RNA Therapeutics Institute Publications

Title

ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals

UMMS Affiliation

RNA Therapeutics Institute; Program in Bioinformatics and Integrative Biology

Publication Date

2018-05-17

Document Type

Article

Disciplines

Amino Acids, Peptides, and Proteins | Biochemistry, Biophysics, and Structural Biology | Cell and Developmental Biology | Genetic Phenomena | Genetics and Genomics | Nucleic Acids, Nucleotides, and Nucleosides

Abstract

Animal cells have a remarkable capacity to adopt durable and heritable gene expression programs or epigenetic states that define the physical properties and diversity of somatic cell types. The maintenance of epigenetic programs depends on poorly understood pathways that prevent gain or loss of inherited signals. In the germline, epigenetic factors are enriched in liquid-like perinuclear condensates called nuage. Here, we identify the deeply conserved helicase-domain protein, ZNFX-1, as an epigenetic regulator and component of nuage that interacts with Argonaute systems to balance epigenetic inheritance. Our findings suggest that ZNFX-1 promotes the 3' recruitment of machinery that propagates the small RNA epigenetic signal and thus counteracts a tendency for Argonaute targeting to shift 5' along the mRNA. These functional insights support the idea that recently identified subdomains of nuage, including ZNFX-1 granules or "Z-granules," may define spatial and temporal zones of molecular activity during epigenetic regulation.

Keywords

Argonaute, C. elegans, CSR-1, P-granule, PIWI, RNAa, RNAe, RdRP, ZNFX-1, nuage

DOI of Published Version

10.1016/j.molcel.2018.04.009

Source

Mol Cell. 2018 May 17;70(4):639-649.e6. doi: 10.1016/j.molcel.2018.04.009. Link to article on publisher's site

Journal/Book/Conference Title

Molecular cell

Related Resources

Link to Article in PubMed

PubMed ID

29775580

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