Culture of whole mouse embryos at early postimplantation to organogenesis stages: developmental staging and methods
Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
2010-08-10
Metadata
Show full item recordAbstract
In vitro culture of whole mouse embryos enables the maintenance of growth and morphogenesis of postimplantation embryos outside the uterine environment. This technological advent facilitates the observation of the development of embryos in real time whereby cell lineage and tissue morphogenesis can be traced with appropriate vital cell labels and molecular markers. Embryos in culture are also amenable to direct experimental manipulations for elucidating developmental mechanisms of embryogenesis, germ layer formation, and embryonic patterning. This chapter outlines protocols for culturing mouse embryos at the immediate postimplantation period. We also present a system of developmental staging so that the outcome of different embryo culture studies may be assessed properly with reference to the precise developmental stage of the embryos used for the specific experiments.Source
Methods Enzymol. 2010;476:185-203. Link to article on publisher's siteDOI
10.1016/S0076-6879(10)76011-0Permanent Link to this Item
http://hdl.handle.net/20.500.14038/48773PubMed ID
20691867Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/S0076-6879(10)76011-0