Title
DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity.
UMMS Affiliation
Thompson Lab; Department of Biochemistry and Molecular Pharmacology
Publication Date
2017-03-18
Document Type
Book Chapter
Disciplines
Biochemistry | Enzymes and Coenzymes | Medicinal-Pharmaceutical Chemistry | Molecular Biology
Abstract
We here describe a quick and easy method to quantitatively measure in vitro acetylation activity of not only N-terminal acetyltransferase (NAT) enzymes, but acetyltransferases using acetyl-coenzyme A as an acetyl donor in general.
Keywords
Acetyl Coenzyme A, Acetylation, Dithionitrobenzoic Acid, Enzyme Activation, Enzyme Assays, In Vitro Techniques, N-Terminal Acetyltransferases, Substrate Specificity
DOI of Published Version
10.1007/978-1-4939-6850-3_2
Source
Methods Mol Biol. 2017;1574:9-15. doi: 10.1007/978-1-4939-6850-3_2.
Journal/Book/Conference Title
Methods in molecular biology (Clifton, N.J.)
PubMed ID
28315240
Repository Citation
Foyn, Havard; Thompson, Paul R.; and Arnesen, Thomas, "DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity." (2017). University of Massachusetts Medical School Publications. 3.
https://escholarship.umassmed.edu/publications/3