Title

Combinatorial action of NF-Y and TALE at embryonic enhancers defines distinct gene expression programs during zygotic genome activation in zebrafish

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Program in Molecular Medicine; Diabetes Center of Excellence

Publication Date

2019-12-17

Document Type

Article

Disciplines

Developmental Biology

Abstract

Animal embryogenesis is initiated by maternal factors, but zygotic genome activation (ZGA) shifts regulatory control to the embryo during blastula stages. ZGA is thought to be mediated by maternally provided transcription factors (TFs), but few such TFs have been identified in vertebrates. Here we report that NF-Y and TALE TFs bind zebrafish genomic elements associated with developmental control genes already at ZGA. In particular, co-regulation by NF-Y and TALE is associated with broadly acting genes involved in transcriptional control, while regulation by either NF-Y or TALE defines genes in specific developmental processes, such that NF-Y controls a cilia gene expression program while TALE controls expression of hox genes. We also demonstrate that NF-Y and TALE-occupied genomic elements function as enhancers during embryogenesis. We conclude that combinatorial use of NF-Y and TALE at developmental enhancers permits the establishment of distinct gene expression programs at zebrafish ZGA.

Keywords

Embryogenesis, Enhancer, Maternal, Nucleosome, Transcription, Zygotic

DOI of Published Version

10.1016/j.ydbio.2019.12.003

Source

Stanney W 3rd, Ladam F, Donaldson IJ, Parsons TJ, Maehr R, Bobola N, Sagerström CG. Combinatorial action of NF-Y and TALE at embryonic enhancers defines distinct gene expression programs during zygotic genome activation in zebrafish. Dev Biol. 2019 Dec 17;S0012-1606(19)30614-1. doi: 10.1016/j.ydbio.2019.12.003. [Epub ahead of print]. PMID: 31862379. Link to article on publisher's site

Journal/Book/Conference Title

Developmental biology

Related Resources

Link to Article in PubMed

PubMed ID

31862379

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