Program in Molecular Medicine
Adipocytes, Brown; Adipocytes, White; Animals; Female; Gene Expression; Gene Targeting; Homeodomain Proteins; *Homologous Recombination; Integrases; Male; Mice; Mice, Transgenic; Subcutaneous Fat
Cell Biology | Cellular and Molecular Physiology | Developmental Biology | Molecular Biology
The origins of individual fat depots are not well understood, and thus, the availability of tools useful for studying depot-specific adipose tissue development and function is limited. Cre drivers that selectively target only brown adipocyte, subcutaneous white adipocyte, or visceral white adipocyte precursors would have significant value because they could be used to selectively study individual depots without impacting the adipocyte precursors or intrinsic metabolic properties of the other depots. Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre. In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre. This suggests that Prx1-Cre-mediated recombination may be useful for making depot-restricted genetic manipulations in subcutaneous white adipocyte precursor cells, particularly when targeting genes with fat-specific functions.
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Copyright © 2015 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
DOI of Published Version
Stem Cell Reports. 2015 Apr 14;4(4):541-50. doi: 10.1016/j.stemcr.2015.02.008. Epub 2015 Mar 19. Link to article on publisher's site
Stem cell reports
Sanchez-Gurmaches, Joan; Hsiao, Wen-Yu; and Guertin, David A., "Highly selective in vivo labeling of subcutaneous white adipocyte precursors with Prx1-Cre" (2015). Program in Molecular Medicine Publications and Presentations. 52.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.