Hyperspectral Imaging for Burn Depth Assessment in an Animal Model
Authors
Chin, Michael S.Babchenko, Oksana Olegovna
Lujan-Hernandez, Jorge
Nobel, Lisa
Ignotz, Ronald A.
Lalikos, Janice F.
Faculty Advisor
Janice Lalikos, MD/Plastic SurgeryUMass Chan Affiliations
Department of Surgery, Division of Plastic SurgeryDepartment of Radiation Oncology
School of Medicine
Senior Scholars Program
Document Type
Journal ArticlePublication Date
2016-01-07
Metadata
Show full item recordAbstract
Differentiating between superficial and deep-dermal (DD) burns remains challenging. Superficial-dermal burns heal with conservative treatment; DD burns often require excision and skin grafting. Decision of surgical treatment is often delayed until burn depth is definitively identified. This study's aim is to assess the ability of hyperspectral imaging (HSI) to differentiate burn depth. METHODS: Thermal injury of graded severity was generated on the dorsum of hairless mice with a heated brass rod. Perfusion and oxygenation parameters of injured skin were measured with HSI, a noninvasive method of diffuse reflectance spectroscopy, at 2 minutes, 1, 24, 48 and 72 hours after wounding. Burn depth was measured histologically in 12 mice from each burn group (n = 72) at 72 hours. RESULTS: Three levels of burn depth were verified histologically: intermediate-dermal (ID), DD, and full-thickness. At 24 hours post injury, total hemoglobin (tHb) increased by 67% and 16% in ID and DD burns, respectively. In contrast, tHb decreased to 36% of its original levels in full-thickness burns. Differences in deoxygenated and tHb among all groups were significant (P < 0.001) at 24 hours post injury. CONCLUSIONS: HSI was able to differentiate among 3 discrete levels of burn injury. This is likely because of its correlation with skin perfusion: superficial burn injury causes an inflammatory response and increased perfusion to the burn site, whereas deeper burns destroy the dermal microvasculature and a decrease in perfusion follows. This study supports further investigation of HSI in early burn depth assessment.Source
Plast Reconstr Surg Glob Open. 2016 Jan 7;3(12):e591. doi: 10.1097/GOX.0000000000000558. eCollection 2015.. Link to article on publisher's siteDOI
10.1097/GOX.0000000000000558Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49280PubMed ID
26894016Notes
Oksana Babchenko participated in this study as a medical student as part of the Senior Scholars research program at the University of Massachusetts Medical School.
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This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.
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http://creativecommons.org/licenses/by-nc-nd/4.0/ae974a485f413a2113503eed53cd6c53
10.1097/GOX.0000000000000558
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Except where otherwise noted, this item's license is described as <p>This is an open-access article distributed under the terms of the <a href="http://creativecommons.org/licenses/by-nc-nd/4.0/">Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND)</a>, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.</p>