RNA Therapeutics Institute Publications

 
The RNA Therapeutics Institute (RTI) at the University of Massachusetts Medical School was founded in 2009 and became an academic department in 2016, chaired by Phillip D. Zamore, Ph.D. The RTI is dedicated to leveraging the strong RNA biology and clinical research communities at UMMS to develop novel therapies for which RNA is the therapeutic target or modality. The RNA Therapeutics Institute faculty are recognized as scientific trailblazers, and include a Nobel Laureate, a Gairdner Prize recipient, the 2018 RNA Society early and mid-career award recipients, and two Howard Hughes Medical Institute Investigators. This collection showcases journal articles and other publications authored by RTI faculty and researchers.

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Publications from 2022

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Imaging Net Retrograde Axonal Transport In Vivo: A Physiological Biomarker, Pin-Tsun Justin Lee, Zachary C. Kennedy, Yuzhen Wang, Yimeng Lu, Carolina Cefaliello, Ozgun Uyan, Chun-Qing Song, Bruno M. D. C. Godinho, Zuoshang Xu, Mary Rusckowski, Wen Xue, and Robert H. Brown Jr.

Publications from 2021

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Micro-Meta App: an interactive software tool to facilitate the collection of microscopy metadata based on community-driven specifications [preprint], Alessandro Rigano, Mathias Hammer, Karl D. Bellve, Kevin E. Fogarty, David Grunwald, and Caterina Strambio-De-Castillia

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Biochemical Mechanism of Gene Expression Silencing by piRNA-directed PIWI-Clade Argonautes, Amena Arif

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The tiny, conserved zinc-finger protein GTSF1 helps PIWI proteins achieve their full catalytic potential [preprint], Amena Arif, Deniz M. Ozata, Cecilia Anderson, Natsuko Izumi, Yukihide Tomari, and Phillip D. Zamore

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Dietary suppression of MHC class II expression in intestinal epithelial cells enhances intestinal tumorigenesis, Semir Beyaz, Charlie Chung, Haiwei Mou, Khristian E. Bauer-Rowe, Michael E. Xifaras, Ilgin Ergin, Lenka Dohnalova, Moshe Biton, Karthik Shekhar, Onur Eskiocak, Katherine Papciak, Kadir Ozler, Mohammad Almeqdadi, Brian Yueh, Miriam Fein, Damodaran Annamalai, Eider Valle-Encinas, Aysegul Erdemir, Karoline Dogum, Vyom Shah, Aybuke Alici-Garipcan, Hannah V. Meyer, Deniz M. Ozata, Eran Elinav, Alper Kucukural, Pawan Kumar, Jeremy P. McAleer, James G. Fox, Christoph A. Thaiss, Aviv Regev, Jatin Roper, Stuart H. Orkin, and Omer H. Yilmaz

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Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP [preprint], Christine E. Carbone; Anna B. Loveland; Howard Gamper, Jr.; Ya-Ming Hou; Gabriel Demo; and Andrei A. Korostelev

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Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation, Gabriel Demo, Howard B. Gamper, Anna B. Loveland, Isao Masuda, Christine E. Carbone, Egor Svidritskiy, Ya-Ming Hou, and Andrei A. Korostelev

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Modulating ApoE with Tissue Specific siRNAs in Alzheimer’s Disease, Chantal M. Ferguson

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Comparative route of administration studies using therapeutic siRNAs show widespread gene modulation in Dorset sheep, Chantal M. Ferguson, Bruno M. D. C. Godinho, Julia F. Alterman, Andrew H. Coles, Matthew R. Hassler, Dimas Echeverria, James W. Gilbert, Emily G. Knox, Jillian Caiazzi, Reka A. Haraszti, Robert M. King, Toloo Taghian, Ajit S. Puri, Richard P. Moser, Matthew J. Gounis, Neil Aronin, Heather L. Gray-Edwards, and Anastasia Khvorova

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Widespread occurrence of hybrid internal-terminal exons in human transcriptomes [preprint], Ana Fiszbein, Michael McGurk, Ezequiel Calvo-Roitberg, GyeungYun Kim, Christopher B. Burge, and Athma A. Pai

