Title

The decapping scavenger enzyme DCS-1 controls microRNA levels in Caenorhabditis elegans

UMMS Affiliation

Program in Molecular Medicine

Publication Date

4-25-2013

Document Type

Article

Subjects

Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Exoribonucleases; Gene Expression; MicroRNAs; Mutation; N-Glycosyl Hydrolases; RNA Interference; RNA Stability; RNA, Helminth; RNA-Induced Silencing Complex

Disciplines

Biochemistry | Molecular Biology | Molecular Genetics

Abstract

In metazoans, microRNAs play a critical role in the posttranscriptional regulation of genes required for cell proliferation and differentiation. MicroRNAs themselves are regulated by a multitude of mechanisms influencing their transcription and posttranscriptional maturation. However, there is only sparse knowledge on pathways regulating the mature, functional form of microRNA. Here, we uncover the implication of the decapping scavenger protein DCS-1 in the control of microRNA turnover. In Caenorhabditis elegans, mutations in dcs-1 increase the levels of functional microRNAs. We demonstrate that DCS-1 interacts with the exonuclease XRN-1 to promote microRNA degradation in an independent manner from its known decapping scavenger activity, establishing two molecular functions for DCS-1. Our findings thus indicate that DCS-1 is part of a degradation complex that performs microRNA turnover in animals.

DOI of Published Version

10.1016/j.molcel.2013.02.023

Source

Mol Cell. 2013 Apr 25;50(2):281-7. doi: 10.1016/j.molcel.2013.02.023. Epub 2013 Mar 28. Link to article on publisher's site

Journal/Book/Conference Title

Molecular cell

Related Resources

Link to Article in PubMed

PubMed ID

23541767

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