The Program in Molecular Medicine at the University of Massachusetts Medical School was established in a new research building in 1989. Faculty include both clinical investigators and basic scientists, many of whom focus their research on human disease processes. The laboratory groups and core facilities that compose Molecular Medicine represent many disciplines in the bio-medical sciences: biochemistry and molecular biology, cell biology, medicine, immunology, molecular genetics and microbiology, pediatrics, pharmacology, physiology, genomics, bioinformatics and proteomics. This diverse scientific environment makes the Program an exceptional training ground for graduate students and postdoctoral fellows, who in turn contribute much to its vitality and excitement. This collection showcases publications and presentations written by faculty and researchers of the Program in Molecular Medicine.
Publications from 1994
Non-syncytium-inducing HIV type 1 isolated from infected individuals replicates in MT-2 cells, Serene Elizabeth Forte, Kevin S. Byron, John L. Sullivan, and Mohan Somasundaran
The RNA polymerase I transcription factor, upstream binding factor, interacts directly with the TATA box-binding protein, Hyockman Kwon and Michael R. Green
Synergistic activation of simian immunodeficiency virus and human immunodeficiency virus type 1 transcription by retinoic acid and phorbol ester through an NF-kappa B-independent mechanism, Joseph Walter Maciaszek, David A. Talmage, and Gregory A. Viglianti
Human transcription factor YY1 represses human immunodeficiency virus type 1 transcription and virion production, David M. Margolis, Mohan Somasundaran, and Michael R. Green
Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement, Craig L. Peterson, Andrew K. Dingwall, and Matthew P. Scott
Yeast TAFIIS in a multisubunit complex required for activated transcription, Joseph C. Reese, Lynne Marie Apone, Scott S. Walker, Loree A. Griffin, and Michael R. Green
Differential intracellular localizations of GDP dissociation inhibitor isoforms. Insulin-dependent redistribution of GDP dissociation inhibitor-2 in 3T3-L1 adipocytes, Assia Shisheva, Joanne M. Buxton, and Michael P. Czech
Cloning, characterization, and expression of a novel GDP dissociation inhibitor isoform from skeletal muscle, Assia Shisheva, Thomas C. Sudhof, and Michael P. Czech
Signal transduction by tumor necrosis factor mediated by JNK protein kinases, Hayla Karen Sluss, Tamera Barrett, Benoit Derijard, and Roger J. Davis
Localization of HIV RNA in mitochondria of infected cells: potential role in cytopathogenicity, Mohan Somasundaran, Maria L. Zapp, Lars K. Beattie, Lizhen Pang, Kevin S. Byron, Gary J. Bassell, John L. Sullivan, and Robert H. Singer
The native structure of the activated Raf protein kinase is a membrane-bound multi-subunit complex, Markus Wartmann and Roger J. Davis
Publications from 1993
Redistribution of clathrin-coated vesicle adaptor complexes during adipocytic differentiation of 3T3-L1 cells, Ranjan Chakrabarti, Marguerite Joly, and Silvia Corvera
Insulin action on the internalization of the GLUT4 glucose transporter in isolated rat adipocytes, Michael P. Czech and Joanne M. Buxton
Exofacial epitope-tagged glucose transporter chimeras reveal COOH-terminal sequences governing cellular localization, Michael P. Czech, Anil Chawla, Chee-Wai Woon, Joanne M. Buxton, Michal Armoni, Wei Tang, Marguerite Joly, and Silvia Corvera
The mitogen-activated protein kinase signal transduction pathway, Roger J. Davis
Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus, Fernando A. Gonzalez, Alpna Seth, David L. Raden, Douglas S. Bowman, Fredric S. Fay, and Roger J. Davis
Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62, Shashi Gupta, Alpna Seth, and Roger J. Davis
The ras signaling pathway mimics insulin action on glucose transporter translocation, Lynn M. Kozma, Kurt Baltensperger, Jes K. Klarlund, A Porras, E Santos, and Michael P. Czech
