Division of Cell Biology and Imaging, Department of Radiology; Witman Lab
Amino Acids, Peptides, and Proteins | Cell Biology | Cells | Cellular and Molecular Physiology | Diagnosis | Genetic Phenomena
Nearly all motile cilia have a "9+2" axoneme containing a central apparatus (CA), consisting of two central microtubules with projections, that is essential for motility. To date, only 22 proteins are known to be CA components. To identify new candidate CA proteins, we used mass spectrometry to compare axonemes of wild-type Chlamydomonas and a CA-less mutant. We identified 44 novel candidate CA proteins, of which 13 are conserved in humans. Five of the latter were studied more closely, and all five localized to the CA; therefore, most of the other candidates are likely to also be CA components. Our results reveal that the CA is far more compositionally complex than previously recognized and provide a greatly expanded knowledge base for studies to understand the architecture of the CA and how it functions. The discovery of the new conserved CA proteins will facilitate genetic screening to identify patients with a form of primary ciliary dyskinesia that has been difficult to diagnose.
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© 2019 Zhao et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
DOI of Published Version
J Cell Biol. 2019 Jun 3;218(6):2051-2070. doi: 10.1083/jcb.201902017. Epub 2019 May 15. Link to article on publisher's site
The Journal of cell biology
Zhao, Lei; Hou, Yuqing; Picariello, Tyler; Craige, Branch; and Witman, George B., "Proteome of the central apparatus of a ciliary axoneme" (2019). Radiology Publications and Presentations. 471.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.