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An Exploration of the Properties of Repair Template DNA that Promote Precision Genome Editing, Krishna S. Ghanta

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An Investigation into the Potential of Targeting Escherichia coli rne mRNA with Locked Nucleic Acid (LNA) Gapmers as an Antibacterial Strategy, Layla R. Goddard, Charlotte E. Mardle, Hassan Gneid, Ciara G. Ball, Darren M. Gowers, Helen S. Atkins, Louise E. Butt, Jonathan K. Watts, Helen A. Vincent, and Anastasia J. Callaghan

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Towards community-driven metadata standards for light microscopy: tiered specifications extending the OME model [preprint], Mathias Hammer, Maximiliaan Huisman, Farzin Farzam, David Grünwald, and Caterina Strambio-De-Castillia

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Towards community-driven metadata standards for light microscopy: tiered specifications extending the OME model, Mathias Hammer, Maximiliaan Huisman, Alessandro Rigano, Farzin Farzam, David Grunwald, and Caterina Strambio-De-Castillia

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Cryo-TEM simulations of amorphous radiation-sensitive samples using multislice wave propagation [preprint], Benjamin A. Himes and Nikolaus Grigorieff

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A perspective on Microscopy Metadata: data provenance and quality control [preprint], Maximiliaan Huisman, Mathias Hammer, Alex Rigano, David Grunwald, and Caterina Strambio-De-Castillia

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Deletion and replacement of long genomic sequences using prime editing, Tingting Jiang, Xiao-Ou Zhang, Zhiping Weng, and Wen Xue

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Molecular structure and conformation of stereocilia tip-links elucidated by cryo-electron tomography [preprint], Johannes Elferich, Sarah Clark, Jingpeng Ge, Oregon Health & Science University, Aya Matsui, and Eric Gouaux

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PIE-1 SUMOylation promotes germline fates and piRNA-dependent silencing in C. elegans, Heesun Kim, Yue-He Ding, Shan Lu, Mei-Qing Zuo, Wendy Tan, Darryl Conte Jr., Meng-Qiu Dong, and Craig C. Mello

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HDAC1 SUMOylation promotes Argonaute-directed transcriptional silencing in C. elegans, Heesun Kim, Yue-He Ding, Gangming Zhang, Yong-Hong Yan, Darryl Conte Jr., Meng-Qiu Dong, and Craig C. Mello

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Bacterial processing of glucose modulates C. elegans lifespan and healthspan, Samuel F. Kingsley, Yonghak Seo, Calista Allen, Krishna S. Ghanta, Steven Finkel, and Heidi A. Tissenbaum

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Diversity and Similarity of Termination and Ribosome Rescue in Bacterial, Mitochondrial, and Cytoplasmic Translation, Andrei A. Korostelev

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Deep sequencing of pre-translational mRNPs reveals hidden flux through evolutionarily conserved alternative splicing nonsense-mediated decay pathways, Carrie Kovalak, Scott Donovan, Alicia A. Bicknell, Mihir Metkar, and Melissa J. Moore

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Gene therapy with AR isoform 2 rescues spinal and bulbar muscular atrophy phenotype by modulating AR transcriptional activity, Wooi F. Lim, Annabelle Biscans, Anastasia Khvorova, and Carlo Rinaldi

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Locating macromolecular assemblies in cells by 2D template matching with cisTEM, Bronwyn A. Lucas, Benjamin A. Himes, Liang Xue, Timothy Grant, Julia Mahamid, and Nikolaus Grigorieff

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Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM [preprint], Bronwyn Lucas, Benjamin A. Himes, Liang Xue, Timothy Grant, Julia Mahamid, and Nikolaus Grigorieff

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Quantifying and Mitigating Motor Phenotypes Induced by Antisense Oligonucleotides in the Central Nervous System [preprint], Michael P. Moazami, Julia M. Rembetsy-Brown, Feng Wang, Pranathi Meda Krishnamurthy, Alexandra Weiss, Miklos G. Marosfoi, Robert M. King, Mona Motwani, Heather L. Gray-Edwards, Katherine A. Fitzgerald, Robert H. Brown Jr., and Jonathan K. Watts

