RNA Therapeutics Institute Publications
UMMS Affiliation
Department Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Publication Date
2015-12-21
Document Type
Article
Disciplines
Biochemistry, Biophysics, and Structural Biology | Cell and Developmental Biology | Cell Biology | Genetics and Genomics | Therapeutics
Abstract
Many messenger RNA export proteins have been identified; yet the spatial and temporal activities of these proteins and how they determine directionality of messenger ribonucleoprotein (mRNP) complex export from the nucleus remain largely undefined. Here, the bacteriophage PP7 RNA-labeling system was used in Saccharomyces cerevisiae to follow single-particle mRNP export events with high spatial precision and temporal resolution. These data reveal that mRNP export, consisting of nuclear docking, transport, and cytoplasmic release from a nuclear pore complex (NPC), is fast ( approximately 200 ms) and that upon arrival in the cytoplasm, mRNPs are frequently confined near the nuclear envelope. Mex67p functions as the principal mRNP export receptor in budding yeast. In a mex67-5 mutant, delayed cytoplasmic release from NPCs and retrograde transport of mRNPs was observed. This proves an essential role for Mex67p in cytoplasmic mRNP release and directionality of transport.
Keywords
RNA, mRNA, messenger RNA, yeast, Saccharomyces cerevisiae, Mex67p, messenger ribonucleoprotein, mRNP
Rights and Permissions
© 2015 Smith et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
DOI of Published Version
10.1083/jcb.201503135
Source
J Cell Biol. 2015 Dec 21;211(6):1121-30. doi: 10.1083/jcb.201503135. Link to article on publisher's site
Journal/Book/Conference Title
The Journal of cell biology
Related Resources
PubMed ID
26694837
Repository Citation
Smith, Carlas; Lari, Azra; Derrer, Carina Patrizia.; Ouwehand, Anette; Rossouw, Ammeret; Huisman, Maximiliaan; Dange, Thomas; Hopman, Mark; Joseph, Aviva; Zenklusen, Daniel; Weis, Karsten; Grünwald, David; and Montpetit, Ben, "In vivo single-particle imaging of nuclear mRNA export in budding yeast demonstrates an essential role for Mex67p" (2015). RNA Therapeutics Institute Publications. 19.
https://escholarship.umassmed.edu/rti_pubs/19
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Cell Biology Commons, Genetics and Genomics Commons, Therapeutics Commons