The Program in Molecular Medicine at the University of Massachusetts Medical School was established in a new research building in 1989. Faculty include both clinical investigators and basic scientists, many of whom focus their research on human disease processes. The laboratory groups and core facilities that compose Molecular Medicine represent many disciplines in the bio-medical sciences: biochemistry and molecular biology, cell biology, medicine, immunology, molecular genetics and microbiology, pediatrics, pharmacology, physiology, genomics, bioinformatics and proteomics. This diverse scientific environment makes the Program an exceptional training ground for graduate students and postdoctoral fellows, who in turn contribute much to its vitality and excitement. This collection showcases publications and presentations written by faculty and researchers of the Program in Molecular Medicine.

Follow


Publications from 2002

Link

Differential involvement of p38 mitogen-activated protein kinase kinases MKK3 and MKK6 in T-cell apoptosis, Nobuyuki Tanaka, Masahito Kamanaka, Herve Enslen, Chen Dong, Mark Allen Wysk, Roger J. Davis, and Richard A. Flavell

Link

Integrating interactome, phenome, and transcriptome mapping data for the C. elegans germline, Albertha J. M. Walhout, Jerome Reboul, Olena Shtanko, Nicolas Bertin, Philippe Vaglio, Hui Ge, Hongmei Lee, Lynn Doucette-Stamm, Kristin C. Gunsalus, Aaron J. Schetter, Diane G. Morton, Kenneth J. Kemphues, Valerie Reinke, Stuart K. Kim, Fabio Piano, and Marc Vidal

Link

Crystal structure of human protein-tyrosine phosphatase SHP-1, Jian Yang, Lijun Liu, Dandan He, Xi Song, Xiaoshan Liang, Zhizhuang Joe Zhao, and G. Wayne Zhou

Link

Synthesis of pCpCpA-3'-NH-phenylalanine as a ribosomal substrate, Biliang Zhang, Lei Zhang, Lele Sun, and Zhiyong Cui

Publications from 2001

PDF

Snail Protein Family in Drosophila Neurogenesis: a Dissertation, Shovon I. Ashraf

Link

The Snail protein family regulates neuroblast expression of inscuteable and string, genes involved in asymmetry and cell division in Drosophila, Shovon Imtiaz Ashraf and Y. Tony Ip

Link

SAGA is an essential in vivo target of the yeast acidic activator Gal4p, Sukesh R. Bhaumik and Michael R. Green

PDF

G(alpha)11 signaling through ARF6 regulates F-actin mobilization and GLUT4 glucose transporter translocation to the plasma membrane, Avirup Bose, Andrew D. Cherniack, Stephen E. Langille, Sarah M. Nicoloro, Joanne M. Buxton, Jin Gyoon Park, Anil Chawla, and Michael P. Czech

Link

Modulation of different human immunodeficiency virus type 1 Nef functions during progression to AIDS, Silke Carl, Thomas C. Greenough, Mandy Krumbiegel, Michael Greenberg, Jack Skowronski, John L. Sullivan, and Frank Kirchhoff

Link

Differential evolution and stability of epitope-specific CD8(+) T cell responses in EBV infection, Michelle D. Catalina, John L. Sullivan, Katherine R. Bak, and Katherine Luzuriaga

Link

A protein-protein interaction map of the Caenorhabditis elegans 26S proteasome, Anne Davy, Paul Bello, Nicolas Thierry-Mieg, Philippe Vaglio, Joseph Hitti, Lynn Doucette-Stamm, Danielle Thierry-Mieg, Jerome Reboul, Simon J. Boulton, Albertha J. M. Walhout, Olivier Coux, and Marc Vidal

Link

Induction of apoptosis by a secreted lipocalin that is transcriptionally regulated by IL-3 deprivation, Laxminarayana R. Devireddy, Jose G. Teodoro, Fabien A. Richard, and Michael R. Green

