The Program in Molecular Medicine at the University of Massachusetts Medical School was established in a new research building in 1989. Faculty include both clinical investigators and basic scientists, many of whom focus their research on human disease processes. The laboratory groups and core facilities that compose Molecular Medicine represent many disciplines in the bio-medical sciences: biochemistry and molecular biology, cell biology, medicine, immunology, molecular genetics and microbiology, pediatrics, pharmacology, physiology, genomics, bioinformatics and proteomics. This diverse scientific environment makes the Program an exceptional training ground for graduate students and postdoctoral fellows, who in turn contribute much to its vitality and excitement. This collection showcases publications and presentations written by faculty and researchers of the Program in Molecular Medicine.
Publications from 2001
MKK7 is an essential component of the JNK signal transduction pathway activated by proinflammatory cytokines, Cathy Tournier, Chen Dong, Tod K. Turner, Stephen N. Jones, Richard A. Flavell, and Roger J. Davis
HAT activity is essential for CBP-1-dependent transcription and differentiation in Caenorhabditis elegans, Martin Victor, Yanxia Bei, Frederique Gay, Dominica Calvo, Craig C. Mello, and Yang Shi
Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate, Joseph V. Virbasius, Xi Song, Darcy P. Pomerleau, Yong Zhan, G. Wayne Zhou, and Michael P. Czech
High-throughput yeast two-hybrid assays for large-scale protein interaction mapping, Albertha J. M. Walhout and Marc Vidal
Protein interaction maps for model organisms, Albertha J. M. Walhout and Marc Vidal
Requirement of the JIP1 scaffold protein for stress-induced JNK activation, Alan J. Whitmarsh, Chia-Yi Kuan, Norman J. Kennedy, Nyaya Kelkar, Tarik F. Haydar, John P. Mordes, Michael C. Appel, Aldo A. Rossini, Stephen N. Jones, Richard A. Flavell, Pasko Rakic, and Roger J. Davis
Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56, Meng Zhang and Michael R. Green
The p40phox and p47phox PX domains of NADPH oxidase target cell membranes via direct and indirect recruitment by phosphoinositides, Yong Zhan, Joseph V. Virbasius, Xi Song, Darcy P. Pomerleau, and G. Wayne Zhou
Determinants of the broad recognition of exocytic Rab GTPases by Mss4, Zhongyuan Zhu, Anna M. Delprato, Eric Lee Merithew, and David G. Lambright
A helical turn motif in Mss4 is a critical determinant of Rab binding and nucleotide release, Zhongyuan Zhu, John J. Dumas, Susan E. Lietzke, and David G. Lambright
Construction of centrosomes and spindle poles by molecular motor-driven assembly of protein particles, Wendy Cherie Zimmerman and Stephen J. Doxsey
Publications from 2000
Unusual polymorphisms in human immunodeficiency virus type 1 associated with nonprogressive infection, Louis Alexander, Emma Weiskopf, Thomas C. Greenough, Nathan C. Gaddis, Marcy R. Auerbach, Malcolm H. Malim, Stephen J. O'Brien, Bruce D. Walker, John L. Sullivan, and Ronald C. Desrosiers
Conserved Features of Chromatin Remodeling Enzymes: A Dissertation, Laurie A. Boyer
Functional delineation of three groups of the ATP-dependent family of chromatin remodeling enzymes, Laurie A. Boyer, Colin Logie, Edgar Bonte, Peter B. Becker, Paul A. Wade, Alan P. Wolffe, Carl Wu, Anthony N. Imbalzano, and Craig L. Peterson
Actin-related proteins (Arps): conformational switches for chromatin-remodeling machines, Laurie A. Boyer and Craig L. Peterson
Roles of the histone H2A-H2B dimers and the (H3-H4)(2) tetramer in nucleosome remodeling by the SWI-SNF complex, Laurie A. Boyer, Xiao Shao, Richard H. Ebright, and Craig L. Peterson
c-Jun NH(2)-terminal kinase inhibits targeting of the protein phosphatase calcineurin to NFATc1, Chi-Wing Chow, C Dong, Richard A. Flavell, and Roger J. Davis
