Department of Biochemistry and Molecular Pharmacology
Amino Acids, Peptides, and Proteins | Biochemistry | Enzymes and Coenzymes | Molecular and Cellular Neuroscience | Nervous System Diseases
Amyotrophic lateral sclerosis (ALS) is a deadly neurodegenerative disease characterized by loss of motor neurons, paralysis and eventual death. The mechanism of ALS is still incompletely understood, and the disease is to date without an effective remedy. Protein arginine deiminase 2 (PAD2) converts peptidyl-Arginine into peptidyl-Citrulline, a post-translational modification referred to as citrullination. Aberrant expression of PAD2 and protein citrullination are increased in several neurodegenerative conditions. Whether this increase is involved in ALS is unknown. In this study, we investigated PAD2 and protein citrullination in two genetic mouse models of ALS expressing human mutant SOD1G93A and PFN1C71G, respectively, and in post-mortem human ALS spinal cord. We show that the expression of PAD2 mRNA and protein expressions are increased progressively along with the ALS progression. Additionally, protein citrullination is increase following the same trend. These changes occur in areas with the most severe motor neuron degeneration including the spinal cord, and brainstem. We show that the increase in PAD2 and citrullinated proteins occur specifically in astrocytes coinciding with the development of reactive astrogliosis. Finally, we show that citrullinated proteins form non-astrocyte aggregate patterns; and are dominantly expressed in insoluble protein fractions. These results demonstrate that PAD2 dysregulation and increased protein citrullination are key characteristics of reactive astrogliosis, and possibly drive some type of protein aggregation in the pathogenesis of ALS. Because protein citrullination alters protein functions, our results suggest that PAD2 and protein citrullination play a role in astrogliosis and astrocytic toxicity in ALS and other neurodegenerative conditions.
Amyotrophic lateral sclerosis, ALS, Protein arginine deiminase 2, PAD2, protein citrullination, astrogliosis, astrocytic toxicity
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Copyright © 2020 The Author(s). This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
25th Annual University of Massachusetts Medical School Research Retreat 2020
Yusuf I, Qiao T, Tilvawala R, Thompson PR, Xu Z. (2020). PAD2 Dysregulation and Abnormal Protein Citrullination in ALS Disease Models. University of Massachusetts Medical School Publications. https://doi.org/10.13028/msqa-5268. Retrieved from https://escholarship.umassmed.edu/publications/43
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