PDF

Stress Activated Protein Kinase Regulation of Gene Expression in Apoptotic Neurons: A Dissertation, Gerard S. De Zutter

Link

Pro-apoptotic gene expression mediated by the p38 mitogen-activated protein kinase signal transduction pathway, Gerard S. De Zutter and Roger J. Davis

Link

Multivalent endosome targeting by homodimeric EEA1, John J. Dumas, Eric Lee Merithew, Eathiraj Sudharshan, Deepa Rajamani, Susan J. Hayes, Deirdre C. Lawe, Silvia Corvera, and David G. Lambright

Link

A role for kinesin in insulin-stimulated GLUT4 glucose transporter translocation in 3T3-L1 adipocytes, Masahiro Emoto, Stephen E. Langille, and Michael P. Czech

PDF

The Yeast SWI/SNF Complex Structure and Function: A Dissertation, Joan Frances Flanagan

Link

Molecular basis of T-cell differentiation, Richard A. Flavell, B. Li, Chen Dong, Hong-Tao Lu, Derek D. Yang, Herve Enslen, Cathy Tournier, Alan J. Whitmarsh, Mark Allen Wysk, D. Conze, Mercedes Rincon, and Roger J. Davis

Link

CD4 down-modulation by human immunodeficiency virus type 1 Nef correlates with the efficiency of viral replication and with CD4(+) T-cell depletion in human lymphoid tissue ex vivo, Svetlana Glushakova, Jan Munch, Silke Carl, Thomas C. Greenough, John L. Sullivan, Leonid Margolis, and Frank Kirchhoff

Link

Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing, Alla Grishok, Amy E. Pasquinelli, Darryl Conte Jr., Na Li, Susan Parrish, Ilho Ha, David L. Baillie, Andrew Z. Fire, Gary Ruvkun, and Craig C. Mello

PDF

CPEB phosphorylation and cytoplasmic polyadenylation are catalyzed by the kinase IAK1/Eg2 in maturing mouse oocytes, Rebecca Hodgman, Joyce Tay, Raul Mendez, and Joel D. Richter

Link

A phosphatidylinositol 3-kinase-independent insulin signaling pathway to N-WASP/Arp2/3/F-actin required for GLUT4 glucose transporter recycling, Zhen Y. Jiang, Anil Chawla, Avirup Bose, Michael Way, and Michael P. Czech

Link

Signaling complexes of the FERM domain-containing protein GRSP1 bound to ARF exchange factor GRP1, Jes K. Klarlund, John Holik, Anil Chawla, Jin Gyoon Park, Joanne M. Buxton, and Michael P. Czech

Link

Sequential roles for phosphatidylinositol 3-phosphate and Rab5 in tethering and fusion of early endosomes via their interaction with EEA1, Deirdre C. Lawe, Anil Chawla, Eric Lee Merithew, John J. Dumas, Walter A. Carrington, Kevin E. Fogarty, Lawrence M. Lifshitz, Richard A. Tuft, David G. Lambright, and Silvia Corvera

Link

Structural plasticity of an invariant hydrophobic triad in the switch regions of Rab GTPases is a determinant of effector recognition, Eric Lee Merithew, Scott Hatherly, John J. Dumas, Deirdre C. Lawe, Robin A. Heller-Harrison, and David G. Lambright

Link

Nevirapine pharmacokinetics in pregnant women and in their infants after in utero exposure, Mark Mirochnick, Sue Siminski, Terry Fenton, Miladi Lugo, and John L. Sullivan

Link

Abnormal T cell receptor signal transduction of CD4 Th cells in X-linked lymphoproliferative syndrome, Hiroyuki Nakamura, Jodi Zarycki, John L. Sullivan, and Jae U. Jung

Link

The Drosophila Toll-9 activates a constitutive antimicrobial defense, James Y. Ooi, Yoshimasa Yagi, Xiaodi Hu, and Y. Tony Ip

