Genomic Amplifications Cause False Positives in CRISPR Screens
RNA Therapeutics Institute; Program in Molecular Medicine; Department of Molecular, Cell and Cancer Biology
Biochemistry, Biophysics, and Structural Biology | Cancer Biology | Cell and Developmental Biology | Genetics and Genomics | Therapeutics
In CRISPR-based screens for essential genes, Munoz and colleagues and Aguirre and colleagues show that gene-independent targeting of genomic amplifications in human cancer cell lines reduces proliferation or survival. The correlation between CRISPR target site copy number and lethality demonstrates the need for scrutiny and complementary approaches to rule out off-target effects and false positives in CRISPR screens.
DOI of Published Version
Cancer Discov. 2016 Aug;6(8):824-6. doi: 10.1158/2159-8290.CD-16-0665. Link to article on publisher's site
Sheel, Ankur and Xue, Wen, "Genomic Amplifications Cause False Positives in CRISPR Screens" (2016). RNA Therapeutics Institute Publications. 14.