The Program in Molecular Medicine at the University of Massachusetts Medical School was established in a new research building in 1989. Faculty include both clinical investigators and basic scientists, many of whom focus their research on human disease processes. The laboratory groups and core facilities that compose Molecular Medicine represent many disciplines in the bio-medical sciences: biochemistry and molecular biology, cell biology, medicine, immunology, molecular genetics and microbiology, pediatrics, pharmacology, physiology, genomics, bioinformatics and proteomics. This diverse scientific environment makes the Program an exceptional training ground for graduate students and postdoctoral fellows, who in turn contribute much to its vitality and excitement. This collection showcases publications and presentations written by faculty and researchers of the Program in Molecular Medicine.
Publications from 2004
JNK potentiates TNF-stimulated necrosis by increasing the production of cytotoxic reactive oxygen species, Juan-Jose Ventura, Patricia C. Cogswell, Richard A. Flavell, Albert S. Baldwin Jr., and Roger J. Davis
DNA instructed displacement of histones H2A and H2B at an inducible promoter, Guillermo P. Vicent, A. Silvina Nacht, Corey Lewis Smith, Craig L. Peterson, Stefan Dimitrov, and Miguel Beato
Novel role for a sterol response element binding protein in directing spermatogenic cell-specific gene expression, Hong Wang, Jovenal T. San Agustin, George B. Witman, and Daniel L. Kilpatrick
Regulation of muscle fiber type and running endurance by PPARdelta, Yong-Xu Wang, Chung-Li Zhang, Ruth T. Yu, Helen K. Cho, Michael C. Nelson, Corinne R. Bayuga-Ocampo, Jungyeob Ham, Heonjoong Kang, and Ronald M. Evans
The c-Jun NH2-terminal kinase is essential for epidermal growth factor expression during epidermal morphogenesis, Claire R. Weston, Anthony Wong, J. Perry Hall, Mary E. P. Goad, Richard A. Flavell, and Roger J. Davis
Mitochondrial remodeling in adipose tissue associated with obesity and treatment with rosiglitazone, Leanne Wilson-Fritch, Sarah M. Nicoloro, My T. Chouinard, Mitchell A. Lazar, Patricia C. Chui, John D. Leszyk, Juerg R. Straubhaar, Michael P. Czech, and Silvia Corvera
Mother-to-child transmission of HIV-1: strong association with certain maternal HLA-B alleles independent of viral load implicates innate immune mechanisms, Robert Winchester, Jane Pitt, Manhattan Charurat, Laurence S. Magder, Harald H. H. Goring, Alan Landay, Jennifer S. Read, William T. Shearer, Edward Handelsman, Katherine Luzuriaga, George V. Hillyer, and William A. Blattner
Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase, Maureen Wirschell, Gregory J. Pazour, Akinori Yoda, Masafumi Hirono, Ritsu Kamiya, and George B. Witman
Compartment-specific perturbation of protein handling activates genes encoding mitochondrial chaperones, Takunari Yoneda, Cristina Benedetti, Fumihiko Urano, Scott G. Clark, Heather P. Harding, and David Ron
Quantitative signature for architectural organization of regulatory factors using intranuclear informatics, Daniel W. Young, Sayyed K. Zaidi, Paul S. Furcinitti, Amjad Javed, Andre J. Van Wijnen, Janet L. Stein, Jane B. Lian, and Gary S. Stein
Association of Pericentrin with the γ Tubulin Ring Complex: a Dissertation, Wendy Cherie Zimmerman
Mitosis-specific anchoring of gamma tubulin complexes by pericentrin controls spindle organization and mitotic entry, Wendy Cherie Zimmerman, James Sillibourne, Jack Rosa, and Stephen J. Doxsey
Publications from 2003
Mechanism of p38 MAP kinase activation in vivo, Deborah Marie Brancho, Nobuyuki Tanaka, Anja Jaeschke, Juan-Jose Ventura, Nyaya Kelkar, Yoshinori Tanaka, Masanao Kyuuma, Toshikazu Takeshita, Richard A. Flavell, and Roger J. Davis
