RNA Therapeutics Institute Publications

 
The RNA Therapeutics Institute (RTI) at the University of Massachusetts Medical School was founded in 2009 and became an academic department in 2016, chaired by Phillip D. Zamore, Ph.D. The RTI is dedicated to leveraging the strong RNA biology and clinical research communities at UMMS to develop novel therapies for which RNA is the therapeutic target or modality. The RNA Therapeutics Institute faculty are recognized as scientific trailblazers, and include a Nobel Laureate, a Gairdner Prize recipient, the 2018 RNA Society early and mid-career award recipients, and two Howard Hughes Medical Institute Investigators. This collection showcases journal articles and other publications authored by RTI faculty and researchers.

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Publications from 2019

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A CNS-Active siRNA Chemical Scaffold for the Treatment of Neurodegenerative Diseases, Julia F. Alterman

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Ninjurin1 positively regulates osteoclast development by enhancing the survival of prefusion osteoclasts, Sung-Jin Bae, Min Wook Shin, Taekwon Son, Hye Shin. Lee, Ji Soo Chae, Sejin Jeon, Goo Taeg Oh, and Kyu-Won Kim

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Diverse lipid conjugates for functional extra-hepatic siRNA delivery in vivo, Annabelle Biscans, Andrew H. Coles, Reka A. Haraszti, Dimas Echeverria, Matthew R. Hassler, Maire F. Osborn, and Anastasia Khvorova

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A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing, Alireza Edraki, Aamir Mir, Raed Ibraheim, Ildar Gainetdinov, Yeonsoo Yoon, Chun-Qing Song, Yueying Cao, Judith Gallant, Wen Xue, Jaime A. Rivera, and Erik J. Sontheimer

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The RNA-Binding ATPase, Armitage, Couples piRNA Amplification in Nuage to Phased piRNA Production on Mitochondria, Daniel Tianfang Ge, Wei Wang, Cindy Tipping, Ildar Gainetdinov, Zhiping Weng, and Phillip D. Zamore

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Serum Deprivation of Mesenchymal Stem Cells Improves Exosome Activity and Alters Lipid and Protein Composition, Reka A. Haraszti, Rachael Miller, Michelle L. Dubuke, Andrew H. Coles, Marie C. Didiot, Dimas Echeverria, Matteo Stoppato, Yves Y. Sere, John D. Leszyk, Julia F. Alterman, Bruno M. D. C. Godinho, Matthew R. Hassler, Rachel Wollacott, Yan Wang, Scott A. Shaffer, Neil Aronin, and Anastasia Khvorova

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Vision Beyond Optics: Standardization, Evaluation and Innovation for Fluorescence Microscopy in Life Sciences, Maximiliaan Huisman

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In situ Structure of Rotavirus VP1 RNA-Dependent RNA Polymerase, Simon Jenni, Eric N. Salgado, Tobias Herrmann, Zongli Li, Timothy Grant, Nikolaus Grigorieff, Stefano Trapani, Leandro F. Estrozi, and Stephen C. Harrison

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High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations, James Jung, Timothy Grant, Dennis R. Thomas, Chris W. Diehnelt, Nikolaus Grigorieff, and Leemor Joshua-Tor

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Optimizing CRISPR/Cas9 for Gene Silencing of SOD1 in Mouse Models of ALS, Zachary C. Kennedy

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DEBrowser: interactive differential expression analysis and visualization tool for count data, Alper Kucukural, Onur Yukselen, Deniz M. Ozata, Melissa J. Moore, and Manuel Garber

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Depletion of TRRAP induces p53-independent senescence in liver cancer by downregulating mitotic genes, Suet-Yan Kwan, Ankur Sheel, Chun-Qing Song, Xiao-Ou Zhang, Tingting Jiang, Hien Dang, Yueying Cao, Deniz M. Ozata, Haiwei Mou, Hao Yin, Zhiping Weng, Xin Wei Wang, and Wen Xue

