RNA Therapeutics Institute Publications
Title
Genomic Amplifications Cause False Positives in CRISPR Screens
UMMS Affiliation
RNA Therapeutics Institute; Program in Molecular Medicine; Department of Molecular, Cell and Cancer Biology
Publication Date
2016-08-01
Document Type
Article
Disciplines
Biochemistry, Biophysics, and Structural Biology | Cancer Biology | Cell and Developmental Biology | Genetics and Genomics | Therapeutics
Abstract
In CRISPR-based screens for essential genes, Munoz and colleagues and Aguirre and colleagues show that gene-independent targeting of genomic amplifications in human cancer cell lines reduces proliferation or survival. The correlation between CRISPR target site copy number and lethality demonstrates the need for scrutiny and complementary approaches to rule out off-target effects and false positives in CRISPR screens.
DOI of Published Version
10.1158/2159-8290.CD-16-0665
Source
Cancer Discov. 2016 Aug;6(8):824-6. doi: 10.1158/2159-8290.CD-16-0665. Link to article on publisher's site
Journal/Book/Conference Title
Cancer discovery
Related Resources
PubMed ID
27485003
Repository Citation
Sheel, Ankur and Xue, Wen, "Genomic Amplifications Cause False Positives in CRISPR Screens" (2016). RNA Therapeutics Institute Publications. 14.
https://escholarship.umassmed.edu/rti_pubs/14