E1A functions as a coactivator of retinoic acid-dependent retinoic acid receptor-beta 2 promoter activation, Frank A. E. Kruyt, Gert E. Folkers, Albertha J. M. Walhout, Bas-jan M. van der Leede, and Paul T. van der Saag
The cAMP response element binding protein, CREB, is a potent inhibitor of diverse transcriptional activators, Frederic P. Lemaigre, Christopher I. Ace, and Michael R. Green
cPLA2 is phosphorylated and activated by MAP kinase, Lih-Ling Lin, Markus Wartmann, Alice Y. Lin, John L. Knop, Alpna Seth, and Roger J. Davis
sieB and esc genes of Bacteriophage P22: A Dissertation, Koustubh Ranade
Mitogen-activated protein kinase stimulation by a tyrosine kinase-negative epidermal growth factor receptor, Erica Marie Selva, David L. Raden, and Roger J. Davis
Cell cycle regulation of the c-Myc transcriptional activation domain, Alpna Seth, Shashi Gupta, and Roger J. Davis
Assignment of the nuclear mitotic apparatus protein NuMA gene to human chromosome 11q13, Cynthia A. Sparks, Peter Lawrence Bangs, Gerard P. McNeil, Jeanne B. Lawrence, and Edward G. Fey
Induction of renal adenocarcinoma by a nonmutated erbB oncogene, Cherie A. Taglienti, Barbara F. Banner, Roger J. Davis, and Harriet L. Robinson
Publications from 1992
Catalysis of serine and tyrosine autophosphorylation by the human insulin receptor, Kurt Baltensperger, Robert E. Lewis, Chee-Wai Woon, Prabhakar Vissavajjhala, Alonzo H. Ross, and Michael P. Czech
Activation of cell surface glucose transporters measured by photoaffinity labeling of insulin-sensitive 3T3-L1 adipocytes, Scott A. Harrison, Brian M. Clancy, Anna Pessino, and Michael P. Czech
Glucose transporter oligomeric structure determines transporter function. Reversible redox-dependent interconversions of tetrameric and dimeric GLUT1, Daniel N. Hebert and Anthony Carruthers
Protein tyrosine kinase activities of the epidermal growth factor receptor and ErbB proteins: correlation of oncogenic activation with altered kinetics, Nandini Nair, Roger J. Davis, and Harriet L. Robinson
Signal transduction within the nucleus by mitogen-activated protein kinase, Alpna Seth, Fernando A. Gonzalez, Shashi Gupta, David L. Raden, and Roger J. Davis
Rapid screening of cloned DNA fragments for specific mutations, Steven J. Theroux and Roger J. Davis
Mutational removal of the major site of serine phosphorylation of the epidermal growth factor receptor causes potentiation of signal transduction: role of receptor down-regulation, Steven J. Theroux, Krista Stanley, Debra A. Campbell, and Roger J. Davis
Increased oncogenic potential of ErbB is associated with the loss of a COOH-terminal domain serine phosphorylation site, Steven J. Theroux, Cherie A. Taglienti, Nandini Nair, Janice L. Countaway, Harriet L. Robinson, and Roger J. Davis
Residues in the longitudinal, hydrophobic strip-of-helix relate to terminations and crossings of alpha-helices, Stephen R. Vazquez, Dennis Z. Kuo, Christopher M. Bositis, Larry W. Hardy, Robert A. Lew, and Robert E. Humphreys
Role of TAR RNA splicing in translational regulation of simian immunodeficiency virus from rhesus macaques, Gregory A. Viglianti, Eric P. Rubinstein, and Karen Lorraine Graves
Purification and characterization of a cytosolic insulin-stimulated serine kinase from rat liver, Lynn Wolfe, Andrew P. Bradford, Jes K. Klarlund, and Michael P. Czech
Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit, Meng Zhang, Phillip D. Zamore, Maria Carmo-Fonseca, Angus I. Lamond, and Michael R. Green
Publications from 1991
Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c-myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase, Elvira Alvarez, Ingrid C. Northwood, Fernando A. Gonzalez, Debra A. Latour, Alpna Seth, Cory Abate, Tom Curran, and Roger J. Davis
Constitutive phosphorylation of the epidermal growth factor receptor blocks mitogenic signal transduction, Sharon Bowen, Krista Stanley, Erica Marie Selva, and Roger J. Davis
