Title

Partial liquid ventilation with perfluorocarbon in the treatment of rats with lethal pneumococcal pneumonia

UMMS Affiliation

Department of Anesthesiology; Department of Surgery; Department of Pathology

Publication Date

1998-02-03

Document Type

Article

Subjects

Animals; Fluorocarbons; Male; Penicillin G; Pneumonia, Pneumococcal; Rats; Rats, Wistar; *Respiration, Artificial

Disciplines

Anesthesiology | Life Sciences | Medicine and Health Sciences | Respiratory Tract Diseases | Surgery

Abstract

BACKGROUND: Partial liquid ventilation with perfluorocarbon is a new therapeutic strategy to treat various lung disorders. The current study was undertaken to determine the efficacy of partial liquid ventilation with a perfluorocarbon (FC-77) in the treatment of pneumococcal pneumonia in rats.

METHODS: Male Wistar rats (weight, 275-300 g; n, 75) were infected via direct intratracheal inoculation with ca 10(9) colony-forming units of viable Streptococcus pneumoniae, serotype 3, and 24 h after infection were placed into one of five groups, each containing 15 rats. The groups were (1) no treatment, (2) one intramuscular injection of penicillin G benzathine (200,000 U), (3) partial liquid ventilation with FC-77, (4) partial liquid ventilation with FC-77 and a single intramuscular dose of penicillin G benzathine (200,000 U), and (5) gas ventilation. Animals were observed every 24 h for survival.

RESULTS: All untreated or gas-ventilated animals or animals that received only partial liquid ventilation were dead by 7 days. Those receiving only partial liquid ventilation survived longer than untreated controls, but ultimately all succumbed by day 7. Survival was 40% for penicillin-treated rats compared with controls (P < 0.05) and 80% for animals treated with both partial liquid ventilation and penicillin versus antibiotic alone (P < 0.05).

CONCLUSIONS: These observations suggest that partial liquid ventilation with perfluorocarbon in combination with antibiotic administration may be an effective therapeutic modality in pneumococcal pneumonia.

Source

Anesthesiology. 1998 Jan;88(1):218-23.

Journal/Book/Conference Title

Anesthesiology

Comments

Medical student Eric Dickson participated in this study as part of the Senior Scholars research program.

Related Resources

Link to Article in PubMed

PubMed ID

9447875

Link to Full Text

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