Regulation of the SHP-2 tyrosine phosphatase by a novel cholesterol- and cell confluence-dependent mechanism, Alison Burkart, Babak Samii, Silvia Corvera, and Howard S. Shpetner
DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC, Diane M. Casey, Kazuo Inaba, Gregory J. Pazour, Saeko Takada, Ken-ichi Wakabayashi, Curtis G. Wilkerson, Ritsu Kamiya, and George B. Witman
Insulin's expanding control of forkheads, Michael P. Czech
Transcriptional program of apoptosis induction following interleukin 2 deprivation: identification of RC3, a calcium/calmodulin binding protein, as a novel proapoptotic factor, Laxminarayana R. Devireddy and Michael R. Green
Membrane recognition and targeting by lipid-binding domains, Jonathan P. DiNitto, Thomas Charles Cronin, and David G. Lambright
Hepatitis C virus core and nonstructural protein 3 proteins induce pro- and anti-inflammatory cytokines and inhibit dendritic cell differentiation, Angela Dolganiuc, Karen Kodys, Andrea Kopasz, Christopher Marshall, Twan Do, Laszlo Romics, Pranoti Mandrekar, Maria L. Zapp, and Gyongyi Szabo
Chromatin remodeling activities act on UV-damaged nucleosomes and modulate DNA damage accessibility to photolyase, Helene Gaillard, Daniel J. Fitzgerald, Corey Lewis Smith, Craig L. Peterson, Timothy J. Richmond, and Fritz Thoma
The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly, Rita Gandhi, Silvia Bonaccorsi, Diana Wentworth, Stephen J. Doxsey, Maurizio Gatti, and Andrea J. Pereira
Integrating 'omic' information: a bridge between genomics and systems biology, Hui Ge, Albertha J. M. Walhout, and Marc Vidal
A novel human protein of the maternal centriole is required for the final stages of cytokinesis and entry into S phase, Adam Scott Gromley, Agata Jurczyk, James Sillibourne, Ensar Halilovic, Mette M. Mogensen, Irina Groisman, Maureen Blomberg, and Stephen J. Doxsey
EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton, Adilson L. Guilherme, Neil A. Soriano, Sahana Bose, John Holik, Avirup Bose, Darcy P. Pomerleau, Paul S. Furcinitti, John D. Leszyk, Silvia Corvera, and Michael P. Czech
Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B, Zhenyu Gu, Xiaonan Zhu, Shaowei Ni, Haimeng Zhou, and Zhiguo Su
The Molecular Mechanisms of T Cell Clonal Anergy: A Dissertation, John E. Harris
Structural basis for endosomal targeting by FYVE domains, Akira Hayakawa, Susan J. Hayes, Deirdre C. Lawe, Eathiraj Sudharshan, Richard A. Tuft, Kevin E. Fogarty, David Lambright, and Silvia Corvera
Role of c-Jun NH-terminal Kinase in Bcr/Abl Induced Cell Transformation: a dissertation, Patricia M. Hess
Alterations in HIV-1 LTR promoter activity during AIDS progression, Kirsten Hiebenthal-Millow, Thomas C. Greenough, Doreen B. Bretttler, Michael Schindler, Steffen Wildum, John L. Sullivan, and Frank Kirchhoff
Facilitation of dendritic mRNA transport by CPEB, Yi-Shuian Huang, John H. Carson, Elisa Barbarese, and Joel D. Richter
Mechanistic Analysis of Chromatin Remodeling Enzymes: a Dissertation, Mariela Jaskelioff
Chromatin and transcription: histones continue to make their marks, Mariela Jaskelioff and Craig L. Peterson
Rad54p is a chromatin remodeling enzyme required for heteroduplex DNA joint formation with chromatin, Mariela Jaskelioff, Stephen Van Komen, Jocelyn E. Krebs, Patrick Sung, and Craig L. Peterson
Insulin signaling through Akt/protein kinase B analyzed by small interfering RNA-mediated gene silencing, Zhen Y. Jiang, Qiong L. Zhou, Kerri A. Coleman, My T. Chouinard, Queta Boese, and Michael P. Czech
Morphogenesis of the telencephalic commissure requires scaffold protein JNK-interacting protein 3 (JIP3), Nyaya Kelkar, Marie Helene Delmotte, Claire R. Weston, Tamera Barrett, Barbara J. Sheppard, Richard A. Flavell, and Roger J. Davis
Role of JNK in tumor development, Norman J. Kennedy and Roger J. Davis
