The Program in Molecular Medicine at the University of Massachusetts Medical School was established in a new research building in 1989. Faculty include both clinical investigators and basic scientists, many of whom focus their research on human disease processes. The laboratory groups and core facilities that compose Molecular Medicine represent many disciplines in the bio-medical sciences: biochemistry and molecular biology, cell biology, medicine, immunology, molecular genetics and microbiology, pediatrics, pharmacology, physiology, genomics, bioinformatics and proteomics. This diverse scientific environment makes the Program an exceptional training ground for graduate students and postdoctoral fellows, who in turn contribute much to its vitality and excitement. This collection showcases publications and presentations written by faculty and researchers of the Program in Molecular Medicine.

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Publications from 1996

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Intramolecular inhibition of activating transcription factor-2 function by its DNA-binding domain, Xiao-Yong Li and Michael R. Green

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Ex vivo expansion of murine marrow cells with interleukin-3 (IL-3), IL-6, IL-11, and stem cell factor leads to impaired engraftment in irradiated hosts, Stefan O. Peters, Ellen L. W. Kittler, Hayley S. Ramshaw, and Peter J. Quesenberry

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MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway, Joel Raingeaud, Alan J. Whitmarsh, Tamera Barrett, Benoit Derijard, and Roger J. Davis

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Functional analysis of the DNA-stimulated ATPase domain of yeast SWI2/SNF2, Emilie Richmond and Craig L. Peterson

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The rat T-cell surface protein RT6 is associated with src family tyrosine kinases and generates an activation signal, Mark R. Rigby, Rita Bortell, Dale L. Greiner, Michael P. Czech, Jes K. Klarlund, John P. Mordes, and Aldo A. Rossini

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Ordered water molecules as key allosteric mediators in a cooperative dimeric hemoglobin, William E. Royer, Animesh Dev Pardanani, Quentin H. Gibson, Eric S. Peterson, and Joel M. Friedman

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Potential sites of PI-3 kinase function in the endocytic pathway revealed by the PI-3 kinase inhibitor, wortmannin, Howard S. Shpetner, Marguerite Joly, David Alan Hartley, and Silvia Corvera

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A JNK signal transduction pathway that mediates morphogenesis and an immune response in Drosophila, Hayla Karen Sluss, Zhiqiang Han, Tamera Barrett, Deborah C. I. Goberdhan, Clive Wilson, Roger J. Davis, and Y. Tony Ip

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Mouse p170 is a novel phosphatidylinositol 3-kinase containing a C2 domain, Joseph V. Virbasius, Adilson L. Guilherme, and Michael P. Czech

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Transcription activation in cells lacking TAFIIS, Scott S. Walker, Joseph C. Reese, Lynne Marie Apone, and Michael R. Green

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Localization of HIV-1 RNA in mammalian nuclei, Guohong Zhang, Maria L. Zapp, Guochen Yan, and Michael R. Green

Publications from 1995

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Disassembly of Son-of-sevenless proteins from Grb2 during p21ras desensitization by insulin, Andrew D. Cherniack, Jes K. Klarlund, Bruce R. Conway, and Michael P. Czech

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Sequential recognition of the pre-mRNA branch point by U2AF65 and a novel spliceosome-associated 28-kDa protein, Rajesh K. Gaur, Juan Valcarcel, and Michael R. Green

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Specific association of the beta isoform of the p85 subunit of phosphatidylinositol-3 kinase with the proto-oncogene c-cbl, David Alan Hartley, Herman Meisner, and Silvia Corvera

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Insulin regulation of membrane-associated insulin receptor substrate 1, Robin A. Heller-Harrison, Michelle Morin, and Michael P. Czech

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Phosphatidylinositol 3-kinase activity is required at a postendocytic step in platelet-derived growth factor receptor trafficking, Marguerite Joly, Andrius Kazlauskas, and Silvia Corvera

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In vivo functional analysis of the Ras exchange factor son of sevenless, Chris A. Karlovich, Laura Bonfini, Linda McCollam, Ronald D. Rogge, Andrea Daga, Michael P. Czech, and Utpal Banerjee

