Multimodality nuclear and fluorescence tumor imaging in mice using a streptavidin nanoparticle
Authors
Liang, Min MinLiu, Xinrong
Cheng, Dengfeng
Liu, Guozheng
Dou, Shuping
Wang, Yi
Rusckowski, Mary
Hnatowich, Donald J.
UMass Chan Affiliations
Department of RadiologyDocument Type
Journal ArticlePublication Date
2010-07-21Keywords
AnimalsCarbocyanines
Cell Line, Tumor
Cell Nucleus
Female
Fluorescence
Heterocyclic Compounds, 1-Ring
Humans
Mice
Mice, Nude
Nanoparticles
Neoplasms
Organometallic Compounds
Streptavidin
Xenograft Model Antitumor Assays
Medicinal-Pharmaceutical Chemistry
Nanomedicine
Neoplasms
Oncology
Radiology
Metadata
Show full item recordAbstract
Combining two or more different imaging modalities in the same agent can be of considerable value in molecular imaging. We describe the use of streptavidin nanoparticle-based complexes as multimodality imaging agents to achieve tumor detection in a mouse model by both fluorescence and nuclear imaging. Up to four biotinylated functionalities can be readily attached to these streptavidin nanoparticles without apparent influence on their properties and with reasonable pharmacokinetics and therefore may be ideally suited for multimodality imaging. By binding a biotinylated anti-Her2 Herceptin antibody to provide tumor targeting, a biotinylated DOTA chelator labeled with (111)ln and a biotinylated Cy5.5 fluorophore to a streptavidin nanoparticle, we demonstrated multimodality imaging in SUM190 (Her2+) tumor bearing mice on both an IVIS fluorescence camera and a NanoSPECT/CT small animal nuclear camera. The imaging results show high tumor accumulation and strong tumor-to-normal tissue contrast by both fluorescence and nuclear imaging. The subsequent biodistribution study confirmed the specific tumor accumulation in that tumor accumulation of radioactivity at 40 h was 21 ID%/g and therefore much higher than all other tissues including liver, heart, kidney, spleen, and muscle that accumulated 8.7, 2.5, 6.9, 7.2, and 1.9 ID%/g, respectively. In conclusion, the streptavidin nanoparticle under development in this laboratory was used effectively for multimodality imaging of tumor in mice by fluorescence and nuclear detection. Presumably, other imaging modalities could also be considered.Source
Bioconjug Chem. 2010 Jul 21;21(7):1385-8. doi: 10.1021/bc100081h. Link to article on publisher's siteDOI
10.1021/bc100081hPermanent Link to this Item
http://hdl.handle.net/20.500.14038/48411PubMed ID
20557066Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1021/bc100081h