Platelet decoys inhibit thrombosis and prevent metastatic tumor formation in preclinical models
New England Center for Stroke Research, Department of Radiology
Cardiovascular Diseases | Hemic and Immune Systems | Neoplasms | Nervous System Diseases | Radiology
Platelets are crucial for normal hemostasis; however, their hyperactivation also contributes to many potentially lethal pathologies including myocardial infarction, stroke, and cancer. We hypothesized that modified platelets lacking their aggregation and activation capacity could act as reversible inhibitors of platelet activation cascades. Here, we describe the development of detergent-extracted human modified platelets (platelet decoys) that retained platelet binding functions but were incapable of functional activation and aggregation. Platelet decoys inhibited aggregation and adhesion of platelets on thrombogenic surfaces in vitro, which could be immediately reversed by the addition of normal platelets; in vivo in a rabbit model, pretreatment with platelet decoys inhibited arterial injury-induced thromboembolism. Decoys also interfered with platelet-mediated human breast cancer cell aggregation, and their presence decreased cancer cell arrest and extravasation in a microfluidic human microvasculature on a chip. In a mouse model of metastasis, simultaneous injection of the platelet decoys with tumor cells inhibited metastatic tumor growth. Thus, our results suggest that platelet decoys might represent an effective strategy for obtaining antithrombotic and antimetastatic effects.
DOI of Published Version
Sci Transl Med. 2019 Feb 13;11(479). pii: eaau5898. doi: 10.1126/scitranslmed.aau5898. Link to article on publisher's site
Science translational medicine
Papa, Anne-Laure; Jiang, Amanda; Korin, Netanel; Chen, Michelle B.; Langan, Erin T.; Waterhouse, Anna; Nash, Emma; Caroff, Jildaz; Graveline, Amanda; Vernet, Andyna; Mammoto, Akiko; Mammoto, Tadanori; Jain, Abhishek; Kamm, Roger D.; Gounis, Matthew J.; and Ingber, Donald E., "Platelet decoys inhibit thrombosis and prevent metastatic tumor formation in preclinical models" (2019). Radiology Publications and Presentations. 456.