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CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling, Haiwei Mou, Deniz M. Ozata, Jordan L. Smith, Ankur Sheel, Suet-Yan Kwan, Soren Hough, Alper Kucukural, Zachary Kennedy, Yueying Cao, and Wen Xue

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Hydrophobicity drives the systemic distribution of lipid-conjugated siRNAs via lipid transport pathways, Maire F. Osborn, Andrew H. Coles, Annabelle Biscans, Reka A. Haraszti, Loic Roux, Sarah M. Davis, Socheata Ly, Dimas Echeverria, Matthew R. Hassler, Bruno M. D. C. Godinho, Mehran Nikan, and Anastasia Khvorova

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Intron and Small RNA Localization in Mammalian Neurons, Harleen Saini

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Identification of Essential Genes in Hepatocellular Carcinomas using CRISPR Screening, Ankur Sheel

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An automated Bayesian pipeline for rapid analysis of single-molecule binding data, Carlas Smith, Karina Jouravleva, Maximiliaan Huisman, Samson M. Jolly, Phillip D. Zamore, and David Grünwald

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Principles for enhancing virus capsid capacity and stability from a thermophilic virus capsid structure, Nicholas P. Stone, Gabriel Demo, Emily Agnello, and Brian A. Kelch

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Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2, Annoj Thavalingam, Erik J. Sontheimer, Yanli Wang, and Karen L. Maxwell

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Gene Silencing With siRNA (RNA Interference): A New Therapeutic Option During Ex Vivo Machine Liver Perfusion Preservation, Max F. Thijssen, Isabel M. A. Bruggenwirth, Andrew R. Gillooly, Anastasia Khvorova, Timothy F. Kowalik, and Paulo N.A. Martins

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Rac1 Activity Is Modulated by Huntingtin and Dysregulated in Models of Huntington's Disease, Adelaide Tousley, Anastasia Khvorova, Neil Aronin, and Kimberly B. Kegel-Gleason

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Targeted Metabolomics Identifies the Cytochrome P450 Monooxygenase Eicosanoid Pathway as a Novel Therapeutic Target of Colon Tumorigenesis, Weicang Wang, Jun Yang, Matthew L. Edin, Yuxin Wang, Ying Luo, Debin Wan, Haixia Yang, Chun-Qing Song, Wen Xue, Katherine Z. Sanidad, Mingyue Song, Heather A. Bisbee, Jennifer A. Bradbury, Guanjun Nan, Jianan Zhang, Pei-An Betty Shih, Kin Sing Stephen Lee, Lisa M. Minter, Daeyoung Kim, Hang Xiao, Jun-Yan Liu, Bruce D. Hammock, Darryl C. Zeldin, and Guodong Zhang

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IIV-6 Inhibits NF-kappaB Responses in Drosophila, Cara C. West, Florentina Rus, Ying Chen, Anni Kleino, Monique Gangloff, Don B. Gammon, and Neal S. Silverman

Publications from 2018

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Bayesian nonparametric discovery of isoforms and individual specific quantification, Derek Aguiar, Li-Fang Cheng, Bianca Dumitrascu, Fantine Mordelet, Athma A. Pai, and Barbara E. Engelhardt

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NmeCas9 is an intrinsically high-fidelity genome editing platform, Nadia Amrani, Xin D. Gao, Pengpeng Liu, Alireza Edraki, Aamir Mir, Raed Ibraheim, Ankit Gupta, Kanae E. Sasaki, Tong Wu, Thomas G. Fazzio, Lihua Julie Zhu, Scot A. Wolfe, and Erik J. Sontheimer

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NmeCas9 is an intrinsically high-fidelity genome-editing platform, Nadia Amrani, Xin D. Gao, Pengpeng Liu, Alireza Edraki, Aamir Mir, Raed Ibraheim, Ankit Gupta, Kanae E. Sasaki, Tong Wu, Thomas G. Fazzio, Lihua Julie Zhu, Scot A. Wolfe, and Erik J. Sontheimer