Suppression of Ras-stimulated transformation by the JNK signal transduction pathway, Norman J. Kennedy, Hayla Karen Sluss, Stephen N. Jones, Dafna Bar-Sagi, Richard A. Flavell, and Roger J. Davis
JunD mediates survival signaling by the JNK signal transduction pathway, Jennifer A. Lamb, Juan-Jose Ventura, Patricia M. Hess, Richard A. Flavell, and Roger J. Davis
Essential role of Ca2+/calmodulin in Early Endosome Antigen-1 localization, Deirdre C. Lawe, Nachida Sitouah, Susan Hayes, Anil Chawla, Joseph V. Virbasius, Richard A. Tuft, Kevin E. Fogarty, Lawrence M. Lifshitz, David G. Lambright, and Silvia Corvera
The death domain kinase RIP1 is essential for tumor necrosis factor alpha signaling to p38 mitogen-activated protein kinase, Thomas H. Lee, Qiaojia Huang, Sarah R. Oikemus, Jennifer Shank, Juan-Jose Ventura, Nicole Cusson, Richard R. Vaillancourt, Bing Su, Roger J. Davis, and Michelle Kelliher
JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis, Kui Lei and Roger J. Davis
Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus, Wenhui Li, Michael J. Moore, Natalya Vasilieva, Jianhua Sui, Swee Kee Wong, Michael A. Berne, Mohan Somasundaran, John L. Sullivan, Katherine Luzuriaga, Thomas C. Greenough, Hyeryun Choe, and Michael Farzan
Predose infant nevirapine concentration with the two-dose intrapartum neonatal nevirapine regimen: association with timing of maternal intrapartum nevirapine dose, Mark Mirochnick, Alejandro Dorenbaum, Suzette Blanchard, Coleen K. Cunningham, Richard D. Gelber, Lynne M. Mofenson, Mary Culnane, and John L. Sullivan
A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex, Sanjay C. Panchal, Donald A. Kaiser, Eduardo M. Torres, Thomas D. Pollard, and Michael K. Rosen
Ian4 is required for mitochondrial integrity and T cell survival, Malini Pandarpurkar, Leanne Wilson-Fritch, Silvia Corvera, Helle Markholst, Lars Hornum, Dale L. Greiner, John P. Mordes, Aldo A. Rossini, and Rita Bortell
The vertebrate primary cilium is a sensory organelle, Gregory J. Pazour and George B. Witman (Current opinion in cell biology)
The Genetic Basis of Resistance to Transplantation Tolerance Induced by Costimulation Blockade in NOD Mice: a Dissertation, Todd Pearson
Genetic disassociation of autoimmunity and resistance to costimulation blockade-induced transplantation tolerance in nonobese diabetic mice, Todd Pearson, Thomas G. Markees, David V. Serreze, Melissa A. Pierce, Michele P. Marron, Linda S. Wicker, Laurence B. Peterson, Leonard D. Shultz, John P. Mordes, Aldo A. Rossini, and Dale L. Greiner
TRF3, a TATA-box-binding protein-related factor, is vertebrate-specific and widely expressed, Stephan P. Persengiev, Xiaochun Zhu, Bharat L. Dixit, Glenn A. Maston, Ellen L. W. Kittler, and Michael R. Green
Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs), Stephan P. Persengiev, Xiaochun Zhu, and Michael R. Green
Centrosome abnormalities and chromosome instability occur together in pre-invasive carcinomas, German A. Pihan, Jan Wallace, Yening Zhou, and Stephen J. Doxsey
T Cell Immunity and HIV-1 Replication in Vertically-Infected Infants and Children: A Dissertation, Zachary Aaron Scott
HIV-1 replication increases HIV-specific CD4+ T cell frequencies but limits proliferative capacity in chronically infected children, Zachary Aaron Scott, Coreen Michele Beaumier, Mark E. Sharkey, Mario Stevenson, and Katherine Luzuriaga
Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules, Sabina Semiz, Jin Gyoon Park, Sarah M. Nicoloro, Paul S. Furcinitti, Chuanyou Zhang, Anil Chawla, John D. Leszyk, and Michael P. Czech
CD40-CD154 Blockade Facilitates Induction of Allogeneic Hematopoietic Chimerism and Transplantation Tolerance: A Dissertation, Edward Seung
Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly, Wu-Cheng Shen, Sukesh R. Bhaumik, Helen C. Causton, Itamar Simon, Xiaochun Zhu, Ezra G. Jennings, Tseng-Hsing Wang, Richard A. Young, and Michael R. Green
