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Program in Molecular Medicine

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Binding Sites; Chromatin; Euchromatin; Gene Expression Regulation, Fungal; Gene Silencing; Histone-Lysine N-Methyltransferase; Methylation; Nuclear Proteins; Protein Binding; RNA Polymerase II; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins


Biochemistry | Developmental Biology | Molecular Biology | Molecular Genetics


Here we show that the Ino80 chromatin remodeling complex (Ino80C) directly prevents euchromatin from invading transcriptionally silent chromatin within intergenic regions and at the border of euchromatin and heterochromatin. Deletion of Ino80C subunits leads to increased H3K79 methylation and noncoding RNA polymerase II (Pol II) transcription centered at the Ino80C-binding sites. The effect of Ino80C is direct, as it blocks H3K79 methylation by Dot1 in vitro. Heterochromatin stimulates the binding of Ino80C in vitro and in vivo. Our data reveal that Ino80C serves as a general silencing complex that restricts transcription to gene units in euchromatin.


Dot1, H3K79 methylation, Ino80, Sir proteins, heterochromatin, silencing

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Copyright © 2015 Xue et al.; Published by Cold Spring Harbor Laboratory Press. This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at

DOI of Published Version



Genes Dev. 2015 Feb 15;29(4):350-5. doi: 10.1101/gad.256255.114. Link to article on publisher's site

Journal/Book/Conference Title

Genes and development

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Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License