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Divergent mechanisms for homologous desensitization of p21ras by insulin and growth factors, Jes K. Klarlund, Andrew D. Cherniack, and Michael P. Czech

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Functional roles for the pleckstrin and Dbl homology regions in the Ras exchange factor Son-of-sevenless, Linda McCollam, Laura Bonfini, Chris A. Karlovich, Bruce R. Conway, Lynn M. Kozma, Utpal Banerjee, and Michael P. Czech

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Interactions of Cbl with Grb2 and phosphatidylinositol 3'-kinase in activated Jurkat cells, Herman Meisner, Bruce R. Conway, David Alan Hartley, and Michael P. Czech

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Coupling of the proto-oncogene product c-Cbl to the epidermal growth factor receptor, Herman Meisner and Michael P. Czech

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Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii, Gregory J. Pazour, Oleg A. Sineshchekov, and George B. Witman (The Journal of cell biology)

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Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine, Joel Raingeaud, Shashi Gupta, Jeffrey Scott Rogers, Martin Dickens, Jiahuai Han, Richard J. Ulevitch, and Roger J. Davis

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Delineating minimal protein domains and promoter elements for transcriptional activation by lentivirus Tat proteins, Christopher D. Southgate and Michael R. Green

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Phosphorylation of NUMA occurs during nuclear breakdown and not mitotic spindle assembly, Cynthia A. Sparks, Edward G. Fey, Charles A. Vidair, and Stephen J. Doxsey

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Bacterial expression of Scapharca dimeric hemoglobin: a simple model system for investigating protein cooperatively, Candace M. Summerford, Animesh Dev Pardanani, Andrew H. Betts, Anthony R. Poteete, Gianni Colotti, and William E. Royer

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The 78,000 M(r) intermediate chain of Chlamydomonas outer arm dynein isa WD-repeat protein required for arm assembly, Curtis G. Wilkerson, Stephen M. King, Anthony Koutoulis, Gregory J. Pazour, and George B. Witman (The Journal of cell biology)

Publications from 1994

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Regulation by insulin of phosphatidylinositol 3'-kinase bound to alpha- and beta-isoforms of p85 regulatory subunit, Kurt Baltensperger, Lynn M. Kozma, Steven R. Jaspers, and Michael P. Czech

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Single mRNAs visualized by ultrastructural in situ hybridization are principally localized at actin filament intersections in fibroblasts, Gary J. Bassell, Christine M. Powers, Krishan L. Taneja, and Robert H. Singer

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Actin filaments and the spatial positioning of mRNAS, Gary J. Bassell, Krishan L. Taneja, Edward H. Kislauskis, Cynthia L. Sundell, Christine M. Powers, Anthony F. Ross, and Robert H. Singer

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Insulin-sensitive association of GLUT-4 with endocytic clathrin-coated vesicles revealed with the use of brefeldin A, Ranjan Chakrabarti, Joanne M. Buxton, Marguerite Joly, and Silvia Corvera

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Phosphorylation of the Ras nucleotide exchange factor son of sevenless by mitogen-activated protein kinase, Andrew D. Cherniack, Jes K. Klarlund, and Michael P. Czech

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Crystal structure of oxygenated Scapharca dimeric hemoglobin at 1.7-A resolution, Peter J. Condon and William E. Royer

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A double leucine within the GLUT4 glucose transporter COOH-terminal domain functions as an endocytosis signal, Silvia Corvera, Anil Chawla, Ranjan Chakrabarti, Marguerite Joly, Joanne M. Buxton, and Michael P. Czech

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Detection of changes in near-membrane Ca2+ concentration using a novel membrane-associated Ca2+ indicator, Elaine F. Etter, Michael A. Kuhn, and Fredric S. Fay

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Non-syncytium-inducing HIV type 1 isolated from infected individuals replicates in MT-2 cells, Serene Elizabeth Forte, Kevin S. Byron, John L. Sullivan, and Mohan Somasundaran

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The RNA polymerase I transcription factor, upstream binding factor, interacts directly with the TATA box-binding protein, Hyockman Kwon and Michael R. Green

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Synergistic activation of simian immunodeficiency virus and human immunodeficiency virus type 1 transcription by retinoic acid and phorbol ester through an NF-kappa B-independent mechanism, Joseph Walter Maciaszek, David A. Talmage, and Gregory A. Viglianti

