Partial liquid ventilation with perfluorocarbon in the treatment of rats with lethal pneumococcal pneumonia
Department of Anesthesiology; Department of Surgery; Department of Pathology
Animals; Fluorocarbons; Male; Penicillin G; Pneumonia, Pneumococcal; Rats; Rats, Wistar; *Respiration, Artificial
Anesthesiology | Life Sciences | Medicine and Health Sciences | Respiratory Tract Diseases | Surgery
BACKGROUND: Partial liquid ventilation with perfluorocarbon is a new therapeutic strategy to treat various lung disorders. The current study was undertaken to determine the efficacy of partial liquid ventilation with a perfluorocarbon (FC-77) in the treatment of pneumococcal pneumonia in rats.
METHODS: Male Wistar rats (weight, 275-300 g; n, 75) were infected via direct intratracheal inoculation with ca 10(9) colony-forming units of viable Streptococcus pneumoniae, serotype 3, and 24 h after infection were placed into one of five groups, each containing 15 rats. The groups were (1) no treatment, (2) one intramuscular injection of penicillin G benzathine (200,000 U), (3) partial liquid ventilation with FC-77, (4) partial liquid ventilation with FC-77 and a single intramuscular dose of penicillin G benzathine (200,000 U), and (5) gas ventilation. Animals were observed every 24 h for survival.
RESULTS: All untreated or gas-ventilated animals or animals that received only partial liquid ventilation were dead by 7 days. Those receiving only partial liquid ventilation survived longer than untreated controls, but ultimately all succumbed by day 7. Survival was 40% for penicillin-treated rats compared with controls (P < 0.05) and 80% for animals treated with both partial liquid ventilation and penicillin versus antibiotic alone (P < 0.05).
CONCLUSIONS: These observations suggest that partial liquid ventilation with perfluorocarbon in combination with antibiotic administration may be an effective therapeutic modality in pneumococcal pneumonia.
Anesthesiology. 1998 Jan;88(1):218-23.
Dickson, Eric W.; Heard, Stephen O.; Chu, Benson; Fraire, Armando E.; Brueggemann, Angela B.; and Doern, Gary V., "Partial liquid ventilation with perfluorocarbon in the treatment of rats with lethal pneumococcal pneumonia" (1998). University of Massachusetts Medical School. Senior Scholars Program. Paper 79.