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Exploring the Emergence of RNA Nucleosides and Nucleotides on the Early Earth, Annabelle Biscans

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Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing, Mehmet F. Bolukbasi, Pengpeng Liu, Kevin Luk, Samantha F. Kwok, Ankit Gupta, Nadia Amrani, Erik J. Sontheimer, Lihua Julie Zhu, and Scot A. Wolfe

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Synergistic assembly of human pre-spliceosomes across introns and exons, Joerg E. Braun, Larry J. Friedman, Jeff Gelles, and Melissa J. Moore

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Maelstrom Represses Canonical RNA Polymerase II Transcription in Drosophila Dual-Strand piRNA Clusters, Timothy H. Chang

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Maelstrom Represses Canonical Polymerase II Transcription within Bi-directional piRNA Clusters in Drosophila melanogaster, Timothy Chang, Eugenio Mattei, Ildar Gainetdinov, Cansu Colpan, Zhiping Weng, and Phillip D. Zamore

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Transcriptome-wide Interrogation of the Functional Intronome by Spliceosome Profiling, Weijun Chen, Jill E. Moore, Hakan Ozadam, Hennady P. Shulha, Nicholas Rhind, Zhiping Weng, and Melissa J. Moore

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Nuclear Localization of Huntingtin mRNA Is Specific to Cells of Neuronal Origin, Marie C. Didiot, Chantal M. Ferguson, Socheata Ly, Andrew H. Coles, Abigail O. Smith, Alicia A. Bicknell, Lauren M. Hall, Ellen Sapp, Dimas Echeverria, Athma A. Pai, Marian DiFiglia, Melissa J. Moore, Lawrence J. Hayward, Neil Aronin, and Anastasia Khvorova

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Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans, Gregoriy A. Dokshin, Krishna S. Ghanta, Katherine M. Piscopo, and Craig C. Mello

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Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers, Yu Fu, Pei-Hsuan Wu, Timothy J. Beane, Phillip D. Zamore, and Zhiping Weng

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Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers, Yu Fu, Pei-Hsuan Wu, Timothy J. Beane, Phillip D. Zamore, and Zhiping Weng

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The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology, Yu Fu, Yujing Yang, Han Zhang, Gwen Farley, Junling Wang, Kaycee A. Quarles, Zhiping Weng, and Phillip D. Zamore

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C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2, Xin D. Gao, Li-Chun Tu, Aamir Mir, Tomas Rodriguez, Yue-He Ding, John D. Leszyk, Job Dekker, Scott A. Shaffer, Lihua Julie Zhu, Scot A. Wolfe, and Erik J. Sontheimer (Nature methods, 2018-05-07)

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C-BERST: Defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2, Xin D. Gao, Li-Chun Tu, Aamir Mir, Tomas Rodriguez, Yue-He Ding, John D. Leszyk, Job Dekker, Scott A. Shaffer, Lihua Julie Zhu, Scot A. Wolfe, and Erik J. Sontheimer

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Understanding Nuage-mitochondrial Coupling in Drosophila piRNA Biogenesis, Tianfang Ge

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Engineered Exosomes for Delivery of Therapeutic siRNAs to Neurons, Reka A. Haraszti

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Optimized Cholesterol-siRNA Chemistry Improves Productive Loading onto Extracellular Vesicles, Reka A. Haraszti, Rachael Miller, Marie C. Didiot, Annabelle Biscans, Julia F. Alterman, Matthew R. Hassler, Loic Roux, Dimas Echeverria, Ellen Sapp, Marian DiFiglia, Neil Aronin, and Anastasia Khvorova