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Human transcription factor YY1 represses human immunodeficiency virus type 1 transcription and virion production, David M. Margolis, Mohan Somasundaran, and Michael R. Green

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Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement, Craig L. Peterson, Andrew K. Dingwall, and Matthew P. Scott

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Yeast TAFIIS in a multisubunit complex required for activated transcription, Joseph C. Reese, Lynne Marie Apone, Scott S. Walker, Loree A. Griffin, and Michael R. Green

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Differential intracellular localizations of GDP dissociation inhibitor isoforms. Insulin-dependent redistribution of GDP dissociation inhibitor-2 in 3T3-L1 adipocytes, Assia Shisheva, Joanne M. Buxton, and Michael P. Czech

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Cloning, characterization, and expression of a novel GDP dissociation inhibitor isoform from skeletal muscle, Assia Shisheva, Thomas C. Sudhof, and Michael P. Czech

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Signal transduction by tumor necrosis factor mediated by JNK protein kinases, Hayla Karen Sluss, Tamera Barrett, Benoit Derijard, and Roger J. Davis

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Localization of HIV RNA in mitochondria of infected cells: potential role in cytopathogenicity, Mohan Somasundaran, Maria L. Zapp, Lars K. Beattie, Lizhen Pang, Kevin S. Byron, Gary J. Bassell, John L. Sullivan, and Robert H. Singer

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The native structure of the activated Raf protein kinase is a membrane-bound multi-subunit complex, Markus Wartmann and Roger J. Davis

Publications from 1993

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Redistribution of clathrin-coated vesicle adaptor complexes during adipocytic differentiation of 3T3-L1 cells, Ranjan Chakrabarti, Marguerite Joly, and Silvia Corvera

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Insulin action on the internalization of the GLUT4 glucose transporter in isolated rat adipocytes, Michael P. Czech and Joanne M. Buxton

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Exofacial epitope-tagged glucose transporter chimeras reveal COOH-terminal sequences governing cellular localization, Michael P. Czech, Anil Chawla, Chee-Wai Woon, Joanne M. Buxton, Michal Armoni, Wei Tang, Marguerite Joly, and Silvia Corvera

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The mitogen-activated protein kinase signal transduction pathway, Roger J. Davis

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Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus, Fernando A. Gonzalez, Alpna Seth, David L. Raden, Douglas S. Bowman, Fredric S. Fay, and Roger J. Davis

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Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62, Shashi Gupta, Alpna Seth, and Roger J. Davis

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The ras signaling pathway mimics insulin action on glucose transporter translocation, Lynn M. Kozma, Kurt Baltensperger, Jes K. Klarlund, A Porras, E Santos, and Michael P. Czech

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E1A functions as a coactivator of retinoic acid-dependent retinoic acid receptor-beta 2 promoter activation, Frank A. E. Kruyt, Gert E. Folkers, Albertha J. M. Walhout, Bas-jan M. van der Leede, and Paul T. van der Saag

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The cAMP response element binding protein, CREB, is a potent inhibitor of diverse transcriptional activators, Frederic P. Lemaigre, Christopher I. Ace, and Michael R. Green

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cPLA2 is phosphorylated and activated by MAP kinase, Lih-Ling Lin, Markus Wartmann, Alice Y. Lin, John L. Knop, Alpna Seth, and Roger J. Davis

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sieB and esc genes of Bacteriophage P22: A Dissertation, Koustubh Ranade

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Mitogen-activated protein kinase stimulation by a tyrosine kinase-negative epidermal growth factor receptor, Erica Marie Selva, David L. Raden, and Roger J. Davis

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Cell cycle regulation of the c-Myc transcriptional activation domain, Alpna Seth, Shashi Gupta, and Roger J. Davis

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Assignment of the nuclear mitotic apparatus protein NuMA gene to human chromosome 11q13, Cynthia A. Sparks, Peter Lawrence Bangs, Gerard P. McNeil, Jeanne B. Lawrence, and Edward G. Fey

