RNA Therapeutics Institute Publications

Title

Genomic Amplifications Cause False Positives in CRISPR Screens

UMMS Affiliation

RNA Therapeutics Institute; Program in Molecular Medicine; Department of Molecular, Cell and Cancer Biology

Publication Date

8-1-2016

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Cancer Biology | Cell and Developmental Biology | Genetics and Genomics | Therapeutics

Abstract

In CRISPR-based screens for essential genes, Munoz and colleagues and Aguirre and colleagues show that gene-independent targeting of genomic amplifications in human cancer cell lines reduces proliferation or survival. The correlation between CRISPR target site copy number and lethality demonstrates the need for scrutiny and complementary approaches to rule out off-target effects and false positives in CRISPR screens.

DOI of Published Version

10.1158/2159-8290.CD-16-0665

Source

Cancer Discov. 2016 Aug;6(8):824-6. doi: 10.1158/2159-8290.CD-16-0665. Link to article on publisher's site

Journal/Book/Conference Title

Cancer discovery

Related Resources

Link to Article in PubMed

PubMed ID

27485003

Share

COinS