Preparation and characterization of yeast cell wall beta-glucan encapsulated humic acid nanoparticles as an enhanced aflatoxin B1 binder
Program in Molecular Medicine
Biochemistry | Medicinal-Pharmaceutical Chemistry | Molecular Biology | Polymer Chemistry
This study aimed to assess the effect of encapsulating humic acid inside yeast cell walls (YCW) to detoxify AFB1 in in vitro gastrointestinal models. Glucan Mannan Lipid Particles (GMLPs) from Saccharomyces cerevisiae cell walls showed the highest AFB1 adsorption in simulated gastric fluid (SGF) after 10 min, and in simulated intestinal fluid (SIF) after 1 h. GMLPs are hollow 3-4 micron porous microspheres that provide an efficient system for the synthesis and encapsulation of AFB1-absorbing nanoparticles (NPs). Humic acid nanoparticles (HA-NPs) were synthesized within the GMLP cavity by complexation with ferric chloride. Encapsulating HA-NPs in GMLPs increased HA-NP stability in SIF. The hybrid GMLP HA-NP formulation synergistically enhanced AFB1 binding compared to individual GMLP and HA components in SGF and in SIF. Cytotoxicity on a murine macrophage cell line demonstrated that GMLP HA-NP-AFB1 complexes were stable in both SGF and SIF, detoxified AFB1 and are suitable for in vivo testing.
Aflatoxin B(1) (AFB(1)), Glucan Mannan Lipid Particle (GMLP), Humic acid (HA), In vitro gastrointestinal models, Stability and cytotoxicity, Yeast cell wall (YCW)
DOI of Published Version
Carbohydr Polym. 2019 Jan 1;203:185-192. doi: 10.1016/j.carbpol.2018.08.047. Epub 2018 Aug 12. Link to article on publisher's site
Hamza, Zeinab; El-Hashash, Maher; Aly, Soher; Hathout, Amal; Soto, Ernesto; Sabry, Bassem; and Ostroff, Gary R., "Preparation and characterization of yeast cell wall beta-glucan encapsulated humic acid nanoparticles as an enhanced aflatoxin B1 binder" (2019). Program in Molecular Medicine Publications and Presentations. 96.