RNA Therapeutics Institute Publications
Title
Mechanism of Inhibition of Translation Termination by Blasticidin S
UMMS Affiliation
RNA Therapeutics Institute
Publication Date
2018-03-02
Document Type
Article
Disciplines
Biochemistry, Biophysics, and Structural Biology
Abstract
Understanding the mechanisms of inhibitors of translation termination may inform development of new antibacterials and therapeutics for premature termination diseases. We report the crystal structure of the potent termination inhibitor blasticidin S bound to the ribosomal 70S*release factor 1 (RF1) termination complex. Blasticidin S shifts the catalytic domain 3 of RF1 and restructures the peptidyl transferase center. Universally conserved uridine 2585 in the peptidyl transferase center occludes the catalytic backbone of the GGQ motif of RF1, explaining the structural mechanism of inhibition. Rearrangement of domain 3 relative to the codon-recognition domain 2 provides insight into the dynamics of RF1 implicated in termination accuracy.
Keywords
RF1, antibiotic, blasticidin S, stop-codon recognition, termination suppression
DOI of Published Version
10.1016/j.jmb.2018.01.007
Source
J Mol Biol. 2018 Mar 2;430(5):591-593. doi: 10.1016/j.jmb.2018.01.007. Link to article on publisher's site
Journal/Book/Conference Title
Journal of molecular biology
Related Resources
PubMed ID
29366636
Repository Citation
Svidritskiy, Egor and Korostelev, Andrei A., "Mechanism of Inhibition of Translation Termination by Blasticidin S" (2018). RNA Therapeutics Institute Publications. 49.
https://escholarship.umassmed.edu/rti_pubs/49