Link

PYK2 as a mediator of endothelin-1/G alpha 11 signaling to GLUT4 glucose transporters, Jin Gyoon Park, Avirup Bose, John D. Leszyk, and Michael P. Czech

PDF

Insulin action on GLUT4 traffic visualized in single 3T3-l1 adipocytes by using ultra-fast microscopy, Varsha Patki, Joanne M. Buxton, Anil Chawla, Lawrence M. Lifshitz, Kevin E. Fogarty, Walter A. Carrington, Richard A. Tuft, and Silvia Corvera

Link

Forward and reverse genetic analysis of microtubule motors in Chlamydomonas, Gregory J. Pazour and George B. Witman (Methods (San Diego, Calif.))

PDF

Centrosome defects can account for cellular and genetic changes that characterize prostate cancer progression, German A. Pihan, Aruna Purohit, Jan Wallace, Raji Malhotra, Lance A. Liotta, and Stephen J. Doxsey

Link

Sequence-specific interaction between HIV-1 matrix protein and viral genomic RNA revealed by in vitro genetic selection, Aruna Purohit, Stefan A. Dupont, Mario Stevenson, and Michael R. Green

Link

Differential expression of the C(s) and Calpha1 isoforms of the catalytic subunit of cyclic 3',5'-adenosine monophosphate-dependent protein kinase testicular cells, Jovenal T. San Agustin and George B. Witman

Link

Infrequent detection of HIV-1-specific, but not cytomegalovirus-specific, CD8(+) T cell responses in young HIV-1-infected infants, Zachary Aaron Scott, Ellen G. Chadwick, Laura L. Gibson, Michelle D. Catalina, Margaret M. McManus, Ram Yogev, Paul Palumbo, John L. Sullivan, Paula Britto, Hannah Gay, and Katherine Luzuriaga

Link

Real-time visualization of processive myosin 5a-mediated vesicle movement in living astrocytes, Stanley J. Stachelek, Richard A. Tuft, Lawrence M. Lifschitz, Deborah Leonard, Alan P. Farwell, and Jack L. Leonard

Link

Centrosomes and the Scrambled protein coordinate microtubule-independent actin reorganization, Victoria A. Stevenson, Joseph Kramer, Jesse Kuhn, and William E. Theurkauf

Link

MKK7 is an essential component of the JNK signal transduction pathway activated by proinflammatory cytokines, Cathy Tournier, Chen Dong, Tod K. Turner, Stephen N. Jones, Richard A. Flavell, and Roger J. Davis

Link

HAT activity is essential for CBP-1-dependent transcription and differentiation in Caenorhabditis elegans, Martin Victor, Yanxia Bei, Frederique Gay, Dominica Calvo, Craig C. Mello, and Yang Shi

Link

Activation of the Akt-related cytokine-independent survival kinase requires interaction of its phox domain with endosomal phosphatidylinositol 3-phosphate, Joseph V. Virbasius, Xi Song, Darcy P. Pomerleau, Yong Zhan, G. Wayne Zhou, and Michael P. Czech

Link

High-throughput yeast two-hybrid assays for large-scale protein interaction mapping, Albertha J. M. Walhout and Marc Vidal

Link

Protein interaction maps for model organisms, Albertha J. M. Walhout and Marc Vidal

Link

Requirement of the JIP1 scaffold protein for stress-induced JNK activation, Alan J. Whitmarsh, Chia-Yi Kuan, Norman J. Kennedy, Nyaya Kelkar, Tarik F. Haydar, John P. Mordes, Michael C. Appel, Aldo A. Rossini, Stephen N. Jones, Richard A. Flavell, Pasko Rakic, and Roger J. Davis

Link

Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56, Meng Zhang and Michael R. Green

Link

The p40phox and p47phox PX domains of NADPH oxidase target cell membranes via direct and indirect recruitment by phosphoinositides, Yong Zhan, Joseph V. Virbasius, Xi Song, Darcy P. Pomerleau, and G. Wayne Zhou

