Department of Emergency Medicine; Department of Medicine, Division of Cardiovascular Medicine; Program in Molecular Medicine; Department of Quantitative Health Sciences; Meyers Primary Care Institute
Biochemistry | Cardiology | Cardiovascular Diseases | Genomics | Molecular Biology | Molecular Genetics
BACKGROUND: Differences in plasma and whole blood expression microRNAs (miRNAs) in patients with an acute coronary syndrome (ACS) have been determined in both in vitro and in vivo studies. Although most circulating miRNAs are located in the cellular components of whole blood, little is known about the miRNA profiles of whole blood subcomponents, including plasma, platelets and leukocytes in patients with myocardial ischemia.
METHODS: Thirteen patients with a ST-segment-elevation (STEMI) or non-ST-segment elevation (NSTEMI) myocardial infarction were identified in the University of Massachusetts Medical Center Emergency Department (ED) or cardiac catheterization laboratory between February and June of 2012. Whole blood was obtained from arterial blood samples at the time of cardiac catheterization and cell-specific miRNA profiling was performed. Expression of 343 miRNAs was quantified from whole blood, plasma, platelets, and peripheral blood mononuclear cells using a high-throughput, quantitative Real-Time polymerase-chain reaction system (qRT-PCR).
RESULTS: MiRNAs associated with STEMI as compared to NSTEMI patients included miR-25-3p, miR-221-3p, and miR-374b-5p. MiRNA 30d-5p was associated with plasma, platelets, and leukocytes in both STEMI and NSTEMI patients; miRNAs 221-3p and 483-5p were correlated with plasma and platelets only in NSTEMI patients.
CONCLUSIONS: Cell-specific miRNA profiles differed between patients with STEMI and NSTEMI. The miRNA distribution is also unique amongst plasma, platelets, and leukocytes in patients with ischemic heart disease or ACS. Our findings suggest unique miRNA profiles among the circulating subcomponents in patients presenting with myocardial ischemia.
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Copyright: © 2013 Ward JA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
Fam Med Med Sci Res. 2013 Oct 1;2(2):108. Link to article on publisher's site
Family medicine and medical science research
Ward, Jeanine; Esa, Nada; Pidikiti, Rahul; Freedman, Jane E.; Keaney, John F.; Tanriverdi, Kahraman; Vitseva, Olga; Ambros, Victor R.; Lee, Rosalind; and McManus, David D., "Circulating Cell and Plasma microRNA Profiles Differ between Non-ST-Segment and ST-Segment-Elevation Myocardial Infarction" (2013). Program in Molecular Medicine Publications and Presentations. 22.
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