RNA Therapeutics Institute Publications
Title
Impact of optical aberrations on axial position determination by photometry
UMMS Affiliation
RNA Therapeutics Institute
Publication Date
2018-12-01
Document Type
Article
Disciplines
Biochemistry, Biophysics, and Structural Biology | Bioimaging and Biomedical Optics
Abstract
Recently, Franke et al.introduced a way to estimate the axial position of single molecules (temporal radial-aperture-based intensity estimation (TRABI)). To this end, they compared the detected photon count from a TRABI estimation to the estimated count from Gaussian point-spread function (PSF) fitting to the data. Empirically they found that the photometric ratio is ~0.7–0.8 at points close to focus and decreases as the distance from the focal plane increases. Here we explain this reported but unexplained discrepancy and, furthermore, show that the photometric ratio as an indicator for axial position is susceptible even to typical optical aberrations.
DOI of Published Version
10.1038/s41592-018-0227-4
Source
Nat Methods. 2018 Dec;15(12):989-990. doi: 10.1038/s41592-018-0227-4. Link to article on publisher's site
Journal/Book/Conference Title
Nature methods
Related Resources
PubMed ID
30504865
Repository Citation
Thorsen, Rasmus O.; Hulleman, Christiaan N.; Hammer, Mathias; Grünwald, David; Stallinga, Sjoerd; and Rieger, Bernd, "Impact of optical aberrations on axial position determination by photometry" (2018). RNA Therapeutics Institute Publications. 58.
https://escholarship.umassmed.edu/rti_pubs/58