Link

Determinants of the broad recognition of exocytic Rab GTPases by Mss4, Zhongyuan Zhu, Anna M. Delprato, Eric Lee Merithew, and David G. Lambright

Link

A helical turn motif in Mss4 is a critical determinant of Rab binding and nucleotide release, Zhongyuan Zhu, John J. Dumas, Susan E. Lietzke, and David G. Lambright

Link

Construction of centrosomes and spindle poles by molecular motor-driven assembly of protein particles, Wendy Cherie Zimmerman and Stephen J. Doxsey

Publications from 2000

Link

Unusual polymorphisms in human immunodeficiency virus type 1 associated with nonprogressive infection, Louis Alexander, Emma Weiskopf, Thomas C. Greenough, Nathan C. Gaddis, Marcy R. Auerbach, Malcolm H. Malim, Stephen J. O'Brien, Bruce D. Walker, John L. Sullivan, and Ronald C. Desrosiers

PDF

Conserved Features of Chromatin Remodeling Enzymes: A Dissertation, Laurie A. Boyer

Link

Functional delineation of three groups of the ATP-dependent family of chromatin remodeling enzymes, Laurie A. Boyer, Colin Logie, Edgar Bonte, Peter B. Becker, Paul A. Wade, Alan P. Wolffe, Carl Wu, Anthony N. Imbalzano, and Craig L. Peterson

Link

Actin-related proteins (Arps): conformational switches for chromatin-remodeling machines, Laurie A. Boyer and Craig L. Peterson

Link

Roles of the histone H2A-H2B dimers and the (H3-H4)(2) tetramer in nucleosome remodeling by the SWI-SNF complex, Laurie A. Boyer, Xiao Shao, Richard H. Ebright, and Craig L. Peterson

PDF

c-Jun NH(2)-terminal kinase inhibits targeting of the protein phosphatase calcineurin to NFATc1, Chi-Wing Chow, C Dong, Richard A. Flavell, and Roger J. Davis

Link

JNK1 is required for T cell-mediated immunity against Leishmania major infection, Stephanie L. Constant, Chen Dong, Derek D. Yang, Mark Allen Wysk, Roger J. Davis, and Richard A. Flavell

Link

Cell confluence-dependent remodeling of endothelial membranes mediated by cholesterol, Silvia Corvera, Carlo DiBonaventura, and Howard S. Shpetner

Link

Effect of HIV on thymic function before and after antiretroviral therapy in children, Daniel C. Douek, Richard A. Koup, Richard D. McFarland, John L. Sullivan, and Katherine Luzuriaga

Link

A role for phospholipase D in GLUT4 glucose transporter translocation, Masahiro Emoto, Jes K. Klarlund, Steve B. Waters, Vivian Hu, Joanne M. Buxton, Anil Chawla, and Michael P. Czech

Link

A green fluorescent protein-based reverse two-hybrid system: application to the characterization of large numbers of potential protein-protein interactions, Hideki Endoh, Albertha J. M. Walhout, and Marc Vidal

Link

Molecular determinants that mediate selective activation of p38 MAP kinase isoforms, Herve Enslen, Deborah Marie Brancho, and Roger J. Davis

Link

Genetic requirements for inheritance of RNAi in C. elegans, Alla Grishok, Hiroaki Tabara, and Craig C. Mello

Link

Perinuclear localization and insulin responsiveness of GLUT4 requires cytoskeletal integrity in 3T3-L1 adipocytes, Adilson L. Guilherme, Masahiro Emoto, Joanne M. Buxton, Sahana Bose, Rosanna Sabini, William E. Theurkauf, John D. Leszyk, and Michael P. Czech

Link

c-Jun N-terminal kinase (JNK) repression during the inflammatory response? Just say NO, J. Perry Hall, Eric Lee Merithew, and Roger J. Davis

Link

Snail/slug family of repressors: slowly going into the fast lane of development and cancer, Kirugaval Hemavathy, Shovon Imtiaz Ashraf, and Y. Tony Ip

