UMMS Affiliation

Department of Cell Biology

Publication Date


Document Type



Animals; Bicuculline; Brain; Cell Movement; Cell Shape; *Computer Systems; Embryo, Mammalian; GABA Antagonists; GABA-A Receptor Antagonists; Gonadotropin-Releasing Hormone; Green Fluorescent Proteins; Immunohistochemistry; Mice; Mice, Transgenic; *Microscopy, Video; Neural Pathways; Neurons; Nose; Tissue Fixation


Cell Biology


Neurons that synthesize GnRH control the reproductive axis and migrate over long distances and through different environments during development. Prior studies provided strong clues for the types of molecules encountered and movements expected along the migratory route. However, our studies provide the first real-time views of the behavior of GnRH neurons in the context of an in vitro preparation that maintains conditions comparable to those in vivo. The live views provide direct evidence of the changing behavior of GnRH neurons in their different environments, showing that GnRH neurons move with greater frequency and with more changes in direction after they enter the brain. Perturbations of guiding fibers distal to moving GnRH neurons in the nasal compartment influenced movement without detectable changes in the fibers in the immediate vicinity of moving GnRH neurons. This suggests that the use of fibers by GnRH neurons for guidance may entail selective signaling in addition to mechanical guidance. These studies establish a model to evaluate the influences of specific molecules that are important for their migration.

DOI of Published Version



Endocrinology. 2005 Jan;146(1):463-8. Epub 2004 Oct 14. Link to article on publisher's site

Journal/Book/Conference Title


Related Resources

Link to Article in PubMed

PubMed ID


Included in

Cell Biology Commons