RNA Therapeutics Institute Publications

Title

Inhibition of CRISPR-Cas systems by mobile genetic elements

UMMS Affiliation

RNA Therapeutics Institute

Publication Date

2017-06-01

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Immunity | Microbiology | Therapeutics

Abstract

Clustered, regularly interspaced, short, palindromic repeats (CRISPR) loci, together with their CRISPR-associated (Cas) proteins, provide bacteria and archaea with adaptive immunity against invasion by bacteriophages, plasmids, and other mobile genetic elements. These host defenses impart selective pressure on phages and mobile elements to evolve countermeasures against CRISPR immunity. As a consequence of this pressure, phages and mobile elements have evolved 'anti-CRISPR' proteins that function as direct inhibitors of diverse CRISPR-Cas effector complexes. Some of these CRISPR-Cas complexes can be deployed as genome engineering platforms, and anti-CRISPRs could therefore be useful in exerting spatial, temporal, or conditional control over genome editing and related applications. Here we describe the discovery of anti-CRISPRs, the range of CRISPR-Cas systems that they inhibit, their mechanisms of action, and their potential utility in biotechnology.

DOI of Published Version

10.1016/j.mib.2017.06.003

Source

Curr Opin Microbiol. 2017 Jun;37:120-127. doi: 10.1016/j.mib.2017.06.003. Epub 2017 Jun 29. Link to article on publisher's site

Journal/Book/Conference Title

Current opinion in microbiology

Related Resources

Link to Article in PubMed

PubMed ID

28668720

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