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Androgen Activity Is Associated With PD-L1 Downregulation in Thyroid Cancer, Timmy J. O'Connell, Sina Dadafarin, Melanie Jones, Tomás Rodríguez, Anvita Gupta, Edward Shin, Augustine Moscatello, Codrin Iacob, Humayun Islam, Raj K. Tiwari, and Jan Geliebter

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The testis-specific transcription factor TCFL5 responds to A-MYB to elaborate the male meiotic program in placental mammals [preprint], Deniz M. Ozata, Tianxiong Yu, Katharine Cecchini, Haiwei Mou, Amena Arif, Cansu Colpan, Adriano Biasini, Ildar Gaitendinov, Dirk G. de Rooij, Zhiping Weng, and Phillip D. Zamore

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Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice, Pengpeng Liu, Shun-Qing Liang, Chunwei Zheng, Esther Mintzer, Yan G. Zhao, Karthikeyan Ponnienselvan, Aamir Mir, Erik J. Sontheimer, Guangping Gao, Terence R. Flotte, Scot A. Wolfe, and Wen Xue

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Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications, Alessandro Rigano, Mathias Hammer, Karl D. Bellve, Kevin E. Fogarty, David Grunwald, and Caterina Strambio-De-Castillia

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The NIH Somatic Cell Genome Editing program, Krishanu Saha, Erik J. Sontheimer, Guangping Gao, Anastasia Khvorova, Jonathan K. Watts, and Scot A. Wolfe

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REMBI: Recommended Metadata for Biological Images-enabling reuse of microscopy data in biology, Ugis Sarkans, David Grunwald, Caterina Strambio-De-Castillia, Gerard J. Kleywegt, and Alvis Brazma

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Quantification of Antisense Oligonucleotides by Splint Ligation and Quantitative Polymerase Chain Reaction [preprint], Minwook Shin, Pranathi Meda Krishnamurthy, and Jonathan K. Watts

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Germ granule dysfunction is a hallmark and mirror of Piwi mutant sterility, Maya Spichal, Bree Heestand, Katherine Kretovich Billmyre, Stephen Frenk, Craig C. Mello, and Shawn Ahmed

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Suppression of mutant C9orf72 expression by a potent mixed backbone antisense oligonucleotide, Helene Tran, Michael P. Moazami, Huiya Yang, Diane Mckenna-Yasek, Catherine Douthwright, Courtney Pinto, Jake P. Metterville, Minwook Shin, Nitasha Sanil, Craig Dooley, Ajit S. Puri, Alexandra Weiss, Nicholas Wightman, Heather L. Gray-Edwards, Miklos G. Marosfoi, Robert M. King, Jonathan K. Watts, and Robert H. Brown Jr.

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A cohesin traffic pattern genetically linked to gene regulation [preprint], Anne-Laure Valton, Sergey V. Venev, Barbara Mair, Eraj Khokhar, Amy H. Y. Tong, Matej Usaj, Katherine S. K. Chan, Athma A. Pai, Jason Moffat, and Job Dekker

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Low-level overexpression of wild type TDP-43 causes late-onset, progressive neurodegeneration and paralysis in mice [preprint], Chunxing Yang, Tao Qiao, Jia Yu, Hongyan Wang, Yansu Guo, Johnny Salameh, Jake P. Metterville, Sepideh Parsi, Robert H. Brown Jr., Huaibin Cai, and Zuoshang Xu

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Long first exons and epigenetic marks distinguish conserved pachytene piRNA clusters from other mammalian genes, Tianxiong Yu, Kaili Fan, Deniz M. Ozata, Gen Zhang, Yu Fu, William E. Theurkauf, Phillip D. Zamore, and Zhiping Weng

Publications from 2020

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mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding, Chen Bao, Sarah Loerch, Clarence Ling, Andrei A. Korostelev, Nikolaus Grigorieff, and Dmitri N. Ermolenko

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mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding [preprint], Chen Bao, Sarah Loerch, Clarence Ling, Andrei A. Korostelev, Nikolaus Grigorieff, and Dmitri N. Ermolenko