JNK1 is required for T cell-mediated immunity against Leishmania major infection, Stephanie L. Constant, Chen Dong, Derek D. Yang, Mark Allen Wysk, Roger J. Davis, and Richard A. Flavell
Cell confluence-dependent remodeling of endothelial membranes mediated by cholesterol, Silvia Corvera, Carlo DiBonaventura, and Howard S. Shpetner
Effect of HIV on thymic function before and after antiretroviral therapy in children, Daniel C. Douek, Richard A. Koup, Richard D. McFarland, John L. Sullivan, and Katherine Luzuriaga
A role for phospholipase D in GLUT4 glucose transporter translocation, Masahiro Emoto, Jes K. Klarlund, Steve B. Waters, Vivian Hu, Joanne M. Buxton, Anil Chawla, and Michael P. Czech
A green fluorescent protein-based reverse two-hybrid system: application to the characterization of large numbers of potential protein-protein interactions, Hideki Endoh, Albertha J. M. Walhout, and Marc Vidal
Molecular determinants that mediate selective activation of p38 MAP kinase isoforms, Herve Enslen, Deborah Marie Brancho, and Roger J. Davis
Genetic requirements for inheritance of RNAi in C. elegans, Alla Grishok, Hiroaki Tabara, and Craig C. Mello
Perinuclear localization and insulin responsiveness of GLUT4 requires cytoskeletal integrity in 3T3-L1 adipocytes, Adilson L. Guilherme, Masahiro Emoto, Joanne M. Buxton, Sahana Bose, Rosanna Sabini, William E. Theurkauf, John D. Leszyk, and Michael P. Czech
c-Jun N-terminal kinase (JNK) repression during the inflammatory response? Just say NO, J. Perry Hall, Eric Lee Merithew, and Roger J. Davis
Snail/slug family of repressors: slowly going into the fast lane of development and cancer, Kirugaval Hemavathy, Shovon Imtiaz Ashraf, and Y. Tony Ip
Human Slug is a repressor that localizes to sites of active transcription, Kirugaval Hemavathy, S C Guru, John E. Harris, J. Don Chen, and Y. Tony Ip
The protein-tyrosine kinase fer associates with signaling complexes containing insulin receptor substrate-1 and phosphatidylinositol 3-kinase, Masanori Iwanishi, Michael P. Czech, and Andrew D. Cherniack
SWI-SNF-mediated nucleosome remodeling: role of histone octamer mobility in the persistence of the remodeled state, Mariela Jaskelioff, Igor M. Gavin, Craig L. Peterson, and Colin Logie
Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3, Nyaya Kelkar, Shashi Gupta, Martin Dickens, and Roger J. Davis
Distinct polyphosphoinositide binding selectivities for pleckstrin homology domains of GRP1-like proteins based on diglycine versus triglycine motifs, Jes K. Klarlund, William Tsiaras, John Holik, Anil Chawla, and Michael P. Czech
The FYVE domain of early endosome antigen 1 is required for both phosphatidylinositol 3-phosphate and Rab5 binding. Critical role of this dual interaction for endosomal localization, Deirdre C. Lawe, Varsha Patki, Robin A. Heller-Harrison, David G. Lambright, and Silvia Corvera
Structural basis of 3-phosphoinositide recognition by pleckstrin homology domains, Susan E. Lietzke, Sahana Bose, Thomas Charles Cronin, Jes K. Klarlund, Anil Chawla, Michael P. Czech, and David G. Lambright
Distinct classes of yeast promoters revealed by differential TAF recruitment, Xiao-Yong Li, Sukesh R. Bhaumik, and Michael R. Green
Early therapy of vertical human immunodeficiency virus type 1 (HIV-1) infection: control of viral replication and absence of persistent HIV-1-specific immune responses, Katherine Luzuriaga, Margaret M. McManus, Michelle D. Catalina, Shane Renee Mayack, Mark E. Sharkey, Mario Stevenson, and John L. Sullivan
Carbon monoxide has anti-inflammatory effects involving the mitogen-activated protein kinase pathway, Leo E. Otterbein, Fritz H. Bach, Jawed Alam, Miguel P. Soares, Hong-Tao Lu, Mark Allen Wysk, Roger J. Davis, Richard A. Flavell, and Augustine M. K. Choi
Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella, Gregory J. Pazour, Bethany L. Dickert, Yvonne Vucica, E. Scott Seeley, Joel L. Rosenbaum, George B. Witman, and Douglas G. Cole
The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative Calpha mRNA expressed specifically in spermatogenic cells, Jovenal T. San Agustin, Curtis G. Wilkerson, and George B. Witman
Persistence of episomal HIV-1 infection intermediates in patients on highly active anti-retroviral therapy, Mark E. Sharkey, Ian Teo, Thomas Greenough, Natalia Sharova, Katherine Luzuriaga, John L. Sullivan, R. Pat Bucy, Leondios G. Kostrikis, Ashley Haase, Claire Veryard, Raul E. Davaro, Sarah H. Cheeseman, Jennifer S. Daly, Carol A. Bova, Richard T. Ellison III, Brian Mady, Kwan Kew Lai, Graeme Moyle, Mark Nelson, Brian Gazzard, Sunil Shaunak, and Mario Stevenson
Actin cytoskeleton: putting a CAP on actin polymerization, Victoria A. Stevenson and William E. Theurkauf
Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway, Cathy Tournier, Patricia M. Hess, Derek D. Yang, Jie Xu, Tod K. Turner, Anjaruwee S. Nimnual, Dafna Bar-Sagi, Stephen N. Jones, Richard A. Flavell, and Roger J. Davis
Light intermediate chain 1 defines a functional subfraction of cytoplasmic dynein which binds to pericentrin, Sharon H. Tynan, Aruna Purohit, Stephen J. Doxsey, and Richard B. Vallee
Yeast two-hybrid systems and protein interaction mapping projects for yeast and worm, Albertha J. M. Walhout, Simon J. Boulton, and Marc Vidal
GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes, Albertha J. M. Walhout, Gary F. Temple, Michael A. Brasch, James L. Hartley, Monique A. Lorson, Sander van den Heuvel, and Marc Vidal
The Role of MKK3 in Mediating Signals to the p38 MAP Kinase Pathway: A Dissertation, Mark Allen Wysk
Structural basis for substrate specificity of protein-tyrosine phosphatase SHP-1, Jian Yang, Zhiliang Cheng, Tian-Qi Niu, Xiaoshan Liang, Zhizhuang Joe Zhao, and G. Wayne Zhou
Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes, Aaron Isadore Young, Jason B. Dictenberg, Aruna Purohit, Richard A. Tuft, and Stephen J. Doxsey
Publications from 1999
The mesoderm determinant snail collaborates with related zinc-finger proteins to control Drosophila neurogenesis, Shovon Imtiaz Ashraf, Xiaodi Hu, John Roote, and Y. Tony Ip
Integration of calcium and cyclic AMP signaling pathways by 14-3-3, Chi-Wing Chow and Roger J. Davis
Requirement for transcription factor NFAT in interleukin-2 expression, Chi-Wing Chow, Mercedes Rincon, and Roger J. Davis
Signaling mechanisms that regulate glucose transport, Michael P. Czech and Silvia Corvera
Structural basis of activation and GTP hydrolysis in Rab proteins, John J. Dumas, Zhongyuan Zhu, Joseph L. Connolly, and David G. Lambright
A novel nuclear export activity in HIV-1 matrix protein required for viral replication, Stefan A. Dupont, Natalia Sharova, Caryn DeHoratius, Ching-man A. Virbasius, Xiaochun Zhu, Alissa G. Bukrinskaya, Mario Stevenson, and Michael R. Green
Role of protein kinase C in signal attenuation following T cell receptor engagement, Belen Fernandez, Michael P. Czech, and Herman Meisner
A role for the yeast SWI/SNF complex in DNA replication, Joan Frances Flanagan and Craig L. Peterson
Centrosome proteins: a major class of autoantigens in scleroderma, Irina Catrinel Gavanescu, Dolores Vazquez-Abad, J. McCauley, Jean-Luc Senecal, and Stephen J. Doxsey
Long-term nonprogressive infection with human immunodeficiency virus type 1 in a hemophilia cohort, Thomas C. Greenough, Doreen B. Brettler, Frank Kirchhoff, Louis Alexander, Ronald C. Desrosiers, Stephen J. O'Brien, Mohan Somasundaran, Katherine Luzuriaga, and John L. Sullivan
Interaction and specificity of Rel-related proteins in regulating Drosophila immunity gene expression, Zhiqiang Han and Y. Tony Ip
