Comparison of 18F PET and 99mTc SPECT imaging in phantoms and in tumored mice

UMMS Affiliation

Department of Radiology, Division of Nuclear Medicine

Publication Date


Document Type



Animals; Fluorine Radioisotopes; Mice; Morpholines; Morpholinos; Nanoparticles; Neoplasm Transplantation; Neoplasms, Experimental; Organotechnetium Compounds; Phantoms, Imaging; Positron-Emission Tomography; Streptavidin; Tomography, Emission-Computed, Single-Photon


Medicinal-Pharmaceutical Chemistry | Nanomedicine | Radiochemistry | Radiology | Therapeutics


Our objective was to compare the performance of a micro-single photon emission computed tomography (micro-SPECT) with that of a micro-positron emission tomography (microPET) in a Her2+ tumored mice using an anti-Her2 nanoparticle radiolabeled with (99m)Tc and (18)F. Camera performance was first compared using phantoms; then a tumored mouse administered the (99m)Tc-nanoparticle was imaged on a Bioscan NanoSPECT/CT, while another tumored mouse received the identical nanoparticle, labeled now with (18)F, and was imaged on a Philips Mosaic HP PET camera. The nanoparticle was radiolabeled with (99m)Tc via MAG(3) chelation and with (18)F via SFB as an intermediate. Phantom imaging showed that the resolution of the SPECT camera was clearly superior, but even with 4 heads and multipinhole collimators, detection sensitivity was 15-fold lower. Radiolabeling of the nanoparticle by chelation with (99m)Tc was considerably easier and safer than manual covalent attachment of (18)F. Both cameras provided accurate quantitation of radioactivity over a broad range. In conclusion, when deciding between (99m)Tc vs (18)F, an advantage rests with the chelation of (99m)Tc over covalent attachment of (18)F, achieved manually or otherwise, but with these small animal cameras, this choice also results in trading lower sensitivity for higher resolution.


Positron Emission Tomography (PET), Single Photon Emission Computed Tomography (SPECT), Computed Tomography (CT), F-18, Molecular Imaging

DOI of Published Version



Bioconjug Chem. 2010 Aug 18;21(8):1565-70. doi: 10.1021/bc1001467. Link to article on publisher's site

Journal/Book/Conference Title

Bioconjugate chemistry

Related Resources

Link to Article in PubMed

PubMed ID