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Ly6a Differential Expression in Blood-Brain Barrier Is Responsible for Strain Specific Central Nervous System Transduction Profile of AAV-PHP.B, Ana Rita Batista, Oliver D. King, Christopher P. Reardon, Crystal Davis, FNU Shankaracharya, Vivek Philip, Heather Gray-Edwards, Neil Aronin, Cathleen Lutz, John E. Landers, and Miguel Sena-Esteves

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Dietary suppression of MHC-II expression in intestinal stem cells enhances intestinal tumorigenesis [preprint], Semir Beyaz, Deniz M. Ozata, Alper Kucukural, Stuart H. Orkin, and Omer H. Yilmaz

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The chemical structure and phosphorothioate content of hydrophobically modified siRNAs impact extrahepatic distribution and efficacy, Annabelle Biscans, Jillian Caiazzi, Sarah M. Davis, Nicholas McHugh, Jacquelyn Sousa, and Anastasia Khvorova

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Docosanoic acid conjugation to siRNA enables functional and safe delivery to skeletal and cardiac muscles, Annabelle Biscans, Jillian Caiazzi, Nicholas McHugh, Vignesh Hariharan, Manish Muhuri, and Anastasia Khvorova

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ArfB can displace mRNA to rescue stalled ribosomes, Christine E. Carbone, Gabriel Demo, Rohini Madireddy, Egor Svidritskiy, and Andrei A. Korostelev

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A Cas9 with PAM recognition for adenine dinucleotides, Pranam Chatterjee, Jooyoung Lee, Lisa Nip, Sabrina R. T. Koseki, Emma Tysinger, Erik J. Sontheimer, Joseph M. Jacobson, and Noah Jakimo

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The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans [preprint], Daniel A. Chaves, Hui Dai, Lichao Li, James J. Moresco, Myung Eun Oh, Darryl Conte Jr., John R. Yates III, Craig C. Mello, and Weifeng Gu

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Understanding the Production and Stability of Mouse PIWI-Interacting RNAs, Cansu Colpan

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2'-O-Methyl at 20-mer Guide Strand 3' Termini May Negatively Affect Target Silencing Activity of Fully Chemically Modified siRNA, Sarah M. Davis, Jacquelyn Sousa, Lorenc Vangjeli, Matthew R. Hassler, Dimas Echeverria, Emily G. Knox, Anton A. Turanov, Julia F. Alterman, and Anastasia Khvorova

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Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation [preprint], Gabriel Demo, Anna B. Loveland, Egor Svidritskiy, Howard B. Gamper, Ya-Ming Hou, and Andrei A. Korostelev

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Cell Type Impacts Accessibility of mRNA to Silencing by RNA Interference, Chantal M. Ferguson, Dimas Echeverria, Matthew R. Hassler, Socheata Ly, and Anastasia Khvorova

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Melting dsDNA Donor Molecules Greatly Improves Precision Genome Editing in Caenorhabditis elegans, Krishna S. Ghanta and Craig C. Mello

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Melting dsDNA donor molecules potentiates precision genome editing in C. elegans [preprint], Krishna S. Ghanta and Craig C. Mello

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Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly, Shijie Huang, Nikolay A. Aleksashin, Anna B. Loveland, Dorota Klepacki, Kaspar Reier, Amira Kefi, Teresa Szal, Jaanus Remme, Luc Jaeger, Nora Vazquez-Laslop, Andrei A. Korostelev, and Alexander S. Mankin

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Genome Engineering Goes Viral: Repurposing of Adeno-associated Viral Vectors for CRISPR-mediated in Vivo Genome Engineering, Raed R. Ibraheim

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Precision Cas9 Genome Editing in vivo with All-in-one, Self-targeting AAV Vectors [preprint], Raed Ibraheim, Phillip W. L. Tai, Aamir Mir, Nida Javeed, Jiaming Wang, Tomas Rodriguez, Samantha J. Nelson, Eraj Khokhar, Esther Mintzer, Stacy Maitland​, Yueying Cao, Guangping Gao, and Erik J. Sontheimer