An insertional mutant of Chlamydomonas reinhardtii with defective microtubule positioning, Cynthia J. Horst, Douglas J. Fishkind, Gregory J. Pazour, and George B. Witman (Cell motility and the cytoskeleton)
Pre-mRNA splicing of IgM exons M1 and M2 is directed by a juxtaposed splicing enhancer and inhibitor, Julie L. C. Kan and Michael R. Green
Cell cycle-regulated histone acetylation required for expression of the yeast HO gene, Jocelyn E. Krebs, Min-Hao Kuo, C. David Allis, and Craig L. Peterson
ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1, Stephen E. Langille, Varsha Patki, Jes K. Klarlund, Joanne M. Buxton, John Holik, Anil Chawla, Silvia Corvera, and Michael P. Czech
The mammalian homolog of the frog type II selenodeiodinase does not encode a functional enzyme in the rat, Jack L. Leonard, Deborah M. Leonard, Marjorie Safran, Rui Wu, Maria L. Zapp, and Alan P. Farwell
Defective IL-12 production in mitogen-activated protein (MAP) kinase kinase 3 (Mkk3)-deficient mice, Hong-Tao Lu, Derek D. Yang, Mark Allen Wysk, Evelina Gatti, Ira Mellman, Roger J. Davis, and Richard A. Flavell
Toll receptor-mediated Drosophila immune response requires Dif, an NF-kappaB factor, Xiangjun Meng, Bhupinder S. Khanuja, and Y. Tony Ip
LC2, the chlamydomonas homologue of the t complex-encoded protein Tctex2, is essential for outer dynein arm assembly, Gregory J. Pazour, Anthony Koutoulis, Sharon E. Benashski, Bethany L. Dickert, Hong Sheng, Ramila S. Patel-King, Stephen M. King, and George B. Witman
The hepatitis B pX protein promotes dimerization and DNA binding of cellular basic region/leucine zipper proteins by targeting the conserved basic region, Giovanni Perini, Elke Oetjen, and Michael R. Green
Functional interaction between GCN5 and polyamines: a new role for core histone acetylation, Kerri Jeanne Pollard, Michael L. Samuels, Kimberly A. Crowley, Jeffrey C. Hansen, and Craig L. Peterson
Direct interaction of pericentrin with cytoplasmic dynein light intermediate chain contributes to mitotic spindle organization, Aruna Purohit, Sharon H. Tynan, Richard B. Vallee, and Stephen J. Doxsey
WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans, Christian Ernest Rocheleau, Jun Yasuda, Tae Ho Shin, Rueyling Lin, Hitoshi Sawa, Hideyuki Okano, James R. Priess, Roger J. Davis, and Craig C. Mello
Functional analysis of TFIID components, Wu-Cheng Shen, Lynne Marie Apone, Ching-man A. Virbasius, Xiao-Yong Li, M. Monsalve, and Michael R. Green
MOM-4, a MAP kinase kinase kinase-related protein, activates WRM-1/LIT-1 kinase to transduce anterior/posterior polarity signals in C. elegans, Tae Ho Shin, Jun Yasuda, Christian Ernest Rocheleau, Rueyling Lin, Martha C. Soto, Yanxia Bei, Roger J. Davis, and Craig C. Mello
The abnormal gene in X-linked lymphoproliferative syndrome, John L. Sullivan
The rde-1 gene, RNA interference, and transposon silencing in C. elegans, Hiroaki Tabara, Madathia Sarkissian, William G. Kelly, Jamie Fleenor, Alla Grishok, Lisa Timmons, Andrew Z. Fire, and Craig C. Mello
Crystal structure of the nuclear matrix targeting signal of the transcription factor acute myelogenous leukemia-1/polyoma enhancer-binding protein 2alphaB/core binding factor alpha2, Lei Tang, Bo Guo, Amjad Javed, Je-Yong Choi, Scott W. Hiebert, Jane B. Lian, Andre J. Van Wijnen, Janet L. Stein, Gary S. Stein, and G. Wayne Zhou
The MKK7 gene encodes a group of c-Jun NH2-terminal kinase kinases, Cathy Tournier, Alan J. Whitmarsh, Julie Cavanagh, Tamera Barrett, and Roger J. Davis
Profound misregulation of muscle-specific gene expression in facioscapulohumeral muscular dystrophy, Rossella Tupler, Giovanni Perini, Maria Antonietta Pellegrino, and Michael R. Green
Protein interaction mapping in C. elegans using proteins involved in vulval development, Albertha J. M. Walhout, Raffaella Sordella, Xiaowei Lu, James L. Hartley, Gary F. Temple, Michael A. Brasch, Nicolas Thierry-Mieg, and Marc Vidal