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Induction of renal adenocarcinoma by a nonmutated erbB oncogene, Cherie A. Taglienti, Barbara F. Banner, Roger J. Davis, and Harriet L. Robinson

Publications from 1992

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Catalysis of serine and tyrosine autophosphorylation by the human insulin receptor, Kurt Baltensperger, Robert E. Lewis, Chee-Wai Woon, Prabhakar Vissavajjhala, Alonzo H. Ross, and Michael P. Czech

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Activation of cell surface glucose transporters measured by photoaffinity labeling of insulin-sensitive 3T3-L1 adipocytes, Scott A. Harrison, Brian M. Clancy, Anna Pessino, and Michael P. Czech

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Glucose transporter oligomeric structure determines transporter function. Reversible redox-dependent interconversions of tetrameric and dimeric GLUT1, Daniel N. Hebert and Anthony Carruthers

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Protein tyrosine kinase activities of the epidermal growth factor receptor and ErbB proteins: correlation of oncogenic activation with altered kinetics, Nandini Nair, Roger J. Davis, and Harriet L. Robinson

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Signal transduction within the nucleus by mitogen-activated protein kinase, Alpna Seth, Fernando A. Gonzalez, Shashi Gupta, David L. Raden, and Roger J. Davis

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Rapid screening of cloned DNA fragments for specific mutations, Steven J. Theroux and Roger J. Davis

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Mutational removal of the major site of serine phosphorylation of the epidermal growth factor receptor causes potentiation of signal transduction: role of receptor down-regulation, Steven J. Theroux, Krista Stanley, Debra A. Campbell, and Roger J. Davis

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Increased oncogenic potential of ErbB is associated with the loss of a COOH-terminal domain serine phosphorylation site, Steven J. Theroux, Cherie A. Taglienti, Nandini Nair, Janice L. Countaway, Harriet L. Robinson, and Roger J. Davis

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Residues in the longitudinal, hydrophobic strip-of-helix relate to terminations and crossings of alpha-helices, Stephen R. Vazquez, Dennis Z. Kuo, Christopher M. Bositis, Larry W. Hardy, Robert A. Lew, and Robert E. Humphreys

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Role of TAR RNA splicing in translational regulation of simian immunodeficiency virus from rhesus macaques, Gregory A. Viglianti, Eric P. Rubinstein, and Karen Lorraine Graves

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Purification and characterization of a cytosolic insulin-stimulated serine kinase from rat liver, Lynn Wolfe, Andrew P. Bradford, Jes K. Klarlund, and Michael P. Czech

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Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit, Meng Zhang, Phillip D. Zamore, Maria Carmo-Fonseca, Angus I. Lamond, and Michael R. Green

Publications from 1991

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Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c-myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase, Elvira Alvarez, Ingrid C. Northwood, Fernando A. Gonzalez, Debra A. Latour, Alpna Seth, Cory Abate, Tom Curran, and Roger J. Davis

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Constitutive phosphorylation of the epidermal growth factor receptor blocks mitogenic signal transduction, Sharon Bowen, Krista Stanley, Erica Marie Selva, and Roger J. Davis

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Extracellular release as the major degradative pathway of the insulin-like growth factor II/mannose 6-phosphate receptor, Kevin B. Clairmont and Michael P. Czech

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Protein synthesis inhibitors activate glucose transport without increasing plasma membrane glucose transporters in 3T3-L1 adipocytes, Brian M. Clancy, Scott A. Harrison, Joanne M. Buxton, and Michael P. Czech

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Acute inhibition of insulin-stimulated glucose transport by the phosphatase inhibitor, okadaic acid, Silvia Corvera, Steven R. Jaspers, and Marguerite Pasceri

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The nuclear matrix: defining structural and functional roles, Edward G. Fey, Peter Lawrence Bangs, Cynthia A. Sparks, and Paul R. Odgren

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Mutational analysis of the cytoplasmic tail of the human transferrin receptor. Identification of a sub-domain that is required for rapid endocytosis, Nuria Girones, Elvira Alvarez, Alpna Seth, I-Mei Lin, Debra A. Latour, and Roger J. Davis

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Identification of substrate recognition determinants for human ERK1 and ERK2 protein kinases, Fernando A. Gonzalez, David L. Raden, and Roger J. Davis