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Structure of the Vesicular Stomatitis Virus L Protein in Complex with Its Phosphoprotein Cofactor, Simon Jenni, Louis-Marie Bloyet, Ruben Diaz-Avalos, Bo Liang, Sean P.J. Whelan, Nikolaus Grigorieff, and Stephen C. Harrison

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Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope, Tingting Jiang, Xiao-Ou Zhang, Yueying Cao, Zhiping Weng, and Wen Xue

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Thermus thermophilus Argonaute Functions in the Completion of DNA Replication, Samson M. Jolly

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Thermus thermophilus Argonaute Functions in the Completion of DNA Replication, Samson M. Jolly, Ildar Gainetdinov, , Han Zhang, Lara Strittmatter, Shannon M. Bailey, Gregory M. Hendricks, Avantika Dhabaria, Beatrix Ueberheide, and Phillip D. Zamore

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Anti-CRISPR Proteins: Applications in Genome Engineering, Jooyoung Lee

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Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM [preprint], Anna B. Loveland, Egor Svidritskiy, Denis Susorov, Soojin Lee, Alexander Park, Gabriel Demo, Fen-Biao Gao, and Andrei A. Korostelev

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Investigating the Role of Mutant Huntingtin mRNA in Huntington’s Disease, Socheata Ly

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Single-Stranded Phosphorothioated Regions Enhance Cellular Uptake of Cholesterol-Conjugated siRNA but Not Silencing Efficacy, Socheata Ly, Dimas Echeverria, Jacquelyn Sousa, and Anastasia Khvorova

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Loss of huntingtin function slows synaptic vesicle endocytosis in striatal neurons from the htt(Q140/Q140) mouse model of Huntington's disease, Robyn L. McAdam, Andrew Morton, Sarah L. Gordon, Julia F. Alterman, Anastasia Khvorova, Michael A. Cousin, and Karen J. Smillie

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Translation-dependent and independent mRNA decay occur through mutually exclusive pathways that are defined by ribosome density during T Cell activation [preprint], Blandine C. Mercier, Emmanuel Labaronne, David Cluet, Alicia A. Bicknell, Antoine Corbin, Laura Guiguettaz, Fabien Aube, Laurent Modolo, Didier Auboeuf, Melissa J. Moore, and Emiliano P. Ricci

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LHRH-Conjugated Drugs as Targeted Therapeutic Agents for the Specific Targeting and Localized Treatment of Triple Negative Breast Cancer, J. D. Obayemi, A. A. Salifu, S. C. Eluu, V. O. Uzonwanne, S. M. Jusu, C. C. Nwazojie, C. E. Onyekanne, Ogooluwa Ojelabi, L. Payne, Constance M. Moore, Jean A. King, and W. O. Soboyejo

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Detecting chromatin interactions along and between sister chromatids with SisterC [preprint], Marlies E. Oomen, Adam K. Hedger, Jonathan K. Watts, and Job Dekker

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Evolutionarily conserved pachytene piRNA loci are highly divergent among modern humans, Deniz M. Ozata, Tianxiong Yu, Haiwei Mou, Ildar Gainetdinov, Cansu Colpan, Katharine Cecchini, Yasin Kaymaz, Pei-Hsuan Wu, Kaili Fan, Alper Kucukural, Zhiping Weng, and Phillip D. Zamore

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The evolutionarily conserved piRNA-producing locus pi6 is required for male mouse fertility, Pei-Hsuan Wu, Yu Fu, Katharine Cecchini, Deniz M. Ozata, Amena Arif, Tianxiong Yu, Cansu Colpan, Ildar Gainetdinov, Zhiping Weng, and Phillip D. Zamore

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Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice [preprint], Pengpeng Liu, Shun-Qing Liang, Chunwei Zheng, Esther Mintzer, Yan G. Zhao, Karthikeyan Ponnienselvan, Aamir Mir, Erik J. Sontheimer, Guangping Gao, Terence R. Flotte, Scot A. Wolfe, and Wen Xue

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Data on enrichment of chitosan nanoparticles for intranasal delivery of oligonucleotides to the brain, Vasyl Sava, Oksana Fihurka, Anastasia Khvorova, and Juan Sanchez-Ramos

