibroblasts was examined. Variations in the hybridization of polysomal RNAs to 3H-labeled DNAs complementary to poly A+ mRNA of normal and SV40-transformed cells suggest differences in the mRNA populations. a comparison of the representation of histone RNA sequences of normal and SV40-transformed human diploid cells did not reveal significant differences.