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Exosomes Produced from 3D Cultures of MSCs by Tangential Flow Filtration Show Higher Yield and Improved Activity, Reka A. Haraszti, Rachael Miller, Matteo Stoppato, Yves Y. Sere, Andrew H. Coles, Marie-Claire Daou, Rachel Wollacott, Ellen Sapp, Michelle L. Dubuke, Xuni Li, Scott A. Shaffer, Marian DiFiglia, Yan Wang, Neil Aronin, and Anastasia Khvorova

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Comparison of partially and fully chemically-modified siRNA in conjugate-mediated delivery in vivo, Matthew R. Hassler, Anton A. Turanov, Julia F. Alterman, Reka A. Haraszti, Andrew H. Coles, Maire F. Osborn, Dimas Echeverria, Mehran Nikan, William E. Salomon, Loic Roux, Bruno M. D. C. Godinho, Sarah M. Davis, David V. Morrissey, Phillip D. Zamore, S. Ananth Karumanchi, Melissa J. Moore, Neil Aronin, and Anastasia Khvorova

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Oligonucleotides targeting TCF4 triplet repeat expansion inhibit RNA foci and mis-splicing in Fuchs' dystrophy, Jiaxin Hu, Ziye Rong, Xin Gong, Zhengyang Zhou, Vivek K. Sharma, Chao Xing, Jonathan K. Watts, David R. Corey, and V. Vinod Mootha

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All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo, Raed Ibraheim, Chun-Qing Song, Aamir Mir, Nadia Amrani, Wen Xue, and Erik J. Sontheimer

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All-in-One Adeno-associated Virus Delivery and Genome Editing by Neisseria meningitidis Cas9 in vivo, Raed Ibraheim, Chun-Qing Song, Aamir Mir, Nadia Amrani, Wen Xue, and Erik J. Sontheimer

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ZNFX-1 Functions within Perinuclear Nuage to Balance Epigenetic Signals, Takao Ishidate, Ahmet R. Ozturk, Daniel J. Durning, Rita Sharma, En-Zhi Shen, Hao Chen, Meetu Seth, Masaki Shirayama, and Craig C. Mello

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PIE-1, SUMOylation, and Epigenetic Regulation of Germline Specification in Caenorhabditis elegans, Heesun Kim

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Potent Cas9 Inhibition in Bacterial and Human Cells by AcrIIC4 and AcrIIC5 Anti-CRISPR Proteins, Jooyoung Lee, Aamir Mir, Alireza Edraki, Bianca Garcia, Nadia Amrani, Hannah E. Lou, Ildar Gainetdinov, April Pawluk, Raed Ibraheim, Xin D. Gao, Pengpeng Liu, Alan R. Davidson, Karen L. Maxwell, and Erik J. Sontheimer

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Pan-arthropod analysis reveals somatic piRNAs as an ancestral defence against transposable elements, Samuel H. Lewis, Yujing Yang, Phillip D. Zamore, and Francis M. Jiggins

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Structural dynamics of protein S1 on the 70S ribosome visualized by ensemble cryo-EM, Anna B. Loveland and Andrei A. Korostelev

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Transcriptome Profiling of Neovascularized Corneas Reveals miR-204 as a Multi-target Biotherapy Deliverable by rAAVs, Yi Lu, Phillip W. L. Tai, Jianzhong Ai, Dominic J. Gessler, Qin Su, Xieyi Yao, Qiang Zheng, Phillip D. Zamore, Xun Xu, and Guangping Gao

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RIPPLiT and ChimeraTie: High throughput tools for understanding higher order RNP structures, Mihir Metkar

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Higher-Order Organization Principles of Pre-translational mRNPs, Mihir Metkar, Hakan Ozadam, Bryan R. Lajoie, Maxim Imakaev, Leonid A. Mirny, Job Dekker, and Melissa J. Moore

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Higher-Order Organization Principles of Pre-translational mRNPs, Mihir Metkar, Hakan Ozadam, Bryan R. Lajoie, Maxim Imakaev, Leonid A. Mirny, Job Dekker, and Melissa J. Moore (Molecular cell, 2018-11-15)

