CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging
Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Biochemistry, Biophysics, and Structural Biology | Bioimaging and Biomedical Optics | Genetics and Genomics
Clustered regularly interspaced short palindromic repeats (CRISPR) guide RNA scaffolds have been adapted to carry multiple binding sites for fluorescent proteins to enhance brightness for live cell imaging of genomic loci. However, many of these modifications result in guide RNA instability and thus produce lower genome-labeling efficiency than anticipated. Here we introduce CRISPR-Sirius, based on octet arrays of aptamers conferring both enhanced guide RNA stability and brightness, and provide initial biological applications of this platform.
DOI of Published Version
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30. Link to article on publisher's site
Ma, Hanhui; Tu, Li-Chun; Naseri, Ardalan; Chung, Yu-Chieh; Grünwald, David; Zhang, Shaojie; and Pederson, Thoru, "CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging" (2018). RNA Therapeutics Institute Publications. 59.