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Evidence that erythroid-type glucose transporter intrinsic activity is modulated by cadmium treatment of mouse 3T3-L1 cells, Scott A. Harrison, Joanne M. Buxton, Brian M. Clancy, and Michael P. Czech

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Suppressed intrinsic catalytic activity of GLUT1 glucose transporters in insulin-sensitive 3T3-L1 adipocytes, Scott A. Harrison, Joanne M. Buxton, and Michael P. Czech

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Enhanced casein kinase II activity in COS-1 cells upon overexpression of either its catalytic or noncatalytic subunit, Robin A. Heller-Harrison and Michael P. Czech

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An insulin-stimulated kemptide kinase purified from rat liver is deactivated by phosphatase 2A, Jes K. Klarlund, Steven R. Jaspers, Nazer Khalaf, Andrew P. Bradford, Thomas B. Miller Jr., and Michael P. Czech

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Isolation and characterization of two growth factor-stimulated protein kinases that phosphorylate the epidermal growth factor receptor at threonine 669, Ingrid C. Northwood, Fernando A. Gonzalez, Markus Wartmann, David L. Raden, and Roger J. Davis

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Evidence that functional erythrocyte-type glucose transporters are oligomers, Anna Pessino, Daniel N. Hebert, Chee-Wai Woon, Scott A. Harrison, Brian M. Clancy, Joanne M. Buxton, Anthony Carruthers, and Michael P. Czech

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A phosphorylation site located in the NH2-terminal domain of c-Myc increases transactivation of gene expression, Alpna Seth, Elvira Alvarez, Shashi Gupta, and Roger J. Davis

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Highly restricted distributions of hydrophobic and charged amino acids in longitudinal quadrants of alpha-helices, Rochelle R. Torgerson, Robert A. Lew, Victor E. Reyes, Larry W. Hardy, and Robert E. Humphreys

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Oligomerization and RNA binding domains of the type 1 human immunodeficiency virus Rev protein: a dual function for an arginine-rich binding motif, Maria L. Zapp, Thomas J. Hope, Tristram G. Parslow, and Michael R. Green

Publications from 1990

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Inhibition of the receptor-mediated endocytosis of diferric transferrin is associated with the covalent modification of the transferrin receptor with palmitic acid, Elvira Alvarez, Nuria Girones, and Roger J. Davis

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The Structure, Function, and Regulation of Insulin-like Growth factor II/Mannose 6-phosphate Receptor Forms: a Thesis, Kevin B. Clairmont

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Insulin injection increases the levels of serum receptors for transferrin and insulin-like growth factor-II/mannose-6-phosphate in intact rats, Kevin B. Clairmont and Michael P. Czech

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Hexose transport stimulation and membrane redistribution of glucose transporter isoforms in response to cholera toxin, dibutyryl cyclic AMP, and insulin in 3T3-L1 adipocytes, Brian M. Clancy and Michael P. Czech

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Insulin stimulates the assembly of cytosolic clathrin onto adipocyte plasma membranes, Silvia Corvera

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Enhanced phosphorylation of a coated vesicle polypeptide in response to insulin stimulation of rat adipocytes, Silvia Corvera and Renold J. Capocasale

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Multisite phosphorylation of the epidermal growth factor receptor. Use of site-directed mutagenesis to examine the role of serine/threonine phosphorylation, Janice L. Countaway, Patricia A. McQuilkin, Nuria Girones, and Roger J. Davis

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Insulin regulation of hexose transport in mouse 3T3-L1 cells expressing the human HepG2 glucose transporter, Scott A. Harrison, Joanne M. Buxton, Brian M. Clancy, and Michael P. Czech

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Insulin action on activity and cell surface disposition of human HepG2 glucose transporters expressed in Chinese hamster ovary cells, Scott A. Harrison, Joanne M. Buxton, Amy L. Helgerson, Richard G. MacDonald, Francis J. Chlapowski, Anthony Carruthers, and Michael P. Czech

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Purification of a novel insulin-stimulated protein kinase from rat liver, Jes K. Klarlund, Andrew P. Bradford, Maria G. Milla, and Michael P. Czech