Title

DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity.

UMMS Affiliation

Thompson Lab; Department of Biochemistry and Molecular Pharmacology

Publication Date

2017-03-18

Document Type

Book Chapter

Disciplines

Biochemistry | Enzymes and Coenzymes | Medicinal-Pharmaceutical Chemistry | Molecular Biology

Abstract

We here describe a quick and easy method to quantitatively measure in vitro acetylation activity of not only N-terminal acetyltransferase (NAT) enzymes, but acetyltransferases using acetyl-coenzyme A as an acetyl donor in general.

Keywords

Acetyl Coenzyme A, Acetylation, Dithionitrobenzoic Acid, Enzyme Activation, Enzyme Assays, In Vitro Techniques, N-Terminal Acetyltransferases, Substrate Specificity

DOI of Published Version

10.1007/978-1-4939-6850-3_2

Source

Methods Mol Biol. 2017;1574:9-15. doi: 10.1007/978-1-4939-6850-3_2.

Journal/Book/Conference Title

Methods in molecular biology (Clifton, N.J.)

PubMed ID

28315240

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