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UMass Chan Affiliations
Department of Cell and Developmental BiologyDocument Type
Journal ArticlePublication Date
2013-08-07Keywords
Microscopy, FluorescenceMicroscopy, Video
Optical Phenomena
Video Recording
Cell Biology
Investigative Techniques
Laboratory and Basic Science Research
Optics
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Show full item recordAbstract
This chapter provides information on how microscopes work and discusses some of the microscope issues to be considered in using a video camera on the microscope. There are two types of microscopes in use today for research in cell biology-the older finite tube-length (typically 160mm mechanical tube length) microscopes and the infinity optics microscopes that are now produced. The objective lens forms a magnified, real image of the specimen at a specific distance from the objective known as the intermediate image plane. All objectives are designed to be used with the specimen at a defined distance from the front lens element of the objective (the working distance) so that the image formed is located at a specific location in the microscope. Infinity optics microscopes differ from the finite tube-length microscopes in that the objectives are designed to project the image of the specimen to infinity and do not, on their own, form a real image of the specimen. Three types of objectives are in common use today-plan achromats, plan apochromats, and plan fluorite lenses. The concept of mounting video cameras on the microscope is also presented in the chapter.Source
Sluder G, Nordberg JJ. Microscope basics. Methods Cell Biol. 2013;114:1-10. doi: 10.1016/B978-0-12-407761-4.00001-4. Link to article on publisher's siteDOI
10.1016/B978-0-12-407761-4.00001-4Permanent Link to this Item
http://hdl.handle.net/20.500.14038/49028PubMed ID
23931500Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/B978-0-12-407761-4.00001-4