Inhibition of CRISPR-Cas systems by mobile genetic elements
RNA Therapeutics Institute
Biochemistry, Biophysics, and Structural Biology | Genetics and Genomics | Immunity | Microbiology | Therapeutics
Clustered, regularly interspaced, short, palindromic repeats (CRISPR) loci, together with their CRISPR-associated (Cas) proteins, provide bacteria and archaea with adaptive immunity against invasion by bacteriophages, plasmids, and other mobile genetic elements. These host defenses impart selective pressure on phages and mobile elements to evolve countermeasures against CRISPR immunity. As a consequence of this pressure, phages and mobile elements have evolved 'anti-CRISPR' proteins that function as direct inhibitors of diverse CRISPR-Cas effector complexes. Some of these CRISPR-Cas complexes can be deployed as genome engineering platforms, and anti-CRISPRs could therefore be useful in exerting spatial, temporal, or conditional control over genome editing and related applications. Here we describe the discovery of anti-CRISPRs, the range of CRISPR-Cas systems that they inhibit, their mechanisms of action, and their potential utility in biotechnology.
DOI of Published Version
Curr Opin Microbiol. 2017 Jun;37:120-127. doi: 10.1016/j.mib.2017.06.003. Epub 2017 Jun 29. Link to article on publisher's site
Current opinion in microbiology
Sontheimer EJ, Davidson AR. (2017). Inhibition of CRISPR-Cas systems by mobile genetic elements. RNA Therapeutics Institute Publications. https://doi.org/10.1016/j.mib.2017.06.003. Retrieved from https://escholarship.umassmed.edu/rti_pubs/39