UMMS Affiliation

Program in Molecular Medicine

Publication Date

2015-04-14

Document Type

Article

Subjects

Adipocytes, Brown; Adipocytes, White; Animals; Female; Gene Expression; Gene Targeting; Homeodomain Proteins; *Homologous Recombination; Integrases; Male; Mice; Mice, Transgenic; Subcutaneous Fat

Disciplines

Cell Biology | Cellular and Molecular Physiology | Developmental Biology | Molecular Biology

Abstract

The origins of individual fat depots are not well understood, and thus, the availability of tools useful for studying depot-specific adipose tissue development and function is limited. Cre drivers that selectively target only brown adipocyte, subcutaneous white adipocyte, or visceral white adipocyte precursors would have significant value because they could be used to selectively study individual depots without impacting the adipocyte precursors or intrinsic metabolic properties of the other depots. Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre. In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre. This suggests that Prx1-Cre-mediated recombination may be useful for making depot-restricted genetic manipulations in subcutaneous white adipocyte precursor cells, particularly when targeting genes with fat-specific functions.

Rights and Permissions

Copyright © 2015 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

DOI of Published Version

10.1016/j.stemcr.2015.02.008

Source

Stem Cell Reports. 2015 Apr 14;4(4):541-50. doi: 10.1016/j.stemcr.2015.02.008. Epub 2015 Mar 19. Link to article on publisher's site

Journal/Book/Conference Title

Stem cell reports

Related Resources

Link to Article in PubMed

PubMed ID

25801508

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

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