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Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing, Aamir Mir, Julia F. Alterman, Matthew R. Hassler, Alexandre J. Debacker, Edward Hudgens, Dimas Echeverria, Michael H. Brodsky, Anastasia Khvorova, Jonathan K. Watts, and Erik J. Sontheimer

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Heavily and Fully Modified RNAs Guide Efficient SpyCas9-Mediated Genome Editing, Aamir Mir, Julia F. Alterman, Matthew R. Hassler, Alexandre J. Debacker, Edward Hudgens, Dimas Echeverria, Michael H. Brodsky, Anastasia Khvorova, Jonathan K. Watts, and Erik J. Sontheimer

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Type II-C CRISPR-Cas9 Biology, Mechanism, and Application, Aamir Mir, Alireza Edraki, Jooyoung Lee, and Erik J. Sontheimer

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Pof8 is a La-related protein and a constitutive component of telomerase in fission yeast, Diego J. Paez-Moscoso, Lili Pan, Rutendo F. Sigauke, Morgan R. Schroeder, Wen Tang, and Peter Baumann

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Numerous recursive sites contribute to accuracy of splicing of long introns in flies, Athma A. Pai, Joseph Paggi, Karen Adelman, and Christopher B. Burge

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Numerous recursive sites contribute to accuracy of splicing in long introns in flies, Athma A. Pai, Joseph M. Paggi, Paul Yan, Karen Adelman, and Christopher B. Burge

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A Fresh Look at Huntingtin mRNA Processing in Huntington's Disease, Lindsay S. Romo, Emily S. Mohn, and Neil Aronin

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Extracellular vesicles from mice with alcoholic liver disease carry a distinct protein cargo and induce macrophage activation through heat shock protein 90, Banishree Saha, Fatemeh Momen-Heravi, Istvan Furi, Karen Kodys, Donna Catalano, Anwesha Gangopadhyay, Reka A. Haraszti, Abhishek Satishchandran, Arvin Iracheta-Vellve, Adeyinka Adejumo, Scott A. Shaffer, and Gyongyi Szabo

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Chitosan-Mangafodipir nanoparticles designed for intranasal delivery of siRNA and DNA to brain, Juan Sanchez-Ramos, Shijie Song, Xiaoyuan Kong, Parastou Foroutan, Gary Martinez, William Dominguez-Viqueria, Shyam Mohapatra, Subhra Mohapatra, Reka A. Haraszti, Anastasia Khvorova, Neil Aronin, and Vasyl Sava

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The Coding Regions of Germline mRNAs Confer Sensitivity to Argonaute Regulation in C. elegans, Meetu Seth, Masaki Shirayama, Wen Tang, En-Zhi Shen, Shikui Tu, Heng-Chi Lee, Zhiping Weng, and Craig C. Mello

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Identification of piRNA Binding Sites Reveals the Argonaute Regulatory Landscape of the C. elegans Germline, En-Zhi Shen, Hao Chen, Ahmet R. Ozturk, Shikui Tu, Masaki Shirayama, Wen Tang, Yue-He Ding, Si-Yuan Dai, Zhiping Weng, and Craig C. Mello

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Functional features defining the efficacy of cholesterol-conjugated, self-deliverable, chemically modified siRNAs, Taisia Shmushkovich, Kathryn R. Monopoli, Diana Homsy, Dmitriy Leyfer, Monica Betancur-Boissel, Anastasia Khvorova, and Alexey D. Wolfson

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An Automated Bayesian Pipeline for Rapid Analysis of Single-Molecule Binding Data, Carlas Smith, Karina Jouravleva, Maximiliaan Huisman, Samson M. Jolly, Phillip D. Zamore, and David Grünwald

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Understanding and repurposing CRISPR-mediated alternative splicing, Jordan L. Smith, Haiwei Mou, and Wen Xue

