RNA Therapeutics Institute Publications

Title

Chitosan-Mangafodipir nanoparticles designed for intranasal delivery of siRNA and DNA to brain

UMMS Affiliation

RNA Therapeutics Institute; Department of Medicine

Publication Date

2-1-2018

Document Type

Article

Disciplines

Genetics and Genomics | Nanomedicine | Neuroscience and Neurobiology | Therapeutics

Abstract

The overall objective of the present research was to develop a nanocarrier system for non-invasive delivery to brain of molecules useful for gene therapy. Manganese-containing nanoparticles (mNPs) carrying anti-eGFP siRNA were tested in cell cultures of eGFP-expressing cell line of mouse fibroblasts (NIH3T3). The optimal mNPs were then tested in vivo in mice. Following intranasal instillation, mNPs were visualized by 7T MRI throughout brain at 24 and 48 hrs. mNPs were effective in significantly reducing GFP mRNA expression in Tg GFP+ mice in olfactory bulb, striatum, hippocampus and cortex. Intranasal instillation of mNPS loaded with dsDNA encoding RFP also resulted in expression of the RFP in multiple brain regions. In conclusion, mNPs carrying siRNA, or dsDNA were capable of delivering the payload from nose to brain. This approach for delivery of gene therapies to humans, if successful, will have a significant impact on disease-modifying therapeutics of neurodegenerative diseases.

Keywords

Gene silencing, Intranasal route, Nanocarrier, Small animal MRI

DOI of Published Version

10.1016/j.jddst.2017.11.013

Source

J Drug Deliv Sci Technol. 2018 Feb;43:453-460. doi: 10.1016/j.jddst.2017.11.013. Epub 2017 Nov 21. Link to article on publisher's site

Journal/Book/Conference Title

Journal of drug delivery science and technology

Related Resources

Link to Article in PubMed

PubMed ID

29805475

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