Title

Preparation and characterization of yeast cell wall beta-glucan encapsulated humic acid nanoparticles as an enhanced aflatoxin B1 binder

UMMS Affiliation

Program in Molecular Medicine

Publication Date

2019-01-01

Document Type

Article

Disciplines

Biochemistry | Medicinal-Pharmaceutical Chemistry | Molecular Biology | Polymer Chemistry

Abstract

This study aimed to assess the effect of encapsulating humic acid inside yeast cell walls (YCW) to detoxify AFB1 in in vitro gastrointestinal models. Glucan Mannan Lipid Particles (GMLPs) from Saccharomyces cerevisiae cell walls showed the highest AFB1 adsorption in simulated gastric fluid (SGF) after 10 min, and in simulated intestinal fluid (SIF) after 1 h. GMLPs are hollow 3-4 micron porous microspheres that provide an efficient system for the synthesis and encapsulation of AFB1-absorbing nanoparticles (NPs). Humic acid nanoparticles (HA-NPs) were synthesized within the GMLP cavity by complexation with ferric chloride. Encapsulating HA-NPs in GMLPs increased HA-NP stability in SIF. The hybrid GMLP HA-NP formulation synergistically enhanced AFB1 binding compared to individual GMLP and HA components in SGF and in SIF. Cytotoxicity on a murine macrophage cell line demonstrated that GMLP HA-NP-AFB1 complexes were stable in both SGF and SIF, detoxified AFB1 and are suitable for in vivo testing.

Keywords

Aflatoxin B(1) (AFB(1)), Glucan Mannan Lipid Particle (GMLP), Humic acid (HA), In vitro gastrointestinal models, Stability and cytotoxicity, Yeast cell wall (YCW)

DOI of Published Version

10.1016/j.carbpol.2018.08.047

Source

Carbohydr Polym. 2019 Jan 1;203:185-192. doi: 10.1016/j.carbpol.2018.08.047. Epub 2018 Aug 12. Link to article on publisher's site

Journal/Book/Conference Title

Carbohydrate polymers

Related Resources

Link to Article in PubMed

PubMed ID

30318202

Share

COinS