UMMS Affiliation
Department of Cell and Developmental Biology; Department of Radiology; Witman Lab
Publication Date
2017-05-31
Document Type
Article
Disciplines
Cell Biology | Cellular and Molecular Physiology | Developmental Biology
Abstract
Intraflagellar transport (IFT) trains, multimegadalton assemblies of IFT proteins and motors, traffic proteins in cilia. To study how trains assemble, we employed fluorescence protein-tagged IFT proteins in Chlamydomonas reinhardtii. IFT-A and motor proteins are recruited from the cell body to the basal body pool, assembled into trains, move through the cilium, and disperse back into the cell body. In contrast to this 'open' system, IFT-B proteins from retrograde trains reenter the pool and a portion is reused directly in anterograde trains indicating a 'semi-open' system. Similar IFT systems were also observed in Tetrahymena thermophila and IMCD3 cells. FRAP analysis indicated that IFT proteins and motors of a given train are sequentially recruited to the basal bodies. IFT dynein and tubulin cargoes are loaded briefly before the trains depart. We conclude that the pool contains IFT trains in multiple stages of assembly queuing for successive release into the cilium upon completion.
Keywords
IFT, cell biology, chlamydomonas reinhardtii, flagella
Rights and Permissions
© 2017, Wingfield et al.
DOI of Published Version
10.7554/eLife.26609
Source
Elife. 2017 May 31;6. pii: e26609. doi: 10.7554/eLife.26609. Link to article on publisher's site
Journal/Book/Conference Title
eLife
Related Resources
PubMed ID
28562242
Repository Citation
Brown, Jason M.; Picariello, Tyler; Cochran, Deborah A.; Witman, George B.; and Lechtreck, Karl, "IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium" (2017). Radiology Publications and Presentations. 315.
https://escholarship.umassmed.edu/radiology_pubs/315
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Cell Biology Commons, Cellular and Molecular Physiology Commons, Developmental Biology Commons
Comments
Full author list omitted for brevity. For the full list of authors, see article.