UMMS Affiliation

Program in Molecular Medicine

Publication Date

2015-04-01

Document Type

Article

Subjects

Ancylostoma; Ancylostomatoidea; Ancylostomiasis; Animals; Base Sequence; Female; *Genome, Helminth; Humans; Male; Molecular Sequence Data; Multigene Family; Phylogeny; Species Specificity; *Transcriptome; Zoonoses

Disciplines

Bioinformatics | Computational Biology | Genetics | Genomics | Immunology and Infectious Disease | Molecular Genetics

Abstract

Hookworms infect over 400 million people, stunting and impoverishing them. Sequencing hookworm genomes and finding which genes they express during infection should help in devising new drugs or vaccines against hookworms. Unlike other hookworms, Ancylostoma ceylanicum infects both humans and other mammals, providing a laboratory model for hookworm disease. We determined an A. ceylanicum genome sequence of 313 Mb, with transcriptomic data throughout infection showing expression of 30,738 genes. Approximately 900 genes were upregulated during early infection in vivo, including ASPRs, a cryptic subfamily of activation-associated secreted proteins (ASPs). Genes downregulated during early infection included ion channels and G protein-coupled receptors; this downregulation was observed in both parasitic and free-living nematodes. Later, at the onset of heavy blood feeding, C-lectin genes were upregulated along with genes for secreted clade V proteins (SCVPs), encoding a previously undescribed protein family. These findings provide new drug and vaccine targets and should help elucidate hookworm pathogenesis.

Rights and Permissions

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/..

DOI of Published Version

10.1038/ng.3237

Source

Nat Genet. 2015 Apr;47(4):416-22. doi: 10.1038/ng.3237. Epub 2015 Mar 2. Link to article on publisher's site

Journal/Book/Conference Title

Nature genetics

Related Resources

Link to Article in PubMed

PubMed ID

25730766

Creative Commons License

Creative Commons Attribution-Noncommercial-Share Alike 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.

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