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In vivo genome editing partially restores alpha1-antitrypsin in a murine model of AAT deficiency, Chun-Qing Song, Dan Wang, Tingting Jiang, Kevin O'Connor, Qiushi Tang, Lingling Cai, Xiangrui Li, Zhiping Weng, Hao Yin, Guangping Gao, Christian Mueller, Terence R. Flotte, and Wen Xue

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Editorial: Nucleic Acids Research and Nucleic Acid Therapeutics, Barry L. Stoddard, Anastasia Khvorova, David R. Corey, William S. Dynan, and Keith R. Fox

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Mechanism of premature translation termination on a sense codon, Egor Svidritskiy, Garbriel Demo, and Andrei A. Korostelev

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Mechanism of Inhibition of Translation Termination by Blasticidin S, Egor Svidritskiy and Andrei A. Korostelev

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A Sex Chromosome piRNA Promotes Robust Dosage Compensation and Sex Determination in C. elegans, Wen Tang, Meetu Seth, Shikui Tu, En-Zhi Shen, Qian Li, Masaki Shirayama, Zhiping Weng, and Craig C. Mello

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Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines, Clotilde Thery, Kenneth W. Witwer, Reka A. Haraszti, and Anastasia Khvorova

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Deconstructing transport-distribution reconstruction in the nuclear-pore complex, Li-Chun Tu, Maximiliaan Huisman, Yu-Chieh Chung, Carlas Smith, and David Grünwald

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RNAi modulation of placental sFLT1 for the treatment of preeclampsia, Anton A. Turanov, Matthew R. Hassler, Ami Ashar-Patel, Julia F. Alterman, Andrew H. Coles, Reka A. Haraszti, Loic Roux, Bruno M. D. C. Godinho, Dimas Echeverria, S. Ananth Karumanchi, Melissa J. Moore, and Anastasia Khvorova

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Cas9-mediated allelic exchange repairs compound heterozygous recessive mutations in mice, Dan Wang, Jia Li, Chun-Qing Song, Karen Tran, Haiwei Mou, Pei-Hsuan Wu, Phillip W. L. Tai L, Craig A. Mendonca, Lingzhi Ren, Blake Y. Wang, Qin Su, Dominic J. Gessler, Phillip D. Zamore, Wen Xue, and Guangping Gao

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Drug Resistance Mutation L76V Alters Nonpolar Interactions at the Flap-Core Interface of HIV-1 Protease, Andres Wong-Sam, Yuan-Fang Wang, Ying Zhang, Arun K. Ghosh, Robert W. Harrison, and Irene T. Weber

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Partial DNA-guided Cas9 enables genome editing with reduced off-target activity, Hao Yin, Chun-Qing Song, Sneha Suresh, Suet-Yan Kwan, Qiongqiong Wu, Stephen Walsh, Junmei Ding, Roman L. Bogorad, Lihua Julie Zhu, Scot A. Wolfe, Victor Koteliansky, Wen Xue, Robert Langer, and Daniel G. Anderson

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A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals, Phillip D. Zamore, Ildar Gainetdinov, Cansu Colpan, and Katharine Cecchini

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The temporal landscape of recursive splicing during Pol II transcription elongation in human cells, Xiao-Ou Zhang, Yu Fu, Haiwei Mou, Wen Xue, and Zhiping Weng

Publications from 2017

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A High-throughput Assay for mRNA Silencing in Primary Cortical Neurons in vitro with Oligonucleotide Therapeutics, Julia F. Alterman, Andrew H. Coles, Lauren M. Hall, Neil Aronin, Anastasia Khvorova, and Marie C. Didiot

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FLT1 and transcriptome-wide polyadenylation site (PAS) analysis in preeclampsia, Ami Ashar-Patel, Yasin Kaymaz, Augustine Rajakumar, Jeffrey A. Bailey, S. Ananth Karumanchi, and Melissa J. Moore