PDF

Human Slug is a repressor that localizes to sites of active transcription, Kirugaval Hemavathy, S C Guru, John E. Harris, J. Don Chen, and Y. Tony Ip

Link

The protein-tyrosine kinase fer associates with signaling complexes containing insulin receptor substrate-1 and phosphatidylinositol 3-kinase, Masanori Iwanishi, Michael P. Czech, and Andrew D. Cherniack

Link

SWI-SNF-mediated nucleosome remodeling: role of histone octamer mobility in the persistence of the remodeled state, Mariela Jaskelioff, Igor M. Gavin, Craig L. Peterson, and Colin Logie

PDF

Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3, Nyaya Kelkar, Shashi Gupta, Martin Dickens, and Roger J. Davis

Link

Distinct polyphosphoinositide binding selectivities for pleckstrin homology domains of GRP1-like proteins based on diglycine versus triglycine motifs, Jes K. Klarlund, William Tsiaras, John Holik, Anil Chawla, and Michael P. Czech

Link

The FYVE domain of early endosome antigen 1 is required for both phosphatidylinositol 3-phosphate and Rab5 binding. Critical role of this dual interaction for endosomal localization, Deirdre C. Lawe, Varsha Patki, Robin A. Heller-Harrison, David G. Lambright, and Silvia Corvera

Link

Structural basis of 3-phosphoinositide recognition by pleckstrin homology domains, Susan E. Lietzke, Sahana Bose, Thomas Charles Cronin, Jes K. Klarlund, Anil Chawla, Michael P. Czech, and David G. Lambright

Link

Distinct classes of yeast promoters revealed by differential TAF recruitment, Xiao-Yong Li, Sukesh R. Bhaumik, and Michael R. Green

PDF

Early therapy of vertical human immunodeficiency virus type 1 (HIV-1) infection: control of viral replication and absence of persistent HIV-1-specific immune responses, Katherine Luzuriaga, Margaret M. McManus, Michelle D. Catalina, Shane Renee Mayack, Mark E. Sharkey, Mario Stevenson, and John L. Sullivan

Link

Carbon monoxide has anti-inflammatory effects involving the mitogen-activated protein kinase pathway, Leo E. Otterbein, Fritz H. Bach, Jawed Alam, Miguel P. Soares, Hong-Tao Lu, Mark Allen Wysk, Roger J. Davis, Richard A. Flavell, and Augustine M. K. Choi

PDF

Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella, Gregory J. Pazour, Bethany L. Dickert, Yvonne Vucica, E. Scott Seeley, Joel L. Rosenbaum, George B. Witman, and Douglas G. Cole

PDF

The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative Calpha mRNA expressed specifically in spermatogenic cells, Jovenal T. San Agustin, Curtis G. Wilkerson, and George B. Witman

Link

Persistence of episomal HIV-1 infection intermediates in patients on highly active anti-retroviral therapy, Mark E. Sharkey, Ian Teo, Thomas Greenough, Natalia Sharova, Katherine Luzuriaga, John L. Sullivan, R. Pat Bucy, Leondios G. Kostrikis, Ashley Haase, Claire Veryard, Raul E. Davaro, Sarah H. Cheeseman, Jennifer S. Daly, Carol A. Bova, Richard T. Ellison III, Brian Mady, Kwan Kew Lai, Graeme Moyle, Mark Nelson, Brian Gazzard, Sunil Shaunak, and Mario Stevenson

Link

Actin cytoskeleton: putting a CAP on actin polymerization, Victoria A. Stevenson and William E. Theurkauf

Link

Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway, Cathy Tournier, Patricia M. Hess, Derek D. Yang, Jie Xu, Tod K. Turner, Anjaruwee S. Nimnual, Dafna Bar-Sagi, Stephen N. Jones, Richard A. Flavell, and Roger J. Davis

