The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation
Authors
La Terra, SabrinaEnglish, Christopher N.
Hergert, Polla J.
McEwen, Bruce F.
Sluder, Greenfield
Khodjakov, Alexey
UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
2005-03-02
Metadata
Show full item recordAbstract
It has been reported that nontransformed mammalian cells become arrested during G1 in the absence of centrioles (Hinchcliffe, E., F. Miller, M. Cham, A. Khodjakov, and G. Sluder. 2001. Science. 291:1547-1550). Here, we show that removal of resident centrioles (by laser ablation or needle microsurgery) does not impede cell cycle progression in HeLa cells. HeLa cells born without centrosomes, later, assemble a variable number of centrioles de novo. Centriole assembly begins with the formation of small centrin aggregates that appear during the S phase. These, initially amorphous "precentrioles" become morphologically recognizable centrioles before mitosis. De novo-assembled centrioles mature (i.e., gain abilities to organize microtubules and replicate) in the next cell cycle. This maturation is not simply a time-dependent phenomenon, because de novo-formed centrioles do not mature if they are assembled in S phase-arrested cells. By selectively ablating only one centriole at a time, we find that the presence of a single centriole inhibits the assembly of additional centrioles, indicating that centrioles have an activity that suppresses the de novo pathway.Source
J Cell Biol. 2005 Feb 28;168(5):713-22. Link to article on publisher's siteDOI
10.1083/jcb.200411126Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49036PubMed ID
15738265Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1083/jcb.200411126