A genetic strategy to eliminate self-activator baits prior to high-throughput yeast two-hybrid screens, Albertha J. M. Walhout and Marc Vidal
Requirement of mitogen-activated protein kinase kinase 3 (MKK3) for tumor necrosis factor-induced cytokine expression, Mark Allen Wysk, Derek D. Yang, Hong-Tao Lu, Richard A. Flavell, and Roger J. Davis
The JIP group of mitogen-activated protein kinase scaffold proteins, Jun Yasuda, Alan J. Whitmarsh, Julie Cavanagh, Manoj Sharma, and Roger J. Davis
Centrosome dynamics in living cells, Aaron Isadore Young, Richard A. Tuft, Walter A. Carrington, and Stephen J. Doxsey
Amorphous no longer: the centrosome comes into focus, Wendy Cherie Zimmerman, Cynthia A. Sparks, and Stephen J. Doxsey
Publications from 1998
Transcription sans TBP, Lynne Marie Apone and Michael R. Green
Broad, but not universal, transcriptional requirement for yTAFII17, a histone H3-like TAFII present in TFIID and SAGA, Lynne M. Apone, Ching-man A. Virbasius, Frank C. P. Holstege, Jing Wang, Richard A. Young, and Michael R. Green
Transcriptional control: repression by local chromatin modification, Shovon Imtiaz Ashraf and Y. Tony Ip
Functional analysis of Tpr: identification of nuclear pore complex association and nuclear localization domains and a role in mRNA export, Peter Lawrence Bangs, Brian Burke, Christine M. Powers, Roger W. Craig, Aruna Purohit, and Stephen J. Doxsey
Establishment of a functional human immunodeficiency virus type 1 (HIV-1) reverse transcription complex involves the cytoskeleton, Alissa G. Bukrinskaya, Beda Brichacek, Angela Mann, and Mario Stevenson
Pericentrin and gamma-tubulin form a protein complex and are organized into a novel lattice at the centrosome, Jason B. Dictenburg, Wendy Cherie Zimmerman, Cynthia A. Sparks, Aaron Isadore Young, Charles A. Vidair, Yixian Zheng, Walter A. Carrington, Fredric S. Fay, and Stephen J. Doxsey
Defective T cell differentiation in the absence of Jnk1, Chen Dong, Derek D. Yang, Mark Allen Wysk, Alan J. Whitmarsh, Roger J. Davis, and Richard A. Flavell
Changes in thymic function with age and during the treatment of HIV infection, Daniel C. Douek, Richard D. McFarland, Phillip H. Keiser, Earl A. Gage, Janice M. Massey, Barton F. Haynes, Michael A. Polis, Ashley T. Haase, Mark B. Feinberg, John L. Sullivan, Beth D. Jamieson, Jerome A. Zack, Louis J. Picker, and Richard A. Koup
Mechanism of sodium arsenite-mediated induction of heme oxygenase-1 in hepatoma cells. Role of mitogen-activated protein kinases, Kimberly K. Elbirt, Alan J. Whitmarsh, Roger J. Davis, and Herbert L. Bonkovsky
Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6, Herve Enslen, Joel Raingeaud, and Roger J. Davis
The cytoplasmic zinc finger protein ZPR1 accumulates in the nucleolus of proliferating cells, Zoya Galcheva-Gargova, Laxman Gangwani, Konstantin N. Konstantinov, Monique Mikrut, Steven J. Theroux, Tamar Enoch, and Roger J. Davis
Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells, Laxman Gangwani, Monique Mikrut, Zoya Galcheva-Gargova, and Roger J. Davis
Wilson disease in 1998: genetic, diagnostic and therapeutic aspects, John L. Gollan and Timothy J. Gollan
Stimulation of IRS-1-associated phosphatidylinositol 3-kinase and Akt/protein kinase B but not glucose transport by beta1-integrin signaling in rat adipocytes, Adilson L. Guilherme and Michael P. Czech
Cross-talk between insulin receptor and integrin alpha5 beta1 signaling pathways, Adilson L. Guilherme, Kevin Torres, and Michael P. Czech
A conserved p38 mitogen-activated protein kinase pathway regulates Drosophila immunity gene expression, Zhiqiang Han, Herve Enslen, Xiaodi Hu, Xiangjun Meng, I-Huan Wu, Tamera Barrett, Roger J. Davis, and Y. Tony Ip