Gamma interferon responses to Plasmodium falciparum liver-stage antigen 1 and thrombospondin-related adhesive protein and their relationship to age, transmission intensity, and protection against malaria
Department of Quantitative Health Sciences; Department of Pediatrics
Adolescent; Adult; Aging; Animals; Antigens, Protozoan; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Humans; Interferon-gamma; Kenya; Malaria, Falciparum; Plasmodium falciparum; Protozoan Proteins
Biostatistics | Epidemiology | Health Services Research | Immunology and Infectious Disease | Pediatrics
Gamma interferon (IFN-gamma) responses to the Plasmodium falciparum antigens liver-stage antigen 1 (LSA-1) and thrombospondin-related adhesive protein (TRAP) are thought to be important in protection against malaria. Optimal methods of testing and the effects of age and transmission intensity on these responses are unknown. IFN-gamma responses to LSA-1 and TRAP peptides were assessed by the enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA) in children and adults from areas of stable and unstable malaria transmission in Kenya. Adults in the areas of stable and unstable transmission had similar frequencies and levels of IFN-gamma responses to LSA-1 and TRAP as determined by ELISPOT and ELISA. In contrast, IFN-gamma responses to the LSA-1 T3 peptide (assessed by ELISPOT) and to any LSA-1 peptide (assessed by ELISA) were less frequent in children in the area of unstable transmission than in children in the area of stable transmission. IFN-gamma responses to LSA-1 were more frequently detected by ELISA than by ELISPOT in the stable-transmission area. IFN-gamma responses detected by ELISA and ELISPOT did not correlate with each other. In children in the stable-transmission area, IFN-gamma responses to LSA-1 peptides assessed by ELISA, but not by ELISPOT, were associated with protection against clinical malaria and anemia. IFN-gamma responses to LSA-1 appear to require repeated P. falciparum exposure and/or increased age and, as measured by ELISA, are associated with protection against clinical malaria and anemia.
DOI of Published Version
Infect Immun. 2004 Sep;72(9):5135-42. Link to article on publisher's site
Infection and immunity
John, Chandy C.; Moormann, Ann M.; Sumba, Peter Odada; Ofulla, Ayub V.; Pregibon, Daniel C.; and Kazura, James W., "Gamma interferon responses to Plasmodium falciparum liver-stage antigen 1 and thrombospondin-related adhesive protein and their relationship to age, transmission intensity, and protection against malaria" (2004). Quantitative Health Sciences Publications and Presentations. 389.