Extracellular release as the major degradative pathway of the insulin-like growth factor II/mannose 6-phosphate receptor, Kevin B. Clairmont and Michael P. Czech
Protein synthesis inhibitors activate glucose transport without increasing plasma membrane glucose transporters in 3T3-L1 adipocytes, Brian M. Clancy, Scott A. Harrison, Joanne M. Buxton, and Michael P. Czech
Acute inhibition of insulin-stimulated glucose transport by the phosphatase inhibitor, okadaic acid, Silvia Corvera, Steven R. Jaspers, and Marguerite Pasceri
The nuclear matrix: defining structural and functional roles, Edward G. Fey, Peter Lawrence Bangs, Cynthia A. Sparks, and Paul R. Odgren
Mutational analysis of the cytoplasmic tail of the human transferrin receptor. Identification of a sub-domain that is required for rapid endocytosis, Nuria Girones, Elvira Alvarez, Alpna Seth, I-Mei Lin, Debra A. Latour, and Roger J. Davis
Identification of substrate recognition determinants for human ERK1 and ERK2 protein kinases, Fernando A. Gonzalez, David L. Raden, and Roger J. Davis
Evidence that erythroid-type glucose transporter intrinsic activity is modulated by cadmium treatment of mouse 3T3-L1 cells, Scott A. Harrison, Joanne M. Buxton, Brian M. Clancy, and Michael P. Czech
Suppressed intrinsic catalytic activity of GLUT1 glucose transporters in insulin-sensitive 3T3-L1 adipocytes, Scott A. Harrison, Joanne M. Buxton, and Michael P. Czech
Enhanced casein kinase II activity in COS-1 cells upon overexpression of either its catalytic or noncatalytic subunit, Robin A. Heller-Harrison and Michael P. Czech
An insulin-stimulated kemptide kinase purified from rat liver is deactivated by phosphatase 2A, Jes K. Klarlund, Steven R. Jaspers, Nazer Khalaf, Andrew P. Bradford, Thomas B. Miller Jr., and Michael P. Czech
Isolation and characterization of two growth factor-stimulated protein kinases that phosphorylate the epidermal growth factor receptor at threonine 669, Ingrid C. Northwood, Fernando A. Gonzalez, Markus Wartmann, David L. Raden, and Roger J. Davis
Evidence that functional erythrocyte-type glucose transporters are oligomers, Anna Pessino, Daniel N. Hebert, Chee-Wai Woon, Scott A. Harrison, Brian M. Clancy, Joanne M. Buxton, Anthony Carruthers, and Michael P. Czech
A phosphorylation site located in the NH2-terminal domain of c-Myc increases transactivation of gene expression, Alpna Seth, Elvira Alvarez, Shashi Gupta, and Roger J. Davis
Highly restricted distributions of hydrophobic and charged amino acids in longitudinal quadrants of alpha-helices, Rochelle R. Torgerson, Robert A. Lew, Victor E. Reyes, Larry W. Hardy, and Robert E. Humphreys
Oligomerization and RNA binding domains of the type 1 human immunodeficiency virus Rev protein: a dual function for an arginine-rich binding motif, Maria L. Zapp, Thomas J. Hope, Tristram G. Parslow, and Michael R. Green
Publications from 1990
Inhibition of the receptor-mediated endocytosis of diferric transferrin is associated with the covalent modification of the transferrin receptor with palmitic acid, Elvira Alvarez, Nuria Girones, and Roger J. Davis
The Structure, Function, and Regulation of Insulin-like Growth factor II/Mannose 6-phosphate Receptor Forms: a Thesis, Kevin B. Clairmont
Insulin injection increases the levels of serum receptors for transferrin and insulin-like growth factor-II/mannose-6-phosphate in intact rats, Kevin B. Clairmont and Michael P. Czech
Hexose transport stimulation and membrane redistribution of glucose transporter isoforms in response to cholera toxin, dibutyryl cyclic AMP, and insulin in 3T3-L1 adipocytes, Brian M. Clancy and Michael P. Czech
Insulin stimulates the assembly of cytosolic clathrin onto adipocyte plasma membranes, Silvia Corvera
Enhanced phosphorylation of a coated vesicle polypeptide in response to insulin stimulation of rat adipocytes, Silvia Corvera and Renold J. Capocasale