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Reversing Cancer Cell Fate: Driving Therapeutic Differentiation of Hepatoblastoma to Functional Hepatocyte-Like Cells, Jordan L. Smith

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YAP1 withdrawal in hepatoblastoma drives therapeutic differentiation of tumor cells to functional hepatocyte-like cells, Jordan L. Smith, Tomas Rodriguez, Haiwei Mou, Suet-Yan Kwan, Henry E. Pratt, Xiao-Ou Zhang, Yueying Cao, Shun-Qing Liang, Deniz M. Ozata, Tianxiong Yu, Qiangzong Yin, Max Hazeltine, Zhiping Weng, Erik J. Sontheimer, and Wen Xue

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Adenine base editing in an adult mouse model of tyrosinaemia, Chun-Qing Song, Tingting Jiang, Michelle Richter, Luke H. Rhym, Luke W. Koblan, Maria Paz Zafra, Emma M. Schatoff, Jordan L. Doman, Yueying Cao, Lukas E. Dow, Lihua Julie Zhu, Daniel G. Anderson, David R. Liu, Hao Yin, and Wen Xue

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Termi-Luc: a versatile assay to monitor full-protein release from ribosomes, Denis Susorov, Shawn B. Egri, and Andrei A. Korostelev

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CRISPR-enhanced human adipocyte 'browning' as cell therapy for metabolic disease [preprint], Emmanouela Tsagkaraki, Sarah M. Nicoloro, Tiffany DeSouza, Javier Solivan-Rivera, Anand Desai, Yuefei Shen, Mark Kelly, Adilson L. Guilherme, Felipe Henriques, Raed Ibraheim, Nadia Amrani, Kevin Luk, Stacy Maitland, Randall H. Friedline, Lauren Tauer, Xiaodi Hu, Jason K. Kim, Scot A. Wolfe, Erik J. Sontheimer, Silvia Corvera, and Michael P. Czech

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DolphinNext: a distributed data processing platform for high throughput genomics, Onur Yukselen, Osman Turkyilmaz, Ahmet R. Ozturk, Manuel Garber, and Alper Kucukural

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Comprehensive identification of alternative back-splicing in human tissue transcriptomes, Peng Zhang, Xiao-Ou Zhang, Tingting Jiang, Lingling Cai, Xiao Huang, Qi Liu, Dan Li, Aiping Lu, Yan Liu, Wen Xue, Peng Zhang, and Zhiping Weng

Publications from 2019

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A CNS-Active siRNA Chemical Scaffold for the Treatment of Neurodegenerative Diseases, Julia F. Alterman

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A divalent siRNA chemical scaffold for potent and sustained modulation of gene expression throughout the central nervous system, Julia F. Alterman, Bruno M. D. C. Godinho, Matthew R. Hassler, Chantal M. Ferguson, Dimas Echeverria, Ellen Sapp, Reka A. Haraszti, Andrew H. Coles, Faith Conroy, Rachael Miller, Loic Roux, Paul Yan, Emily G. Knox, Anton A. Turanov, Robert M. King, Gwladys Gernoux, Christian Mueller, Heather Gray-Edwards, Richard P. Moser, N Bishop, Samer M. Jaber, Matthew J. Gounis, Miguel Sena-Esteves, Athma A. Pai, Marian DiFiglia, Neil Aronin, and Anastasia Khvorova

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Ninjurin1 positively regulates osteoclast development by enhancing the survival of prefusion osteoclasts, Sung-Jin Bae, Min Wook Shin, Taekwon Son, Hye Shin. Lee, Ji Soo Chae, Sejin Jeon, Goo Taeg Oh, and Kyu-Won Kim

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Diverse lipid conjugates for functional extra-hepatic siRNA delivery in vivo, Annabelle Biscans, Andrew H. Coles, Reka A. Haraszti, Dimas Echeverria, Matthew R. Hassler, Maire F. Osborn, and Anastasia Khvorova

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Compact Cas9s and Their Natural Inhibitors for Genome Editing, Alireza Edraki