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A common class of transcripts with 5'-intron depletion, distinct early coding sequence features, and N1-methyladenosine modification, Can Cenik, Hon Nian Chua, Guramrit Singh, Abdalla Akef, Michael P. Snyder, Alexander F. Palazzo, Melissa J. Moore, and Frederick P. Roth

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Structure of RNA polymerase bound to ribosomal 30S subunit, Gabriel Demo, Aviram Rasouly, Nikita Vasilyev, Vladimir Svetlov, Anna B. Loveland, Ruben Diaz-Avalos, Nikolaus Grigorieff, Evgeny Nudler, and Andrei A. Korostelev

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Mechanism of ribosome rescue by ArfA and RF2, Gabriel Demo, Egor Svidritskiy, Rohini Madireddy, Ruben Diaz-Avalos, Timothy Grant, Nikolaus Grigorieff, Duncan Sousa, and Andrei A. Korostelev

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The Antiviral RNA Interference Response Provides Resistance to Lethal Arbovirus Infection and Vertical Transmission in Caenorhabditis elegans, Don B. Gammon, Takao Ishidate, Lichao Li, Weifeng Gu, Neal S. Silverman, and Craig C. Mello

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Protocol for analyzing protein ensemble structures from chemical cross-links using DynaXL, Zhou Gong, Zhu Liu, Xu Dong, Yue-He Ding, Meng-Qiu Dong, and Chun Tang

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Loading of Extracellular Vesicles with Chemically Stabilized Hydrophobic siRNAs for the Treatment of Disease in the Central Nervous System, Reka A. Haraszti, Andrew H. Coles, Neil Aronin, Anastasia Khvorova, and Marie C. Didiot

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5-Vinylphosphonate improves tissue accumulation and efficacy of conjugated siRNAs in vivo, Reka A. Haraszti, Loic Roux, Andrew H. Coles, Anton A. Turanov, Julia F. Alterman, Dimas Echeverria, Bruno M. D. C. Godinho, Neil Aronin, and Anastasia Khvorova

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A Broad-Spectrum Inhibitor of CRISPR-Cas9, Lucas B. Harrington, Kevin W. Doxzen, Alireza Edraki, Nadia Amrani, Erik J. Sontheimer, and Jennifer A. Doudna

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Fluorescence polarization control for on-off switching of single molecules at cryogenic temperatures, Christiaan Hulleman, Maximiliaan Huisman, Robert Moerland, David Grünwald, Sjoerd Stallinga, and Bernd Rieger

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The chemical evolution of oligonucleotide therapies of clinical utility, Anastasia Khvorova and Jonathan K. Watts

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Small methyltransferase RlmH assembles a composite active site to methylate a ribosomal pseudouridine, Cha San Koh, Rohini Madireddy, Timothy J. Beane, Phillip D. Zamore, and Andrei A. Korostelev

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Involvement of p38-betaTrCP-Tristetraprolin-TNFalpha axis in radiation pneumonitis, Pranathi Meda Krishnamurthy, Shirish Shukla, Paramita Ray, Rohit Mehra, Mukesh K. Nyati, Theodore S. Lawrence, and Dipankar Ray

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Identification of Factors Involved in 18S Nonfunctional Ribosomal RNA Decay and a Method for Detecting 8-oxoguanosine by RNA-Seq, Kelly A. Limoncelli

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Ensemble cryo-EM elucidates the mechanism of translation fidelity, Anna B. Loveland, Gabriel Demo, Nikolaus Grigorieff, and Andrei A. Korostelev

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Visualization of self-delivering hydrophobically modified siRNA cellular internalization, Socheata Ly, Deanna M. Navaroli, Marie-Claire Didiot, James Cardia, Lakshmipathi Pandarinathan, Julia F. Alterman, Kevin E. Fogarty, Clive Standley, Lawrence Lifshitz, Karl D. Bellve, Matthieu Prot, Dimas Echeverria, Silvia Corvera, and Anastasia Khvorova