cis-Diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific
Department of Cell Biology
Cell Cycle; Cisplatin; Cross-Linking Reagents; DNA; Electrophoresis, Polyacrylamide Gel; Hela Cells; Humans; Immunochemistry; Nucleoproteins; Protein Binding; Thymidine
Immunochemical analysis was employed to investigate the cell cycle-dependent protein-DNA crosslinking by cis-diamminedichloroplatinum II (cis-DDP), in HeLa-S3 cells. Cells synchronized by double thymidine block or hydroxyurea were released into S phase and incubated at 2-h intervals with cis-DDP as they progressed through S1, G2, M, and then into G1 and S phases of the subsequent cycle. Immunoblots of the DNA-crosslinked antigens reacted with antisera to 0.35 M NaCl extract or residue of HeLa S-phase nuclei revealed that several antigens changed their DNA-crosslinking pattern during the progression of HeLa cells through their reproductive cycle.
Arch Biochem Biophys. 1985 Feb 15;237(1):202-7.
Archives of biochemistry and biophysics
Banjar ZM, Hnilica LS, Briggs RC, Dominguez E, Stein JL, Stein GS. (1985). cis-Diamminedichloroplatinum-mediated crosslinking of nuclear proteins to DNA is cell cycle specific. Stein, Stein, Lian, vanWijnen Lab Publications. Retrieved from https://escholarship.umassmed.edu/stein/145