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A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing, Alireza Edraki, Aamir Mir, Raed Ibraheim, Ildar Gainetdinov, Yeonsoo Yoon, Chun-Qing Song, Yueying Cao, Judith Gallant, Wen Xue, Jaime A. Rivera, and Erik J. Sontheimer

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Combining CRISPR-Cas9 and Proximity Labeling to Illuminate Chromatin Composition, Organization, and Regulation, Xin D. Gao

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Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing, Bianca Garcia, Jooyoung Lee, Alireza Edraki, Yurima Hidalgo-Reyes, Steven Erwood, Aamir Mir, Chantel N. Trost, Uri Seroussi, Sabrina Y. Stanley, Ronald D. Cohn, Julie M. Claycomb, Erik J. Sontheimer, Karen L. Maxwell, and Alan R. Davidson

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The RNA-Binding ATPase, Armitage, Couples piRNA Amplification in Nuage to Phased piRNA Production on Mitochondria, Daniel Tianfang Ge, Wei Wang, Cindy Tipping, Ildar Gainetdinov, Zhiping Weng, and Phillip D. Zamore

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Serum Deprivation of Mesenchymal Stem Cells Improves Exosome Activity and Alters Lipid and Protein Composition, Reka A. Haraszti, Rachael Miller, Michelle L. Dubuke, Andrew H. Coles, Marie C. Didiot, Dimas Echeverria, Matteo Stoppato, Yves Y. Sere, John D. Leszyk, Julia F. Alterman, Bruno M. D. C. Godinho, Matthew R. Hassler, Rachel Wollacott, Yan Wang, Scott A. Shaffer, Neil Aronin, and Anastasia Khvorova

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Vision Beyond Optics: Standardization, Evaluation and Innovation for Fluorescence Microscopy in Life Sciences, Maximiliaan Huisman

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In situ Structure of Rotavirus VP1 RNA-Dependent RNA Polymerase, Simon Jenni, Eric N. Salgado, Tobias Herrmann, Zongli Li, Timothy Grant, Nikolaus Grigorieff, Stefano Trapani, Leandro F. Estrozi, and Stephen C. Harrison

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A DNA-guided Argonaute Protein Functions in DNA Replication in Thermus thermophilus [preprint], Samson M. Jolly, Ildar Gainetdinov, , Han Zhang, Lara Strittmatter, Gregory M. Hendricks, Avantika Dhabaria, Beatrix Ueberheide, and Phillip D. Zamore

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High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations, James Jung, Timothy Grant, Dennis R. Thomas, Chris W. Diehnelt, Nikolaus Grigorieff, and Leemor Joshua-Tor

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Optimizing CRISPR/Cas9 for Gene Silencing of SOD1 in Mouse Models of ALS, Zachary C. Kennedy

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Deep sequencing of pre-translational mRNPs reveals hidden flux through evolutionarily conserved AS-NMD pathways [preprint], Carrie Kovalak, Mihir Metkar, and Melissa J. Moore

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DEBrowser: interactive differential expression analysis and visualization tool for count data, Alper Kucukural, Onur Yukselen, Deniz M. Ozata, Melissa J. Moore, and Manuel Garber

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Depletion of TRRAP induces p53-independent senescence in liver cancer by downregulating mitotic genes, Suet-Yan Kwan, Ankur Sheel, Chun-Qing Song, Xiao-Ou Zhang, Tingting Jiang, Hien Dang, Yueying Cao, Deniz M. Ozata, Haiwei Mou, Hao Yin, Zhiping Weng, Xin Wei Wang, and Wen Xue

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Tissue-restricted genome editing in vivo specified by microRNA-repressible anti-CRISPR proteins, Jooyoung Lee, Haiwei Mou, Raed Ibraheim, Shun-Qing Liang, Pengpeng Liu, Wen Xue, and Erik J. Sontheimer

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Tissue-specific Genome Editing in vivo by MicroRNA-repressible Anti-CRISPR Proteins [preprint], Jooyoung Lee, Haiwei Mou, Raed Ibraheim, Shun-Qing Liang, Wen Xue, and Erik J. Sontheimer