cis-Diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific
Department of Cell Biology
Medical Subject Headings
Cell Cycle; Cisplatin; Cross-Linking Reagents; DNA; Electrophoresis, Polyacrylamide Gel; Hela Cells; Humans; Immunochemistry; Nucleoproteins; Protein Binding; Thymidine
Immunochemical analysis was employed to investigate the cell cycle-dependent protein-DNA crosslinking by cis-diamminedichloroplatinum II (cis-DDP), in HeLa-S3 cells. Cells synchronized by double thymidine block or hydroxyurea were released into S phase and incubated at 2-h intervals with cis-DDP as they progressed through S1, G2, M, and then into G1 and S phases of the subsequent cycle. Immunoblots of the DNA-crosslinked antigens reacted with antisera to 0.35 M NaCl extract or residue of HeLa S-phase nuclei revealed that several antigens changed their DNA-crosslinking pattern during the progression of HeLa cells through their reproductive cycle.
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Citation: Arch Biochem Biophys. 1985 Feb 15;237(1):202-7.
Banjar, Zainy M.; Hnilica, Lobumir S.; Briggs, Robert C.; Dominguez, E.; Stein, Janet L.; and Stein, Gary S., "cis-Diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific" (1985). Stein, Stein, Lian, vanWijnen Lab Publications. 145.