UMMS Affiliation
Department of Radiology, Division of Cell Biology and Imaging
Publication Date
2017-09-01
Document Type
Article
Disciplines
Cell Biology | Cellular and Molecular Physiology | Molecular Biology
Abstract
Cilia are assembled via intraflagellar transport (IFT). The IFT machinery is composed of motors and multi-subunit particles, termed IFT-A and IFT-B, that carry cargo into the cilium. Knowledge of how the IFT subunits interact with their cargo is of critical importance for understanding how the unique ciliary domain is established. We previously reported a Chlamydomonas mutant, ift46-1, that fails to express the IFT-B protein IFT46, has greatly reduced levels of other IFT-B proteins, and assembles only very short flagella. A spontaneous suppression of ift46-1 restored IFT-B levels and enabled growth of longer flagella, but the flagella lacked outer dynein arms. Here, we show that the suppression is due to insertion of the transposon MRC1 into the ift46-1 allele, causing the expression of a fusion protein including the IFT46 C-terminal 240 amino acids. The IFT46 C-terminus can assemble into and stabilize IFT-B, but does not support transport of outer arm dynein into flagella. ODA16, a cargo adaptor specific for outer arm dynein, also fails to be imported into the flagella in the absence of the IFT46 N-terminus. We conclude that IFT46, ODA16, and the outer dynein arm interact for IFT of the latter.
Keywords
cilia, flagella, intraflagellar transport, IFT
Rights and Permissions
© 2017 Hou and Witman. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). Publisher PDF posted as allowed by the publisher's author rights policy at http://www.molbiolcell.org/site/misc/ifora.xhtml.
DOI of Published Version
10.1091/mbc.E17-03-0172
Source
Mol Biol Cell. 2017 Sep 1;28(18):2420-2433. doi: 10.1091/mbc.E17-03-0172. Epub 2017 Jul 12. Link to article on publisher's site
Journal/Book/Conference Title
Molecular biology of the cell
Related Resources
PubMed ID
28701346
Repository Citation
Hou, Yuqing and Witman, George B., "The N-terminus of IFT46 mediates intraflagellar transport of outer arm dynein and its cargo-adaptor ODA16" (2017). Radiology Publications and Presentations. 345.
https://escholarship.umassmed.edu/radiology_pubs/345
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.
Included in
Cell Biology Commons, Cellular and Molecular Physiology Commons, Molecular Biology Commons