A native peptide ligation strategy for deciphering nucleosomal histone modifications, Michael Shogren-Knaak, Christopher J. Fry, and Craig L. Peterson
Structural analysis of the yeast SWI/SNF chromatin remodeling complex, Corey Lewis Smith, Rachel A. Horowitz-Scherer, Joan Frances Flanagan, Christopher L. Woodcock, and Craig L. Peterson
Coupling tandem affinity purification and quantitative tyrosine iodination to determine subunit stoichiometry of protein complexes, Corey Lewis Smith and Craig L. Peterson
Developments in basic science research, Mario Stevenson
Prevention of mother-to-child transmission of HIV--what next, John L. Sullivan
Peptidyltransfer Reaction Catalyzed by the Ribosome and the Ribozyme: a Dissertation, Lele Sun
Regulated CPEB phosphorylation during meiotic progression suggests a mechanism for temporal control of maternal mRNA translation, Joyce Tay, Rebecca Hodgman, Madathia Sarkissian, and Joel D. Richter
Contingent phosphorylation/dephosphorylation provides a mechanism of molecular memory in WASP, Eduardo M. Torres and Michael K. Rosen
MEP-1 and a homolog of the NURD complex component Mi-2 act together to maintain germline-soma distinctions in C. elegans, Yingdee Unhavaithaya, Tae Ho Shin, Nicholas B. Miliaras, Jungsoon Lee, Tomoko Oyama, and Craig C. Mello
c-Jun NH(2)-terminal kinase is essential for the regulation of AP-1 by tumor necrosis factor, Juan-Jose Ventura, Norman J. Kennedy, Jennifer A. Lamb, Richard A. Flavell, and Roger J. Davis
JNK initiates a cytokine cascade that causes Pax2 expression and closure of the optic fissure, Claire R. Weston, Anthony Wong, J. Perry Hall, Mary E. P. Goad, Richard A. Flavell, and Roger J. Davis
Identification of a subset of human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus strains able to exploit an alternative coreceptor on untransformed human brain and lymphoid cells, Samantha J. Willey, Jacqueline D. Reeves, Richard Hudson, Koichi Miyake, Nathalie Dejucq, Dominique Schols, Erik De Clercq, Jeanne E. Bell, Aine McKnight, and Paul R. Clapham
Mitochondrial biogenesis and remodeling during adipogenesis and in response to the insulin sensitizer rosiglitazone, Leanne Wilson-Fritch, Alison Burkart, Gregory Bell, Karen Mendelson, John D. Leszyk, Sarah M. Nicoloro, Michael P. Czech, and Silvia Corvera
Publications from 2002
SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C. elegans embryos, Yanxia Bei, Jennifer Hogan, Laura A. Berkowitz, Martha C. Soto, Christian Ernest Rocheleau, Ka Ming Pang, John J. Collins, and Craig C. Mello
Differential requirement of SAGA components for recruitment of TATA-box-binding protein to promoters in vivo, Sukesh R. Bhaumik and Michael R. Green
Essential role for the SANT domain in the functioning of multiple chromatin remodeling enzymes, Laurie A. Boyer, Michael R. Langer, Kimberly A. Crowley, Song Tan, John M. Denu, and Craig L. Peterson
Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA, Brent D. Brower-Toland, Corey Lewis Smith, Richard C. Yeh, John T. Lis, Craig L. Peterson, and Michelle D. Wang
Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturation, Quiping Cao and Joel D. Richter
Phenotypic and functional heterogeneity of EBV epitope-specific CD8+ T cells, Michelle D. Catalina, John L. Sullivan, Robin M. Brody, and Katherine Luzuriaga
Cell surface receptors, virus entry and tropism of primate lentiviruses, Paul R. Clapham and Aine McKnight
Integrated version of reverse two-hybrid system for the postproteomic era, Hideki Endoh, Sylvie Vincent, Yves Jacob, Eleonore Real, Albertha J. M. Walhout, and Marc Vidal
Autoantibodies to Centrosomes are Diagnostic for Human Scleroderma and Can Be Induced by Experimental Mycoplasma Infection in Mice: A Dissertation, Irina Catrinel Gavanescu
