Title
Nuclear antigens in the HeLa cell cycle
UMMS Affiliation
Department of Cell Biology
Publication Date
1985-07-01
Document Type
Article
Subjects
Antigens; Antigens, Nuclear; Cell Cycle; Hela Cells; Humans; Interphase; Metaphase; Nucleoproteins
Disciplines
Cell Biology
Abstract
Antisera to 0.35 M NaCl extracts and residues of S phase HeLa nuclei were reacted with electrophoretically separated proteins from the nuclei or nuclear material of HeLa cells synchronized in G1, S, G2 or M phases of the cell cycle. Quantitative evaluation of the peroxidase-antiperoxidase stained nitrocellulose transfers (Western blots) revealed significant changes in the quantities of nuclear non-histone proteins during the cell cycle. Immunochemical staining of electrophoretically separated nuclear antigens permits their selective detection in minute quantities and in the presence of many additional proteins.
Source
Mol Cell Biochem. 1985 Jul;67(2):101-10.
Journal/Book/Conference Title
Molecular and cellular biochemistry
Related Resources
PubMed ID
4047024
Repository Citation
Banjar, Zainy M.; Briggs, Robert C.; Hnilica, Lobumir S.; Stein, Janet L.; and Stein, Gary S., "Nuclear antigens in the HeLa cell cycle" (1985). Stein, Stein, Lian, vanWijnen Lab Publications. 142.
https://escholarship.umassmed.edu/stein/142