Link

Light intermediate chain 1 defines a functional subfraction of cytoplasmic dynein which binds to pericentrin, Sharon H. Tynan, Aruna Purohit, Stephen J. Doxsey, and Richard B. Vallee

Link

Yeast two-hybrid systems and protein interaction mapping projects for yeast and worm, Albertha J. M. Walhout, Simon J. Boulton, and Marc Vidal

Link

GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes, Albertha J. M. Walhout, Gary F. Temple, Michael A. Brasch, James L. Hartley, Monique A. Lorson, Sander van den Heuvel, and Marc Vidal

PDF

The Role of MKK3 in Mediating Signals to the p38 MAP Kinase Pathway: A Dissertation, Mark Allen Wysk

Link

Structural basis for substrate specificity of protein-tyrosine phosphatase SHP-1, Jian Yang, Zhiliang Cheng, Tian-Qi Niu, Xiaoshan Liang, Zhizhuang Joe Zhao, and G. Wayne Zhou

Link

Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes, Aaron Isadore Young, Jason B. Dictenberg, Aruna Purohit, Richard A. Tuft, and Stephen J. Doxsey

Publications from 1999

Link

The mesoderm determinant snail collaborates with related zinc-finger proteins to control Drosophila neurogenesis, Shovon Imtiaz Ashraf, Xiaodi Hu, John Roote, and Y. Tony Ip

PDF

Integration of calcium and cyclic AMP signaling pathways by 14-3-3, Chi-Wing Chow and Roger J. Davis

PDF

Requirement for transcription factor NFAT in interleukin-2 expression, Chi-Wing Chow, Mercedes Rincon, and Roger J. Davis

Link

Signaling mechanisms that regulate glucose transport, Michael P. Czech and Silvia Corvera

Link

Structural basis of activation and GTP hydrolysis in Rab proteins, John J. Dumas, Zhongyuan Zhu, Joseph L. Connolly, and David G. Lambright

Link

A novel nuclear export activity in HIV-1 matrix protein required for viral replication, Stefan A. Dupont, Natalia Sharova, Caryn DeHoratius, Ching-man A. Virbasius, Xiaochun Zhu, Alissa G. Bukrinskaya, Mario Stevenson, and Michael R. Green

Link

Role of protein kinase C in signal attenuation following T cell receptor engagement, Belen Fernandez, Michael P. Czech, and Herman Meisner

PDF

A role for the yeast SWI/SNF complex in DNA replication, Joan Frances Flanagan and Craig L. Peterson

Link

Centrosome proteins: a major class of autoantigens in scleroderma, Irina Catrinel Gavanescu, Dolores Vazquez-Abad, J. McCauley, Jean-Luc Senecal, and Stephen J. Doxsey

Link

Long-term nonprogressive infection with human immunodeficiency virus type 1 in a hemophilia cohort, Thomas C. Greenough, Doreen B. Brettler, Frank Kirchhoff, Louis Alexander, Ronald C. Desrosiers, Stephen J. O'Brien, Mohan Somasundaran, Katherine Luzuriaga, and John L. Sullivan

Link

Interaction and specificity of Rel-related proteins in regulating Drosophila immunity gene expression, Zhiqiang Han and Y. Tony Ip

Link

An insertional mutant of Chlamydomonas reinhardtii with defective microtubule positioning, Cynthia J. Horst, Douglas J. Fishkind, Gregory J. Pazour, and George B. Witman (Cell motility and the cytoskeleton)

Link

Pre-mRNA splicing of IgM exons M1 and M2 is directed by a juxtaposed splicing enhancer and inhibitor, Julie L. C. Kan and Michael R. Green

Link

Cell cycle-regulated histone acetylation required for expression of the yeast HO gene, Jocelyn E. Krebs, Min-Hao Kuo, C. David Allis, and Craig L. Peterson

Link

ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1, Stephen E. Langille, Varsha Patki, Jes K. Klarlund, Joanne M. Buxton, John Holik, Anil Chawla, Silvia Corvera, and Michael P. Czech