Title

Sensors for Proteolytic Activity Visualization and Their Application in Animal Models of Human Diseases

UMMS Affiliation

Department of Radiology, Laboratory of Molecular Imaging Probes

Publication Date

2019-01-01

Document Type

Article

Disciplines

Analytical, Diagnostic and Therapeutic Techniques and Equipment | Biochemistry | Bioimaging and Biomedical Optics | Chemistry | Enzymes and Coenzymes | Molecular Biology | Radiology

Abstract

Various sensors designed for optical and photo(opto)acoustic imaging in living systems are becoming essential components of basic and applied biomedical research. Some of them including those developed for determining enzyme activity in vivo are becoming commercially available. These sensors can be used for various fluorescent signal detection methods: from whole body tomography to endoscopy with miniature cameras. Sensor molecules including enzyme-cleavable macromolecules carrying multiple quenched near-infrared fluorophores are able to deliver their payload in vivo and have long circulation time in bloodstream enabling detection of enzyme activity for extended periods of time at low doses of these sensors. In the future, more effective "activated" probes are expected to become available with optimized sensitivity to enzymatic activity, spectral characteristics suitable for intraoperative imaging of surgical field, biocompatibility and lack of immunogenicity and toxicity. New in vivo optical imaging methods such as the fluorescence lifetime and photo(opto)acoustic imaging will contribute to early diagnosis of human diseases. The use of sensors for in vivo optical imaging will include more extensive preclinical applications of experimental therapies. At the same time, the ongoing development and improvement of optical signal detectors as well as the availability of biologically inert and highly specific fluorescent probes will further contribute to the introduction of fluorescence imaging into the clinic.

Keywords

sensor molecules, optical and photo(opto)acoustic imaging, fluorescent signal detection

DOI of Published Version

10.1134/S0006297919140013

Source

Biochemistry (Mosc). 2019 Jan;84(Suppl 1):S1-S18. doi: 10.1134/S0006297919140013. Link to article on publisher's site

Journal/Book/Conference Title

Biochemistry. Biokhimiia

Related Resources

Link to Article in PubMed

PubMed ID

31213192

Share

COinS