Multisite phosphorylation of the epidermal growth factor receptor. Use of site-directed mutagenesis to examine the role of serine/threonine phosphorylation, Janice L. Countaway, Patricia A. McQuilkin, Nuria Girones, and Roger J. Davis
Insulin regulation of hexose transport in mouse 3T3-L1 cells expressing the human HepG2 glucose transporter, Scott A. Harrison, Joanne M. Buxton, Brian M. Clancy, and Michael P. Czech
Insulin action on activity and cell surface disposition of human HepG2 glucose transporters expressed in Chinese hamster ovary cells, Scott A. Harrison, Joanne M. Buxton, Amy L. Helgerson, Richard G. MacDonald, Francis J. Chlapowski, Anthony Carruthers, and Michael P. Czech
Purification of a novel insulin-stimulated protein kinase from rat liver, Jes K. Klarlund, Andrew P. Bradford, Maria G. Milla, and Michael P. Czech
Insulin-sensitive phosphorylation of serine 1293/1294 on the human insulin receptor by a tightly associated serine kinase, Robert E. Lewis, G. Perry Wu, Richard G. MacDonald, and Michael P. Czech
Translational control by cytoplasmic polyadenylation during Xenopus oocyte maturation: characterization of cis and trans elements and regulation by cyclin/MPF, Laura Lynn McGrew and Joel D. Richter
Signal transduction by the epidermal growth factor receptor after functional desensitization of the receptor tyrosine protein kinase activity, Ingrid C. Northwood and Roger J. Davis
Maternal mRNA expression in early development: regulation at the 3' end, Joel D. Richter, Jeannie Paris, and Laura Lynn McGrew
Publications from 1989
Intermolecular disulfide bonds are not required for the expression of the dimeric state and functional activity of the transferrin receptor, Elvira Alvarez, Nuria Girones, and Roger J. Davis
Chicken and Xenopus mannose 6-phosphate receptors fail to bind insulin-like growth factor II, Kevin B. Clairmont and Michael P. Czech
Reconstitution of epidermal growth factor receptor transmodulation by platelet-derived growth factor in Chinese hamster ovary cells, Janice L. Countaway, Nuria Girones, and Roger J. Davis
Mechanism of phosphorylation of the epidermal growth factor receptor at threonine 669, Janice L. Countaway, Ingrid C. Northwood, and Roger J. Davis
Serum form of the rat insulin-like growth factor II/mannose 6-phosphate receptor is truncated in the carboxyl-terminal domain, Richard G. MacDonald, Mark A. Tepper, Kevin B. Clairmont, Susan B. Perregaux, and Michael P. Czech
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element, Laura Lynn McGrew, Eva Dworkin-Rastl, Mark B. Dworkin, and Joel D. Richter
Protein kinase C inhibition of the epidermal growth factor receptor tyrosine protein kinase activity is independent of the oligomeric state of the receptor, Ingrid C. Northwood and Roger J. Davis
Publications from 1988
A highly phosphorylated subpopulation of insulin-like growth factor II/mannose 6-phosphate receptors is concentrated in a clathrin-enriched plasma membrane fraction, Silvia Corvera, Kimberly L. Folander, Kevin B. Clairmont, and Michael P. Czech
Tyrosine phosphorylation of the receptor for insulin-like growth factor II is inhibited in plasma membranes from insulin-treated rat adipocytes, Silvia Corvera, Keith A. Yagaloff, Robert E. Whitehead, and Michael P. Czech
Insulin receptor signaling. Activation of multiple serine kinases, Michael P. Czech, Jes K. Klarlund, Keith A. Yagaloff, Andrew P. Bradford, and Robert E. Lewis
Insulin-like growth factor I and insulin rapidly increase casein kinase II activity in BALB/c 3T3 fibroblasts, Jes K. Klarlund and Michael P. Czech
Activation of the epidermal growth factor receptor tyrosine protein kinase in the absence of receptor oligomerization, Ingrid C. Northwood and Roger J. Davis
Publications from 1987
Insulin-like growth factor I and epidermal growth factor regulate the expression of transferrin receptors at the cell surface by distinct mechanisms, Roger J. Davis, Mark Faucher, Lori Kuck Racaniello, Anthony Carruthers, and Michael P. Czech