Magnetic resonance contrast agents in optical clearing: Prospects for multimodal tissue imaging

UMMS Affiliation

Department of Radiology

Publication Date


Document Type



Analytical, Diagnostic and Therapeutic Techniques and Equipment | Bioimaging and Biomedical Optics | Biological and Chemical Physics | Radiology


Skin optical clearing effect ex vivo and in vivo was achieved by topical application of low molecular weight paramagnetic magnetic resonance contrast agents. This novel feature has not been explored before. By using collimated transmittance the diffusion coefficients of three clinically used magnetic resonance contrast agents, that is Gadovist, Magnevist and Dotarem as well as X-ray contrast agent Visipaque in mouse skin were determined ex vivo as (4.29 +/- 0.39) x 10(-7) cm(2) /s, (5.00 +/- 0.72) x 10(-7) cm(2) /s, (3.72 +/- 0.67) x 10(-7) cm(2) /s and (1.64 +/- 0.18) x 10(-7) cm(2) /s, respectively. The application of gadobutrol (Gadovist) resulted in efficient optical clearing that in general, was superior to other contrast agents tested and allowed to achieve: (a) more than 12-fold increase of transmittance over 10 minutes after application ex vivo; (b) markedly improved images of skin architecture obtained with optical coherence tomography; (c) an increase of the fluorescence intensity/background ratio in TagRFP-red fluorescent marker protein expressing tumor by five times after 15 minutes application into the skin in vivo. The obtained results have immediate implications for multimodality imaging because many contrast agents are capable of simultaneously enhancing the contrast of multiple imaging modalities.


optical clearing, MRI/CT contrast agents, mouse skin, diffusion coefficients, OCT, fluorescence imaging, multimodality

DOI of Published Version



Tuchina DK, Meerovich IG, Sindeeva OA, Zherdeva VV, Savitsky AP, Bogdanov AA Jr, Tuchin VV. Magnetic resonance contrast agents in optical clearing: Prospects for multimodal tissue imaging. J Biophotonics. 2020 Jul 20:e201960249. doi: 10.1002/jbio.201960249. Epub ahead of print. PMID: 32687263. Link to article on publisher's site

Journal/Book/Conference Title

Journal of biophotonics

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