Separation of functionally distinct subpopulations of Corynebacterium parvum-activated macrophages with predominantly stimulatory or suppressive effect on the cell-mediated cytotoxic T cell response
Department of Medicine, Division of Rheumatology
Animals; Ascitic Fluid; Cell Adhesion; Cell Separation; *Cytotoxicity, Immunologic; Female; Immunity, Cellular; *Immunosuppression; Leukemia, Experimental; Macrophages; Male; Mice; Mice, Inbred CBA; Propionibacterium acnes; Spleen; T-Lymphocytes; Time Factors
Immunology and Infectious Disease
Peritoneal cells (PEC) from mice injected ip with Corynebacterium parvum (CP) showed greatly enhanced suppressive activity on the growth of syngeneic tumor cells and on the generation of alloreactive cytotoxic T lymphocytes (CTL) in vitro. On the other hand, CP-activated PEC exhibited increased immunostimulatory (accessory or A cell) activity as measured by the restoration of the CTL response of nonadherent spleen cells. After fractionation of the CP-activated PEC according to cell size by velocity sedimentation, the mutually antagonistic A cell and immunosuppressive activities were clearly separated and found to be associated with functionally distinct subpopulations of macrophages. Thus A cell function was detected in fractions rich in small and medium sized macrophages which were probably derived from recently arrived monocytes. Immunosuppressive (and anti-tumor) activity was associated with the largest macrophages which were almost devoid of A cell function and probably represented a highly activated and differentiated macrophage subpopulation.
DOI of Published Version
Cell Immunol. 1979 Jan;42(1):28-41. doi:10.1016/0008-8749(79)90218-1 Link to article on publisher's website
Lee, Kwok-Choy; Kay, Jonathan; and Wong, Mabel, "Separation of functionally distinct subpopulations of Corynebacterium parvum-activated macrophages with predominantly stimulatory or suppressive effect on the cell-mediated cytotoxic T cell response" (1979). Rheumatology Publications and Presentations. 72.