Rab11 endosomes contribute to mitotic spindle organization and orientation
Program in Molecular Medicine
Biochemistry | Cell Biology | Developmental Biology
During interphase, Rab11-GTPase-containing endosomes recycle endocytic cargo. However, little is known about Rab11 endosomes in mitosis. Here, we show that Rab11 localizes to the mitotic spindle and regulates dynein-dependent endosome localization at poles. We found that mitotic recycling endosomes bind gamma-TuRC components and associate with tubulin in vitro. Rab11 depletion or dominant-negative Rab11 expression disrupts astral microtubules, delays mitosis, and redistributes spindle pole proteins. Reciprocally, constitutively active Rab11 increases astral microtubules, restores gamma-tubulin spindle pole localization, and generates robust spindles. This suggests a role for Rab11 activity in spindle pole maturation during mitosis. Rab11 depletion causes misorientation of the mitotic spindle and the plane of cell division. These findings suggest a molecular mechanism for the organization of astral microtubules and the mitotic spindle through Rab11-dependent control of spindle pole assembly and function. We propose that Rab11 and its associated endosomes cocontribute to these processes through retrograde transport to poles by dynein.
DOI of Published Version
Hehnly H, Doxsey S. Rab11 endosomes contribute to mitotic spindle organization and orientation. Dev Cell. 2014 Mar 10;28(5):497-507. doi:10.1016/j.devcel.2014.01.014. Link to article on publisher's site
Hehnly, Heidi and Doxsey, Stephen J., "Rab11 endosomes contribute to mitotic spindle organization and orientation" (2014). Program in Molecular Medicine Publications and Presentations. 7.