Department of Medicine, Division of Gastroenterology; Program in Molecular Medicine
Biochemistry | Genetics and Genomics | Molecular Biology | Molecular Genetics
Circulating miRNAs can be found in extracellular vesicles (EV) and could be involved in intercellular communication. Here, we report the biodistribution of EV associated miR-155 using miR-155 KO mouse model. Administration of exosomes loaded with synthetic miR-155 mimic into miR-155 KO mice resulted in a rapid accumulation and clearance of miR-155 in the plasma with subsequent distribution in the liver, adipose tissue, lung, muscle and kidney (highest to lowest, respectively). miR-155 expression was detected in isolated hepatocytes and liver mononuclear cells of recipient KO mice suggesting its cellular uptake. In vitro, exosome-mediated restoration of miR-155 in Kupffer cells from miR-155 deficient mice augmented their LPS-induced MCP1 mRNA increase. The systemic delivery of wild type plasma to miR-155 KO mice also resulted in a rapid accumulation of miR-155 in the circulation and distribution to the liver and adipose tissue. In summary, our results demonstrate tissue biodistribution and biologic function of EV-associated miR-155.
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DOI of Published Version
Sci Rep. 2015 May 29;5:10721. doi: 10.1038/srep10721. Link to article on publisher's site
Bala, Shashi; Csak, Timea; Momen-Heravi, Fatemeh; Lippai, Dora; Kodys, Karen; Catalano, Donna; Satishchandran, Abhishek; Ambros, Victor R.; and Szabo, Gyongyi, "Biodistribution and function of extracellular miRNA-155 in mice" (2015). Program in Molecular Medicine Publications and Presentations. 41.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.