RNA Therapeutics Institute Publications

Title

CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute

Publication Date

2018-11-01

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Bioimaging and Biomedical Optics | Genetics and Genomics

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance brightness for live cell imaging of genomic loci. However, many of these modifications result in guide RNA instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays of aptamers conferring both enhanced guide RNA stability and brightness, and provide initial biological applications of this platform.

DOI of Published Version

10.1038/s41592-018-0174-0

Source

Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30. Link to article on publisher's site

Journal/Book/Conference Title

Nature methods

Related Resources

Link to Article in PubMed

PubMed ID

30377374

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