Altering the Tropism of Retroviral Vectors For In Vivo Gene Therapy: Pseudotyped Virus Targeting by Ligand-Receptor Interactions: A Dissertation, Timothy J. Gollan
Redirecting retroviral tropism by insertion of short, nondisruptive peptide ligands into envelope, Timothy J. Gollan and Michael R. Green
Selective targeting and inducible destruction of human cancer cells by retroviruses with envelope proteins bearing short peptide ligands, Timothy J. Gollan and Michael R. Green
RNAi (Nematodes: Caenorhabditis elegans), Alla Grishok and Craig C. Mello
Translational control of the embryonic cell cycle, Irina Groisman, Mi-Young Jung, Madathia Sarkissian, Quiping Cao, and Joel D. Richter
Enhanced culture method for detection of replication-competent virus in peripheral blood mononuclear cells of HIV type 1-infected individuals, John P. Haran, Thomas C. Greenough, Katherine Luzuriaga, John L. Sullivan, Timothy Flanigan, and Mohan Somasundaran
TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2, Susan J. Hayes, Anil Chawla, and Silvia Corvera
MEX-3 interacting proteins link cell polarity to asymmetric gene expression in Caenorhabditis elegans, Nancy N. Huang, Darcy E. Mootz, Albertha J. M. Walhout, Marc Vidal, and Craig P. Hunter
N-methyl-D-aspartate receptor signaling results in Aurora kinase-catalyzed CPEB phosphorylation and alpha CaMKII mRNA polyadenylation at synapses, Yi-Shuian Huang, Mi-Young Jung, Madathia Sarkissian, and Joel D. Richter
The Bax subfamily of Bcl2-related proteins is essential for apoptotic signal transduction by c-Jun NH(2)-terminal kinase, Kui Lei, Anjaruwee S. Nimnual, Wei-Xing Zong, Norman J. Kennedy, Richard A. Flavell, Craig B. Thompson, Dafna Bar-Sagi, and Roger J. Davis
Pediatric HIV-1 infection: advances and remaining challenges, Katherine Luzuriaga and John L. Sullivan
Differential mRNA translation and meiotic progression require Cdc2-mediated CPEB destruction, Raul Mendez, Daron C. Barnard, and Joel D. Richter
Calculating the potential of C2 domains for membrane binding, Eric Lee Merithew and David G. Lambright
Determinants of Rab5 interaction with the N terminus of early endosome antigen 1, Eric Lee Merithew, Craig Stone, Eathiraj Sudharshan, and David G. Lambright
The intraflagellar transport protein, IFT88, is essential for vertebrate photoreceptor assembly and maintenance, Gregory J. Pazour, Sheila A. Baker, James A. Deane, Douglas G. Cole, Bethany L. Dickert, Joel L. Rosenbaum, George B. Witman, and Joseph C. Besharse
Polycystin-2 localizes to kidney cilia and the ciliary level is elevated in orpk mice with polycystic kidney disease, Gregory J. Pazour, Jovenal T. San Agustin, John A. Follit, Joel L. Rosenbaum, and George B. Witman (Current biology : CB)
Inhibition of apoptosis by ATFx: a novel role for a member of the ATF/CREB family of mammalian bZIP transcription factors, Stephan P. Persengiev, Laxminarayana R. Devireddy, and Michael R. Green
Chromatin remodeling enzymes: taming the machines. Third in review series on chromatin dynamics, Craig L. Peterson
Evidence for a cytopathogenicity determinant in HIV-1 Vpr, Mohan Somasundaran, Mark E. Sharkey, Beda Brichacek, Katherine Luzuriaga, Michael Emerman, John L. Sullivan, and Mario Stevenson
The GEX-2 and GEX-3 proteins are required for tissue morphogenesis and cell migrations in C. elegans, Martha C. Soto, Hiroshi Qadota, Katsuhisa Kasuya, Makiko Inoue, Daisuke Tsuboi, Craig C. Mello, and Kozo Kaibuchi
Arp2/3-dependent pseudocleavage [correction of psuedocleavage] furrow assembly in syncytial Drosophila embryos, Victoria A. Stevenson, Andrew M. Hudson, Lynn Cooley, and William E. Theurkauf
A selected ribozyme catalyzing diverse dipeptide synthesis, Lele Sun, Zhiyong Cui, Robert L. Gottlieb, and Biliang Zhang