Title

Cryo-EM Structure of CtBP2 Confirms Tetrameric Architecture

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Schiffer Lab; Graduate School of Biomedical Sciences

Publication Date

2020-11-27

Document Type

Article

Disciplines

Amino Acids, Peptides, and Proteins | Biochemistry | Cell and Developmental Biology | Medicinal Chemistry and Pharmaceutics | Medicinal-Pharmaceutical Chemistry | Molecular Biology | Structural Biology | Virology

Abstract

C-terminal binding proteins 1 and 2 (CtBP1 and CtBP2) are transcriptional regulators that activate or repress many genes involved in cellular development, apoptosis, and metastasis. NADH-dependent CtBP activation has been implicated in multiple types of cancer and poor patient prognosis. Central to understanding activation of CtBP in oncogenesis is uncovering how NADH triggers protein assembly, what level of assembly occurs, and if oncogenic activity depends upon such assembly. Here, we present the cryoelectron microscopic structures of two different constructs of CtBP2 corroborating that the native state of CtBP2 in the presence of NADH is tetrameric. The physiological relevance of the observed tetramer was demonstrated in cell culture, showing that CtBP tetramer-destabilizing mutants are defective for cell migration, transcriptional repression of E-cadherin, and activation of TIAM1. Together with our cryoelectron microscopy studies, these results highlight the tetramer as the functional oligomeric form of CtBP2.

Keywords

C-terminal binding-protein CtBP, TIAM1, cancer, metastasis, tetrameric assembly, transcription regulation

DOI of Published Version

10.1016/j.str.2020.11.008

Source

Jecrois AM, Dcona MM, Deng X, Bandyopadhyay D, Grossman SR, Schiffer CA, Royer WE Jr. Cryo-EM Structure of CtBP2 Confirms Tetrameric Architecture. Structure. 2020 Nov 27:S0969-2126(20)30420-2. doi: 10.1016/j.str.2020.11.008. Epub ahead of print. PMID: 33264605. Link to article on publisher's site

Journal/Book/Conference Title

Structure (London, England)

Comments

This article is based on a previously available preprint on bioRxiv. Version 1 of this preprint is also available in eScholarship@UMMS.

Related Resources

Link to Article in PubMed

PubMed ID

33264605

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