Program in Molecular Medicine
Ancylostoma; Ancylostomatoidea; Ancylostomiasis; Animals; Base Sequence; Female; *Genome, Helminth; Humans; Male; Molecular Sequence Data; Multigene Family; Phylogeny; Species Specificity; *Transcriptome; Zoonoses
Bioinformatics | Computational Biology | Genetics | Genomics | Immunology and Infectious Disease | Molecular Genetics
Hookworms infect over 400 million people, stunting and impoverishing them. Sequencing hookworm genomes and finding which genes they express during infection should help in devising new drugs or vaccines against hookworms. Unlike other hookworms, Ancylostoma ceylanicum infects both humans and other mammals, providing a laboratory model for hookworm disease. We determined an A. ceylanicum genome sequence of 313 Mb, with transcriptomic data throughout infection showing expression of 30,738 genes. Approximately 900 genes were upregulated during early infection in vivo, including ASPRs, a cryptic subfamily of activation-associated secreted proteins (ASPs). Genes downregulated during early infection included ion channels and G protein-coupled receptors; this downregulation was observed in both parasitic and free-living nematodes. Later, at the onset of heavy blood feeding, C-lectin genes were upregulated along with genes for secreted clade V proteins (SCVPs), encoding a previously undescribed protein family. These findings provide new drug and vaccine targets and should help elucidate hookworm pathogenesis.
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DOI of Published Version
Nat Genet. 2015 Apr;47(4):416-22. doi: 10.1038/ng.3237. Epub 2015 Mar 2. Link to article on publisher's site
Schwarz, Erich M.; Hu, Yan; Antoshechkin, Igor; Miller, Melanie M.; Sternberg, Paul W.; and Aroian, Raffi V., "The genome and transcriptome of the zoonotic hookworm Ancylostoma ceylanicum identify infection-specific gene families" (2015). Program in Molecular Medicine Publications and Presentations. 66.
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This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.